Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells

Recombinant baculoviruses were constructed which express simian virus 40 large T antigen (SVT-Ag) or murine p53 to high levels in infected insect cells. Characterization of the expressed proteins revealed that they display many properties of the corresponding mammalian-derived proteins. Both protein...

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Veröffentlicht in:	Journal of Virology 1988-09, Vol.62 (9), p.3109-3119
Hauptverfasser:	O'Reilly, D.R, Miller, L.K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ANTIGENE ANTIGENOS Antigens, Polyomavirus Transforming - analysis Antigens, Polyomavirus Transforming - biosynthesis Antigens, Polyomavirus Transforming - genetics Antigens, Polyomavirus Transforming - metabolism baculovirus Biological and medical sciences Cell Line Cell Nucleus - metabolism Centrifugation, Density Gradient CULTIVO DE CELULAS CULTURE DE CELLULES Electrophoresis, Polyacrylamide Gel Epitopes - analysis Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genetic Vectors Immunoassay Insect Viruses - genetics Kinetics Mice Microbiology Moths Neoplasm Proteins - analysis Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Neoplasm Proteins - metabolism Nuclear Proteins - analysis Nuclear Proteins - biosynthesis Nuclear Proteins - genetics Nuclear Proteins - metabolism Phosphoproteins - analysis Phosphoproteins - biosynthesis Phosphoproteins - genetics Phosphoproteins - metabolism Phosphorylation Plasmids PROTEINAS PROTEINE Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Simian virus 40 - genetics Simian virus 40 - immunology SPODOPTERA Spodoptera frugiperda Tumor Suppressor Protein p53 Virology VIRUS VIRUS DE INVERTEBRADOS VIRUS DES INVERTEBRES
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