Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health
Summary Leptospirosis is a zoonotic disease of global importance and one of the notifiable diseases in Sri Lanka. Recent studies on human leptospirosis have suggested that the cattle could be one of the important reservoirs for human infection in the country. However, there is a dearth of local info...
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| Veröffentlicht in: | Transboundary and emerging diseases 2014-02, Vol.61 (1), p.91-96 |
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| creator | Gamage, C. D. Koizumi, N. Perera, A. K. C. Muto, M. Nwafor-Okoli, C. Ranasinghe, S. Kularatne, S. A. M. Rajapakse, R. P. V. J. Kanda, K. Lee, R. B. Obayashi, Y. Ohnishi, M. Tamashiro, H. |
| description | Summary
Leptospirosis is a zoonotic disease of global importance and one of the notifiable diseases in Sri Lanka. Recent studies on human leptospirosis have suggested that the cattle could be one of the important reservoirs for human infection in the country. However, there is a dearth of local information on bovine leptospirosis, including its implications for human transmission. Thus, this study attempted to determine the carrier status of pathogenic Leptospira spp in cattle in Sri Lanka. A total of 164 cattle kidney samples were collected from the meat inspection hall in Colombo city during routine inspection procedures conducted by the municipal veterinary surgeons. The DNA was extracted and subjected to nested PCR for the detection of leptospiral flaB gene. Amplicons were sequenced, and phylogenic distances were calculated. Of 164 samples, 20 (12.2%) were positive for flaB‐PCR. Sequenced amplicons revealed that Leptospira species were deduced to L. borgpetersenii (10/20, 50%), L. kirschneri (7/20, 35%) and L. interrogans (3/20, 15%). The results indicate that a high proportion of the sampled cattle harbour a variety of pathogenic Leptospira spp, which can serve as important reservoirs for human disease. |
| doi_str_mv | 10.1111/tbed.12014 |
| format | Article |
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Leptospirosis is a zoonotic disease of global importance and one of the notifiable diseases in Sri Lanka. Recent studies on human leptospirosis have suggested that the cattle could be one of the important reservoirs for human infection in the country. However, there is a dearth of local information on bovine leptospirosis, including its implications for human transmission. Thus, this study attempted to determine the carrier status of pathogenic Leptospira spp in cattle in Sri Lanka. A total of 164 cattle kidney samples were collected from the meat inspection hall in Colombo city during routine inspection procedures conducted by the municipal veterinary surgeons. The DNA was extracted and subjected to nested PCR for the detection of leptospiral flaB gene. Amplicons were sequenced, and phylogenic distances were calculated. Of 164 samples, 20 (12.2%) were positive for flaB‐PCR. Sequenced amplicons revealed that Leptospira species were deduced to L. borgpetersenii (10/20, 50%), L. kirschneri (7/20, 35%) and L. interrogans (3/20, 15%). The results indicate that a high proportion of the sampled cattle harbour a variety of pathogenic Leptospira spp, which can serve as important reservoirs for human disease.</description><identifier>ISSN: 1865-1674</identifier><identifier>EISSN: 1865-1682</identifier><identifier>DOI: 10.1111/tbed.12014</identifier><identifier>PMID: 22998409</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Bacterial infections ; Carrier State - veterinary ; carrier status ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - transmission ; Disease Reservoirs - veterinary ; Disease transmission ; DNA, Bacterial - analysis ; flaB gene ; Food Inspection - standards ; Humans ; Kidney - microbiology ; Kidney - pathology ; Leptospira ; Leptospira - classification ; Leptospira - genetics ; Leptospira - isolation & purification ; leptospirosis ; Leptospirosis - blood ; Leptospirosis - epidemiology ; Leptospirosis - transmission ; Leptospirosis - veterinary ; Meat - analysis ; Polymerase Chain Reaction - veterinary ; Prevalence ; Public Health ; Sri Lanka ; Sri Lanka - epidemiology ; Zoonoses ; zoonosis</subject><ispartof>Transboundary and emerging diseases, 2014-02, Vol.61 (1), p.91-96</ispartof><rights>2012 Blackwell Verlag GmbH</rights><rights>2012 Blackwell Verlag GmbH.</rights><rights>Copyright © 2014 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4944-b3e2fbd7a2dd0df8416a953d615da4a199771b34ddbe5fb0c78003237f692c613</citedby><cites>FETCH-LOGICAL-c4944-b3e2fbd7a2dd0df8416a953d615da4a199771b34ddbe5fb0c78003237f692c613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftbed.12014$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftbed.12014$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22998409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gamage, C. D.</creatorcontrib><creatorcontrib>Koizumi, N.</creatorcontrib><creatorcontrib>Perera, A. K. C.</creatorcontrib><creatorcontrib>Muto, M.</creatorcontrib><creatorcontrib>Nwafor-Okoli, C.</creatorcontrib><creatorcontrib>Ranasinghe, S.</creatorcontrib><creatorcontrib>Kularatne, S. A. M.</creatorcontrib><creatorcontrib>Rajapakse, R. P. V. J.</creatorcontrib><creatorcontrib>Kanda, K.</creatorcontrib><creatorcontrib>Lee, R. B.</creatorcontrib><creatorcontrib>Obayashi, Y.</creatorcontrib><creatorcontrib>Ohnishi, M.</creatorcontrib><creatorcontrib>Tamashiro, H.</creatorcontrib><title>Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health</title><title>Transboundary and emerging diseases</title><addtitle>Transbound Emerg Dis</addtitle><description>Summary
Leptospirosis is a zoonotic disease of global importance and one of the notifiable diseases in Sri Lanka. Recent studies on human leptospirosis have suggested that the cattle could be one of the important reservoirs for human infection in the country. However, there is a dearth of local information on bovine leptospirosis, including its implications for human transmission. Thus, this study attempted to determine the carrier status of pathogenic Leptospira spp in cattle in Sri Lanka. A total of 164 cattle kidney samples were collected from the meat inspection hall in Colombo city during routine inspection procedures conducted by the municipal veterinary surgeons. The DNA was extracted and subjected to nested PCR for the detection of leptospiral flaB gene. Amplicons were sequenced, and phylogenic distances were calculated. Of 164 samples, 20 (12.2%) were positive for flaB‐PCR. Sequenced amplicons revealed that Leptospira species were deduced to L. borgpetersenii (10/20, 50%), L. kirschneri (7/20, 35%) and L. interrogans (3/20, 15%). The results indicate that a high proportion of the sampled cattle harbour a variety of pathogenic Leptospira spp, which can serve as important reservoirs for human disease.</description><subject>Animals</subject><subject>Bacterial infections</subject><subject>Carrier State - veterinary</subject><subject>carrier status</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - transmission</subject><subject>Disease Reservoirs - veterinary</subject><subject>Disease transmission</subject><subject>DNA, Bacterial - analysis</subject><subject>flaB gene</subject><subject>Food Inspection - standards</subject><subject>Humans</subject><subject>Kidney - microbiology</subject><subject>Kidney - pathology</subject><subject>Leptospira</subject><subject>Leptospira - classification</subject><subject>Leptospira - genetics</subject><subject>Leptospira - isolation & purification</subject><subject>leptospirosis</subject><subject>Leptospirosis - blood</subject><subject>Leptospirosis - epidemiology</subject><subject>Leptospirosis - transmission</subject><subject>Leptospirosis - veterinary</subject><subject>Meat - analysis</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Prevalence</subject><subject>Public Health</subject><subject>Sri Lanka</subject><subject>Sri Lanka - epidemiology</subject><subject>Zoonoses</subject><subject>zoonosis</subject><issn>1865-1674</issn><issn>1865-1682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9LHDEUxYO0qFVf_AAl4EspjObfJBPftqvV1qUVXBWEEjKTjEZnJ9skQ-u3b9Z1ffBBel_u5fC7B-49AOxitI9zHaTamn1MEGZrYBNXvCwwr8i7l1mwDfAhxnuEOJK8XAcbhEhZMSQ3wa-xDsHZAC-STkOEvoUTO08-zl3w0UU4mvn-Fo51Sp2FrocXwcGJ7h_0IRzBG-97n1wDp3fB6gSTh-dD3WXh1Oou3W2D963uot157lvg8uvxdHxaTH6efBuPJkXDJGNFTS1payM0MQaZtmKYa1lSw3FpNNNYSiFwTZkxtS3bGjWiQogSKlouScMx3QKflr7z4H8PNiY1c7GxXad764eoMJO8qhiR_4UigcuKLtC9V-i9H0KfD8mUYJjmL5JMfV5STX5YDLZV8-BmOjwqjNQiH7XIRz3lk-GPz5ZDPcvqCl0FkgG8BP64zj6-YaWmX46PVqbFcsfFZP--7OjwoLigolTXP05UeXZzJL5fXylE_wGXS6d5</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Gamage, C. D.</creator><creator>Koizumi, N.</creator><creator>Perera, A. K. C.</creator><creator>Muto, M.</creator><creator>Nwafor-Okoli, C.</creator><creator>Ranasinghe, S.</creator><creator>Kularatne, S. A. M.</creator><creator>Rajapakse, R. P. V. J.</creator><creator>Kanda, K.</creator><creator>Lee, R. B.</creator><creator>Obayashi, Y.</creator><creator>Ohnishi, M.</creator><creator>Tamashiro, H.</creator><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7T2</scope><scope>7U2</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>201402</creationdate><title>Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health</title><author>Gamage, C. D. ; Koizumi, N. ; Perera, A. K. C. ; Muto, M. ; Nwafor-Okoli, C. ; Ranasinghe, S. ; Kularatne, S. A. M. ; Rajapakse, R. P. V. J. ; Kanda, K. ; Lee, R. 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D.</creatorcontrib><creatorcontrib>Koizumi, N.</creatorcontrib><creatorcontrib>Perera, A. K. C.</creatorcontrib><creatorcontrib>Muto, M.</creatorcontrib><creatorcontrib>Nwafor-Okoli, C.</creatorcontrib><creatorcontrib>Ranasinghe, S.</creatorcontrib><creatorcontrib>Kularatne, S. A. M.</creatorcontrib><creatorcontrib>Rajapakse, R. P. V. J.</creatorcontrib><creatorcontrib>Kanda, K.</creatorcontrib><creatorcontrib>Lee, R. B.</creatorcontrib><creatorcontrib>Obayashi, Y.</creatorcontrib><creatorcontrib>Ohnishi, M.</creatorcontrib><creatorcontrib>Tamashiro, H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Transboundary and emerging diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gamage, C. D.</au><au>Koizumi, N.</au><au>Perera, A. K. C.</au><au>Muto, M.</au><au>Nwafor-Okoli, C.</au><au>Ranasinghe, S.</au><au>Kularatne, S. A. M.</au><au>Rajapakse, R. P. V. J.</au><au>Kanda, K.</au><au>Lee, R. B.</au><au>Obayashi, Y.</au><au>Ohnishi, M.</au><au>Tamashiro, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health</atitle><jtitle>Transboundary and emerging diseases</jtitle><addtitle>Transbound Emerg Dis</addtitle><date>2014-02</date><risdate>2014</risdate><volume>61</volume><issue>1</issue><spage>91</spage><epage>96</epage><pages>91-96</pages><issn>1865-1674</issn><eissn>1865-1682</eissn><abstract>Summary
Leptospirosis is a zoonotic disease of global importance and one of the notifiable diseases in Sri Lanka. Recent studies on human leptospirosis have suggested that the cattle could be one of the important reservoirs for human infection in the country. However, there is a dearth of local information on bovine leptospirosis, including its implications for human transmission. Thus, this study attempted to determine the carrier status of pathogenic Leptospira spp in cattle in Sri Lanka. A total of 164 cattle kidney samples were collected from the meat inspection hall in Colombo city during routine inspection procedures conducted by the municipal veterinary surgeons. The DNA was extracted and subjected to nested PCR for the detection of leptospiral flaB gene. Amplicons were sequenced, and phylogenic distances were calculated. Of 164 samples, 20 (12.2%) were positive for flaB‐PCR. Sequenced amplicons revealed that Leptospira species were deduced to L. borgpetersenii (10/20, 50%), L. kirschneri (7/20, 35%) and L. interrogans (3/20, 15%). The results indicate that a high proportion of the sampled cattle harbour a variety of pathogenic Leptospira spp, which can serve as important reservoirs for human disease.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>22998409</pmid><doi>10.1111/tbed.12014</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Bacterial infections Carrier State - veterinary carrier status Cattle Cattle Diseases - diagnosis Cattle Diseases - transmission Disease Reservoirs - veterinary Disease transmission DNA, Bacterial - analysis flaB gene Food Inspection - standards Humans Kidney - microbiology Kidney - pathology Leptospira Leptospira - classification Leptospira - genetics Leptospira - isolation & purification leptospirosis Leptospirosis - blood Leptospirosis - epidemiology Leptospirosis - transmission Leptospirosis - veterinary Meat - analysis Polymerase Chain Reaction - veterinary Prevalence Public Health Sri Lanka Sri Lanka - epidemiology Zoonoses zoonosis |
| title | Carrier Status of Leptospirosis Among Cattle in Sri Lanka: A Zoonotic Threat to Public Health |
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