Isolation and Characterization of a Transformation Deficient Mutant of Streptococcus pneumoniae that is Defective in the Production of an Activator
Transformation deficient mutants were isolated by nitrosoguanidine mutagenesis. A mutant Tra-12 was defective in the spontaneous development of competence for genetic transformation during culture growth, and showed no activator (competence inducing factor) activity inside or outside of the cells th...
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| Veröffentlicht in: | Agricultural and biological chemistry 1988-02, Vol.52 (2), p.489-494 |
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| container_end_page | 494 |
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| container_issue | 2 |
| container_start_page | 489 |
| container_title | Agricultural and biological chemistry |
| container_volume | 52 |
| creator | Fujii, Takeshi Sekimori, Yasuo Ishii, Yumi Seto, Hiroyuki |
| description | Transformation deficient mutants were isolated by nitrosoguanidine mutagenesis. A mutant Tra-12 was defective in the spontaneous development of competence for genetic transformation during culture growth, and showed no activator (competence inducing factor) activity inside or outside of the cells throughout growth. However, Tra-12 exhibited normal competence after the addition of an activator extracted from the wild type strain. This mutant needed a much larger quantity of the activator to develop a saturating level of competence and the induced competence disappeared more rapidly than in the wild type strain. |
| doi_str_mv | 10.1080/00021369.1988.10868697 |
| format | Article |
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A mutant Tra-12 was defective in the spontaneous development of competence for genetic transformation during culture growth, and showed no activator (competence inducing factor) activity inside or outside of the cells throughout growth. However, Tra-12 exhibited normal competence after the addition of an activator extracted from the wild type strain. This mutant needed a much larger quantity of the activator to develop a saturating level of competence and the induced competence disappeared more rapidly than in the wild type strain.</description><identifier>ISSN: 0002-1369</identifier><identifier>DOI: 10.1080/00021369.1988.10868697</identifier><identifier>CODEN: ABCHA6</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>Bacteriology ; Biological and medical sciences ; Fundamental and applied biological sciences. 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A mutant Tra-12 was defective in the spontaneous development of competence for genetic transformation during culture growth, and showed no activator (competence inducing factor) activity inside or outside of the cells throughout growth. However, Tra-12 exhibited normal competence after the addition of an activator extracted from the wild type strain. This mutant needed a much larger quantity of the activator to develop a saturating level of competence and the induced competence disappeared more rapidly than in the wild type strain.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. 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| ispartof | Agricultural and biological chemistry, 1988-02, Vol.52 (2), p.489-494 |
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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology Genetics Microbiology Streptococcus pneumoniae |
| title | Isolation and Characterization of a Transformation Deficient Mutant of Streptococcus pneumoniae that is Defective in the Production of an Activator |
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