Characterization of microRNA-29 family expression and investigation of their mechanistic roles in gastric cancer
Increasing evidence shows that abnormal microRNAs (miRNAs) expression is involved in tumorigenesis. They might be the novel biomarkers or therapeutic targets in disease treatment. miR-29 family was previously reported to act as tumor suppressors or oncogenes in diverse cancers. However, their accura...
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| Veröffentlicht in: | Carcinogenesis (New York) 2014-02, Vol.35 (2), p.497-506 |
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| creator | Gong, Jianan Li, Jianxiong Wang, Yi Liu, Changzheng Jia, Hongyan Jiang, Chongliang Wang, Yuxuan Luo, Min Zhao, Hongmei Dong, Lei Song, Wei Wang, Fang Wang, Weibin Zhang, Junwu Yu, Jia |
| description | Increasing evidence shows that abnormal microRNAs (miRNAs) expression is involved in tumorigenesis. They might be the novel biomarkers or therapeutic targets in disease treatment. miR-29 family was previously reported to act as tumor suppressors or oncogenes in diverse cancers. However, their accurate expression, function and mechanism in gastric cancer (GC) are not well known. Here, we found that the expression of miR-29 family members was significantly reduced in GC compared with adjacent controls. Among them, miR-29c had the most reduced percentage in GC and was associated with aggressive and progressive phenotypes of GC. We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC. Moreover, the inverse relationship between miR-29 family and their targets was verified in patients and xenograft mice. Finally, reintroduction of miR-29 family significantly inhibited tumor formation of GC cells in the xenograft mice. Take together, our finding characterized the expression properties of miR-29 family, contributed to the function and molecular mechanism of miR-29 family in GC and implied that miR-29 family might be employed as novel prognostic markers and therapeutic targets of GC. |
| doi_str_mv | 10.1093/carcin/bgt337 |
| format | Article |
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They might be the novel biomarkers or therapeutic targets in disease treatment. miR-29 family was previously reported to act as tumor suppressors or oncogenes in diverse cancers. However, their accurate expression, function and mechanism in gastric cancer (GC) are not well known. Here, we found that the expression of miR-29 family members was significantly reduced in GC compared with adjacent controls. Among them, miR-29c had the most reduced percentage in GC and was associated with aggressive and progressive phenotypes of GC. We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC. Moreover, the inverse relationship between miR-29 family and their targets was verified in patients and xenograft mice. Finally, reintroduction of miR-29 family significantly inhibited tumor formation of GC cells in the xenograft mice. Take together, our finding characterized the expression properties of miR-29 family, contributed to the function and molecular mechanism of miR-29 family in GC and implied that miR-29 family might be employed as novel prognostic markers and therapeutic targets of GC.</description><identifier>ISSN: 0143-3334</identifier><identifier>ISSN: 1460-2180</identifier><identifier>EISSN: 1460-2180</identifier><identifier>DOI: 10.1093/carcin/bgt337</identifier><identifier>PMID: 24130168</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Apoptosis ; Blotting, Northern ; Blotting, Western ; Cell Cycle ; Cell Movement ; Cell Proliferation ; Cyclin D2 - genetics ; Cyclin D2 - metabolism ; Female ; Fluorescent Antibody Technique ; Gastric Mucosa - metabolism ; Humans ; Immunoenzyme Techniques ; Matrix Metalloproteinase 2 - genetics ; Matrix Metalloproteinase 2 - metabolism ; Mice ; Mice, Nude ; MicroRNAs - genetics ; Neoplasm Invasiveness ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Stomach - pathology ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Tumor Cells, Cultured</subject><ispartof>Carcinogenesis (New York), 2014-02, Vol.35 (2), p.497-506</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-941d67a681d02a12d2e1505fb30c5967a333f6f3df20f9a4941aa06629f5ce423</citedby><cites>FETCH-LOGICAL-c332t-941d67a681d02a12d2e1505fb30c5967a333f6f3df20f9a4941aa06629f5ce423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24130168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Jianan</creatorcontrib><creatorcontrib>Li, Jianxiong</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Liu, Changzheng</creatorcontrib><creatorcontrib>Jia, Hongyan</creatorcontrib><creatorcontrib>Jiang, Chongliang</creatorcontrib><creatorcontrib>Wang, Yuxuan</creatorcontrib><creatorcontrib>Luo, Min</creatorcontrib><creatorcontrib>Zhao, Hongmei</creatorcontrib><creatorcontrib>Dong, Lei</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Wang, Weibin</creatorcontrib><creatorcontrib>Zhang, Junwu</creatorcontrib><creatorcontrib>Yu, Jia</creatorcontrib><title>Characterization of microRNA-29 family expression and investigation of their mechanistic roles in gastric cancer</title><title>Carcinogenesis (New York)</title><addtitle>Carcinogenesis</addtitle><description>Increasing evidence shows that abnormal microRNAs (miRNAs) expression is involved in tumorigenesis. They might be the novel biomarkers or therapeutic targets in disease treatment. miR-29 family was previously reported to act as tumor suppressors or oncogenes in diverse cancers. However, their accurate expression, function and mechanism in gastric cancer (GC) are not well known. Here, we found that the expression of miR-29 family members was significantly reduced in GC compared with adjacent controls. Among them, miR-29c had the most reduced percentage in GC and was associated with aggressive and progressive phenotypes of GC. We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC. Moreover, the inverse relationship between miR-29 family and their targets was verified in patients and xenograft mice. Finally, reintroduction of miR-29 family significantly inhibited tumor formation of GC cells in the xenograft mice. Take together, our finding characterized the expression properties of miR-29 family, contributed to the function and molecular mechanism of miR-29 family in GC and implied that miR-29 family might be employed as novel prognostic markers and therapeutic targets of GC.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Blotting, Northern</subject><subject>Blotting, Western</subject><subject>Cell Cycle</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Cyclin D2 - genetics</subject><subject>Cyclin D2 - metabolism</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Gastric Mucosa - metabolism</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Matrix Metalloproteinase 2 - genetics</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>MicroRNAs - genetics</subject><subject>Neoplasm Invasiveness</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Stomach - pathology</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Tumor Cells, Cultured</subject><issn>0143-3334</issn><issn>1460-2180</issn><issn>1460-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LAzEQxYMotlaPXiVHL2uTTDbtHkvxC4qC6HmZzSZtZL9MtmL9603Z2tMwM7_3mHmEXHN2x1kGU41eu2ZarHuA2QkZc6lYIvicnZIx4xISAJAjchHCJ2NcQZqdk5GQHGIzH5NuuUGPujfe_WLv2oa2ltZO-_btZZGIjFqsXbWj5qfzJoQ9gE1JXfNtQu_WR0m_Mc7T2ugNNi5uNPVtZUIE6RpD7-NAY6ONvyRnFqtgrg51Qj4e7t-XT8nq9fF5uVglGkD0SSZ5qWao5rxkArkoheEpS20BTKdZ3MS3rLJQWsFshjLyiEwpkdlUGylgQm4H3863X9t4bF67oE1VYWPabch5lABLlZIRTQY0fh2CNzbvvKvR73LO8n3I-RByPoQc-ZuD9baoTXmk_1OFP4VJeuE</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Gong, Jianan</creator><creator>Li, Jianxiong</creator><creator>Wang, Yi</creator><creator>Liu, Changzheng</creator><creator>Jia, Hongyan</creator><creator>Jiang, Chongliang</creator><creator>Wang, Yuxuan</creator><creator>Luo, Min</creator><creator>Zhao, Hongmei</creator><creator>Dong, Lei</creator><creator>Song, Wei</creator><creator>Wang, Fang</creator><creator>Wang, Weibin</creator><creator>Zhang, Junwu</creator><creator>Yu, Jia</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201402</creationdate><title>Characterization of microRNA-29 family expression and investigation of their mechanistic roles in gastric cancer</title><author>Gong, Jianan ; Li, Jianxiong ; Wang, Yi ; Liu, Changzheng ; Jia, Hongyan ; Jiang, Chongliang ; Wang, Yuxuan ; Luo, Min ; Zhao, Hongmei ; Dong, Lei ; Song, Wei ; Wang, Fang ; Wang, Weibin ; Zhang, Junwu ; Yu, Jia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-941d67a681d02a12d2e1505fb30c5967a333f6f3df20f9a4941aa06629f5ce423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Blotting, Northern</topic><topic>Blotting, Western</topic><topic>Cell Cycle</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Cyclin D2 - genetics</topic><topic>Cyclin D2 - metabolism</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Gastric Mucosa - metabolism</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Matrix Metalloproteinase 2 - genetics</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>MicroRNAs - genetics</topic><topic>Neoplasm Invasiveness</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Stomach - pathology</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Jianan</creatorcontrib><creatorcontrib>Li, Jianxiong</creatorcontrib><creatorcontrib>Wang, Yi</creatorcontrib><creatorcontrib>Liu, Changzheng</creatorcontrib><creatorcontrib>Jia, Hongyan</creatorcontrib><creatorcontrib>Jiang, Chongliang</creatorcontrib><creatorcontrib>Wang, Yuxuan</creatorcontrib><creatorcontrib>Luo, Min</creatorcontrib><creatorcontrib>Zhao, Hongmei</creatorcontrib><creatorcontrib>Dong, Lei</creatorcontrib><creatorcontrib>Song, Wei</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Wang, Weibin</creatorcontrib><creatorcontrib>Zhang, Junwu</creatorcontrib><creatorcontrib>Yu, Jia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carcinogenesis (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Jianan</au><au>Li, Jianxiong</au><au>Wang, Yi</au><au>Liu, Changzheng</au><au>Jia, Hongyan</au><au>Jiang, Chongliang</au><au>Wang, Yuxuan</au><au>Luo, Min</au><au>Zhao, Hongmei</au><au>Dong, Lei</au><au>Song, Wei</au><au>Wang, Fang</au><au>Wang, Weibin</au><au>Zhang, Junwu</au><au>Yu, Jia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of microRNA-29 family expression and investigation of their mechanistic roles in gastric cancer</atitle><jtitle>Carcinogenesis (New York)</jtitle><addtitle>Carcinogenesis</addtitle><date>2014-02</date><risdate>2014</risdate><volume>35</volume><issue>2</issue><spage>497</spage><epage>506</epage><pages>497-506</pages><issn>0143-3334</issn><issn>1460-2180</issn><eissn>1460-2180</eissn><abstract>Increasing evidence shows that abnormal microRNAs (miRNAs) expression is involved in tumorigenesis. They might be the novel biomarkers or therapeutic targets in disease treatment. miR-29 family was previously reported to act as tumor suppressors or oncogenes in diverse cancers. However, their accurate expression, function and mechanism in gastric cancer (GC) are not well known. Here, we found that the expression of miR-29 family members was significantly reduced in GC compared with adjacent controls. Among them, miR-29c had the most reduced percentage in GC and was associated with aggressive and progressive phenotypes of GC. We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC. Moreover, the inverse relationship between miR-29 family and their targets was verified in patients and xenograft mice. Finally, reintroduction of miR-29 family significantly inhibited tumor formation of GC cells in the xenograft mice. 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| subjects | Animals Apoptosis Blotting, Northern Blotting, Western Cell Cycle Cell Movement Cell Proliferation Cyclin D2 - genetics Cyclin D2 - metabolism Female Fluorescent Antibody Technique Gastric Mucosa - metabolism Humans Immunoenzyme Techniques Matrix Metalloproteinase 2 - genetics Matrix Metalloproteinase 2 - metabolism Mice Mice, Nude MicroRNAs - genetics Neoplasm Invasiveness Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Stomach - pathology Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Tumor Cells, Cultured |
| title | Characterization of microRNA-29 family expression and investigation of their mechanistic roles in gastric cancer |
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