DNA strand-displacement-induced fluorescence enhancement for highly sensitive and selective assay of multiple microRNA in cancer cells
We report a new strategy for evaluating multiple miRNA expressions in cancer cells based on DNA strand-displacement-induced fluorescence enhancement. This assay has the ability to discriminate the target from even single-base mismatched sequences or other miRNAs.
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Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2014-01, Vol.50 (8), p.1012-1014 |
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container_title | Chemical communications (Cambridge, England) |
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creator | Wu, Ping Tu, Yunqiu Qian, Yingdan Zhang, Hui Cai, Chenxin |
description | We report a new strategy for evaluating multiple miRNA expressions in cancer cells based on DNA strand-displacement-induced fluorescence enhancement. This assay has the ability to discriminate the target from even single-base mismatched sequences or other miRNAs. |
doi_str_mv | 10.1039/c3cc46773b |
format | Article |
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source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
subjects | Base Pair Mismatch Cell Line, Tumor DNA - metabolism Fluorescent Dyes - chemistry Graphite - chemistry Human Umbilical Vein Endothelial Cells Humans MicroRNAs - metabolism Neoplasms - genetics Neoplasms - metabolism Neoplasms - pathology Real-Time Polymerase Chain Reaction |
title | DNA strand-displacement-induced fluorescence enhancement for highly sensitive and selective assay of multiple microRNA in cancer cells |
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