Synthesis and Translocation of Gangliosides and Glycoproteins During Urethane Anesthesia
: In this work, we have studied (a) the contents of gangliosides, glycoproteins, and phospholipids of the vesicle and plasma membrane fractions from brains of anesthetized and control rats and chickens and (b) the labeling of gangliosides and glycoproteins in the retina ganglion cell layer and optic...
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Veröffentlicht in: | Journal of neurochemistry 1988-05, Vol.50 (5), p.1369-1374 |
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creator | Domowicz, Miriam S. Kivatinitz, Silvia C. Caputto, Beatriz L. Caputto, Ranwel |
description | : In this work, we have studied (a) the contents of gangliosides, glycoproteins, and phospholipids of the vesicle and plasma membrane fractions from brains of anesthetized and control rats and chickens and (b) the labeling of gangliosides and glycoproteins in the retina ganglion cell layer and optic tectum of urethane‐anesthetized and control chickens after intraocular injection of a labeled N‐acetylneuraminic acid precursor and the distribution of the label after subcellular fractionation. We found an increase in the content of gangliosides relative to protein in the vesicle fraction of both anesthetized rats and chickens relative to their controls. Other values were not affected by anesthesia. These results do not reflect a faster synthesis of gangliosides stimulated by urethane, because their rate of labeling was diminished in anesthetized animals. During the 4‐h period after the animals were injected intraocularly with the radioactive precursor, the highest values of ganglioside‐specific radioactivity were found in the vesicle fraction of control and anesthetized animals; at longer intervals, the specific radioactivity of the vesicle and plasma membrane fractions became rather similar. These data are in accordance with previous studies from this laboratory suggesting that the synthesis of the carbohydrate chain of gangliosides is regulated by the physiological demands made by the neurotransmitting system. |
doi_str_mv | 10.1111/j.1471-4159.1988.tb03018.x |
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We found an increase in the content of gangliosides relative to protein in the vesicle fraction of both anesthetized rats and chickens relative to their controls. Other values were not affected by anesthesia. These results do not reflect a faster synthesis of gangliosides stimulated by urethane, because their rate of labeling was diminished in anesthetized animals. During the 4‐h period after the animals were injected intraocularly with the radioactive precursor, the highest values of ganglioside‐specific radioactivity were found in the vesicle fraction of control and anesthetized animals; at longer intervals, the specific radioactivity of the vesicle and plasma membrane fractions became rather similar. These data are in accordance with previous studies from this laboratory suggesting that the synthesis of the carbohydrate chain of gangliosides is regulated by the physiological demands made by the neurotransmitting system.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1988.tb03018.x</identifier><identifier>PMID: 2834510</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Anesthesia ; Anesthetics. Neuromuscular blocking agents ; Animals ; Biochemical changes with anesthesia ; Biological and medical sciences ; Biological Transport - drug effects ; Brain - drug effects ; Brain - metabolism ; Cell Membrane - metabolism ; Chickens ; Ganglioside synthesis regulation ; Ganglioside translocation ; Gangliosides - metabolism ; Glycoproteins - metabolism ; Medical sciences ; Neuropharmacology ; Pharmacology. Drug treatments ; Phospholipids - metabolism ; Rats ; Retinal Ganglion Cells - metabolism ; Sodium-Potassium-Exchanging ATPase - metabolism ; Superior Colliculi - metabolism ; Synaptic vesicle composition ; Synaptic vesicle gangliosides ; Synaptic Vesicles - metabolism ; Tritium ; Urethane - pharmacology ; Urethane anesthesia</subject><ispartof>Journal of neurochemistry, 1988-05, Vol.50 (5), p.1369-1374</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4309-45d1248c869c90e9965c709e47136486d063f6c90ffbd973e8925ee7c87359413</citedby><cites>FETCH-LOGICAL-c4309-45d1248c869c90e9965c709e47136486d063f6c90ffbd973e8925ee7c87359413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1988.tb03018.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1988.tb03018.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7023537$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2834510$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domowicz, Miriam S.</creatorcontrib><creatorcontrib>Kivatinitz, Silvia C.</creatorcontrib><creatorcontrib>Caputto, Beatriz L.</creatorcontrib><creatorcontrib>Caputto, Ranwel</creatorcontrib><title>Synthesis and Translocation of Gangliosides and Glycoproteins During Urethane Anesthesia</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: In this work, we have studied (a) the contents of gangliosides, glycoproteins, and phospholipids of the vesicle and plasma membrane fractions from brains of anesthetized and control rats and chickens and (b) the labeling of gangliosides and glycoproteins in the retina ganglion cell layer and optic tectum of urethane‐anesthetized and control chickens after intraocular injection of a labeled N‐acetylneuraminic acid precursor and the distribution of the label after subcellular fractionation. We found an increase in the content of gangliosides relative to protein in the vesicle fraction of both anesthetized rats and chickens relative to their controls. Other values were not affected by anesthesia. These results do not reflect a faster synthesis of gangliosides stimulated by urethane, because their rate of labeling was diminished in anesthetized animals. During the 4‐h period after the animals were injected intraocularly with the radioactive precursor, the highest values of ganglioside‐specific radioactivity were found in the vesicle fraction of control and anesthetized animals; at longer intervals, the specific radioactivity of the vesicle and plasma membrane fractions became rather similar. These data are in accordance with previous studies from this laboratory suggesting that the synthesis of the carbohydrate chain of gangliosides is regulated by the physiological demands made by the neurotransmitting system.</description><subject>Anesthesia</subject><subject>Anesthetics. Neuromuscular blocking agents</subject><subject>Animals</subject><subject>Biochemical changes with anesthesia</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Chickens</subject><subject>Ganglioside synthesis regulation</subject><subject>Ganglioside translocation</subject><subject>Gangliosides - metabolism</subject><subject>Glycoproteins - metabolism</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Phospholipids - metabolism</subject><subject>Rats</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Superior Colliculi - metabolism</subject><subject>Synaptic vesicle composition</subject><subject>Synaptic vesicle gangliosides</subject><subject>Synaptic Vesicles - metabolism</subject><subject>Tritium</subject><subject>Urethane - pharmacology</subject><subject>Urethane anesthesia</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE9PwyAYh4nRzPnnI5g0xnhrhUJb8GKWqVOz6MEt8UYYpRtLB7O0cf32UtfsLhcOv-d9-fEAcI1ghPy5W0eIZCgkKGERYpRG9QJiiGi0OwLDQ3QMhhDGcYghiU_BmXNrCFFKUjQAg5hikiA4BF-fralXymkXCJMHs0oYV1opam1NYItgIsyy1NbpXO2JSdlKu61srbRxwWNTabMM5pWqV8KoYGSU-1snLsBJIUqnLvv7HMyfn2bjl3D6MXkdj6ahJBiykCQ5igmVNGWSQcVYmsgMMuU_gVNC0xymuEh9VBSLnGVYURYnSmWSZjhhBOFzcLvf6zt9N_51vtFOqrL0dWzjOCKeIjjx4P0elJV1rlIF31Z6I6qWI8g7rXzNO3e8c8c7rbzXynd--Kp_pVlsVH4Y7T36_KbPhZOiLLxGqd0By2DsG2Qee9hjP7pU7T8K8Lf3sRfC8C_xd5RO</recordid><startdate>198805</startdate><enddate>198805</enddate><creator>Domowicz, Miriam S.</creator><creator>Kivatinitz, Silvia C.</creator><creator>Caputto, Beatriz L.</creator><creator>Caputto, Ranwel</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>198805</creationdate><title>Synthesis and Translocation of Gangliosides and Glycoproteins During Urethane Anesthesia</title><author>Domowicz, Miriam S. ; Kivatinitz, Silvia C. ; Caputto, Beatriz L. ; Caputto, Ranwel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4309-45d1248c869c90e9965c709e47136486d063f6c90ffbd973e8925ee7c87359413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Anesthesia</topic><topic>Anesthetics. Neuromuscular blocking agents</topic><topic>Animals</topic><topic>Biochemical changes with anesthesia</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Chickens</topic><topic>Ganglioside synthesis regulation</topic><topic>Ganglioside translocation</topic><topic>Gangliosides - metabolism</topic><topic>Glycoproteins - metabolism</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Phospholipids - metabolism</topic><topic>Rats</topic><topic>Retinal Ganglion Cells - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Superior Colliculi - metabolism</topic><topic>Synaptic vesicle composition</topic><topic>Synaptic vesicle gangliosides</topic><topic>Synaptic Vesicles - metabolism</topic><topic>Tritium</topic><topic>Urethane - pharmacology</topic><topic>Urethane anesthesia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Domowicz, Miriam S.</creatorcontrib><creatorcontrib>Kivatinitz, Silvia C.</creatorcontrib><creatorcontrib>Caputto, Beatriz L.</creatorcontrib><creatorcontrib>Caputto, Ranwel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Domowicz, Miriam S.</au><au>Kivatinitz, Silvia C.</au><au>Caputto, Beatriz L.</au><au>Caputto, Ranwel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and Translocation of Gangliosides and Glycoproteins During Urethane Anesthesia</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1988-05</date><risdate>1988</risdate><volume>50</volume><issue>5</issue><spage>1369</spage><epage>1374</epage><pages>1369-1374</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: In this work, we have studied (a) the contents of gangliosides, glycoproteins, and phospholipids of the vesicle and plasma membrane fractions from brains of anesthetized and control rats and chickens and (b) the labeling of gangliosides and glycoproteins in the retina ganglion cell layer and optic tectum of urethane‐anesthetized and control chickens after intraocular injection of a labeled N‐acetylneuraminic acid precursor and the distribution of the label after subcellular fractionation. We found an increase in the content of gangliosides relative to protein in the vesicle fraction of both anesthetized rats and chickens relative to their controls. Other values were not affected by anesthesia. These results do not reflect a faster synthesis of gangliosides stimulated by urethane, because their rate of labeling was diminished in anesthetized animals. During the 4‐h period after the animals were injected intraocularly with the radioactive precursor, the highest values of ganglioside‐specific radioactivity were found in the vesicle fraction of control and anesthetized animals; at longer intervals, the specific radioactivity of the vesicle and plasma membrane fractions became rather similar. These data are in accordance with previous studies from this laboratory suggesting that the synthesis of the carbohydrate chain of gangliosides is regulated by the physiological demands made by the neurotransmitting system.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>2834510</pmid><doi>10.1111/j.1471-4159.1988.tb03018.x</doi><tpages>6</tpages></addata></record> |
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subjects | Anesthesia Anesthetics. Neuromuscular blocking agents Animals Biochemical changes with anesthesia Biological and medical sciences Biological Transport - drug effects Brain - drug effects Brain - metabolism Cell Membrane - metabolism Chickens Ganglioside synthesis regulation Ganglioside translocation Gangliosides - metabolism Glycoproteins - metabolism Medical sciences Neuropharmacology Pharmacology. Drug treatments Phospholipids - metabolism Rats Retinal Ganglion Cells - metabolism Sodium-Potassium-Exchanging ATPase - metabolism Superior Colliculi - metabolism Synaptic vesicle composition Synaptic vesicle gangliosides Synaptic Vesicles - metabolism Tritium Urethane - pharmacology Urethane anesthesia |
title | Synthesis and Translocation of Gangliosides and Glycoproteins During Urethane Anesthesia |
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