Characterization of Simulium (Edwardsellum) damnosum s. l. populations from six river systems in Kenya by cellulose acetate electrophoresis
Isoenzyme characterization of the Simulium damnosum Theobald sibling species complex from two widely separated geographical areas in Kenya is presented based on 10 enzymatic loci. Four river systems in Western and Nyanza Provinces, namely, the Yala, Lusumu, Isiukhu and the Nzoia harbouring S. damnos...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1986, Vol.80 (6), p.914-922 |
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| creator | Mebrahtu, Yemane Beach, Raymond F. Khamala, C.P.M. Hendricks, L.D. |
| description | Isoenzyme characterization of the
Simulium damnosum Theobald sibling species complex from two widely separated geographical areas in Kenya is presented based on 10 enzymatic loci. Four river systems in Western and Nyanza Provinces, namely, the Yala, Lusumu, Isiukhu and the Nzoia harbouring
S. damnosum s.l. were compared among themselves and with
S. damnosum s.l. collected from the Thiba and the Nyamindi river systems in the Mt. Kenya area. The two populations were easily separable using PGM, HK and, more than 73% of the time, with PGI. Using the first two enzymatic loci, PGM and HK, all the western Kenya
S. damnosum s.l. belong to the same population while those from Mt. Kenya areas belong to a different population. In both geographical zones there was less than 20% qualitative and quantitative polymorphism within infraspecific forms in any given breeding area of
S. damnosum s.l. Three enzymes, ME, XDH, and G-6-PDH had isomorphic mobilities for both the Mt. Kenya and western Kenya populations. Four other enzyme/substrate systems tested had no satisfactory resolution as a diagnostic value. |
| doi_str_mv | 10.1016/0035-9203(86)90258-0 |
| format | Article |
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Simulium damnosum Theobald sibling species complex from two widely separated geographical areas in Kenya is presented based on 10 enzymatic loci. Four river systems in Western and Nyanza Provinces, namely, the Yala, Lusumu, Isiukhu and the Nzoia harbouring
S. damnosum s.l. were compared among themselves and with
S. damnosum s.l. collected from the Thiba and the Nyamindi river systems in the Mt. Kenya area. The two populations were easily separable using PGM, HK and, more than 73% of the time, with PGI. Using the first two enzymatic loci, PGM and HK, all the western Kenya
S. damnosum s.l. belong to the same population while those from Mt. Kenya areas belong to a different population. In both geographical zones there was less than 20% qualitative and quantitative polymorphism within infraspecific forms in any given breeding area of
S. damnosum s.l. Three enzymes, ME, XDH, and G-6-PDH had isomorphic mobilities for both the Mt. Kenya and western Kenya populations. Four other enzyme/substrate systems tested had no satisfactory resolution as a diagnostic value.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/0035-9203(86)90258-0</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>chemotaxonomy ; disease diagnosis ; electrophoresis ; enzyme polymorphism ; identification ; isozymes ; Simuliidae ; Simulium damnosum</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 1986, Vol.80 (6), p.914-922</ispartof><rights>1986</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2440-52937d50367a14eaa6b1412532356ff4b76d17aa15713680b2e196476494cf513</citedby><cites>FETCH-LOGICAL-c2440-52937d50367a14eaa6b1412532356ff4b76d17aa15713680b2e196476494cf513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids></links><search><creatorcontrib>Mebrahtu, Yemane</creatorcontrib><creatorcontrib>Beach, Raymond F.</creatorcontrib><creatorcontrib>Khamala, C.P.M.</creatorcontrib><creatorcontrib>Hendricks, L.D.</creatorcontrib><title>Characterization of Simulium (Edwardsellum) damnosum s. l. populations from six river systems in Kenya by cellulose acetate electrophoresis</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>Isoenzyme characterization of the
Simulium damnosum Theobald sibling species complex from two widely separated geographical areas in Kenya is presented based on 10 enzymatic loci. Four river systems in Western and Nyanza Provinces, namely, the Yala, Lusumu, Isiukhu and the Nzoia harbouring
S. damnosum s.l. were compared among themselves and with
S. damnosum s.l. collected from the Thiba and the Nyamindi river systems in the Mt. Kenya area. The two populations were easily separable using PGM, HK and, more than 73% of the time, with PGI. Using the first two enzymatic loci, PGM and HK, all the western Kenya
S. damnosum s.l. belong to the same population while those from Mt. Kenya areas belong to a different population. In both geographical zones there was less than 20% qualitative and quantitative polymorphism within infraspecific forms in any given breeding area of
S. damnosum s.l. Three enzymes, ME, XDH, and G-6-PDH had isomorphic mobilities for both the Mt. Kenya and western Kenya populations. Four other enzyme/substrate systems tested had no satisfactory resolution as a diagnostic value.</description><subject>chemotaxonomy</subject><subject>disease diagnosis</subject><subject>electrophoresis</subject><subject>enzyme polymorphism</subject><subject>identification</subject><subject>isozymes</subject><subject>Simuliidae</subject><subject>Simulium damnosum</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNp9kM1u1DAURi0EEsPAGyDhFWoXGez4L9kgoWmhiAISQwXqxvIkN9SQxME3KR1egZfGaVCXrCxdne_zvYeQp5xtOOP6BWNCZWXOxFGhj0uWqyJj98iKF6bIhGLiPlndIQ_JI8TvLEFclSvyZ3vloqtGiP63G33oaWjozndT66eOHp3Wv1ysEdp26o5p7bo-YJrjhrYbOoRham9DSJsY0tjf0OivIVI84AgdUt_Td9AfHN0faDW3tAGBugpGNwKFFqoxhuEqRECPj8mDxrUIT_69a3Lx-vTz9iw7__jm7fbVeVblUrJM5aUwdTpLG8clOKf3XPJciVwo3TRyb3TNjXNcGS50wfY58FJLo2Upq0ZxsSbPl94hhp8T4Gg7j_N2rocwoeWyzAuTPlgTuYBVDIgRGjtE37l4sJzZ2bydtdpZqy20vTVv51i2xHyScHOXcfGH1UYYZc--Xtr3n052HwS7tF8S_2zhGxes-xY92otdzrhgXKZF2Nz4ciEgabn2EC1WHvoKah-TQlsH__-V_gITUqP7</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Mebrahtu, Yemane</creator><creator>Beach, Raymond F.</creator><creator>Khamala, C.P.M.</creator><creator>Hendricks, L.D.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope></search><sort><creationdate>1986</creationdate><title>Characterization of Simulium (Edwardsellum) damnosum s. l. populations from six river systems in Kenya by cellulose acetate electrophoresis</title><author>Mebrahtu, Yemane ; Beach, Raymond F. ; Khamala, C.P.M. ; Hendricks, L.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2440-52937d50367a14eaa6b1412532356ff4b76d17aa15713680b2e196476494cf513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>chemotaxonomy</topic><topic>disease diagnosis</topic><topic>electrophoresis</topic><topic>enzyme polymorphism</topic><topic>identification</topic><topic>isozymes</topic><topic>Simuliidae</topic><topic>Simulium damnosum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mebrahtu, Yemane</creatorcontrib><creatorcontrib>Beach, Raymond F.</creatorcontrib><creatorcontrib>Khamala, C.P.M.</creatorcontrib><creatorcontrib>Hendricks, L.D.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mebrahtu, Yemane</au><au>Beach, Raymond F.</au><au>Khamala, C.P.M.</au><au>Hendricks, L.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Simulium (Edwardsellum) damnosum s. l. populations from six river systems in Kenya by cellulose acetate electrophoresis</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>1986</date><risdate>1986</risdate><volume>80</volume><issue>6</issue><spage>914</spage><epage>922</epage><pages>914-922</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>Isoenzyme characterization of the
Simulium damnosum Theobald sibling species complex from two widely separated geographical areas in Kenya is presented based on 10 enzymatic loci. Four river systems in Western and Nyanza Provinces, namely, the Yala, Lusumu, Isiukhu and the Nzoia harbouring
S. damnosum s.l. were compared among themselves and with
S. damnosum s.l. collected from the Thiba and the Nyamindi river systems in the Mt. Kenya area. The two populations were easily separable using PGM, HK and, more than 73% of the time, with PGI. Using the first two enzymatic loci, PGM and HK, all the western Kenya
S. damnosum s.l. belong to the same population while those from Mt. Kenya areas belong to a different population. In both geographical zones there was less than 20% qualitative and quantitative polymorphism within infraspecific forms in any given breeding area of
S. damnosum s.l. Three enzymes, ME, XDH, and G-6-PDH had isomorphic mobilities for both the Mt. Kenya and western Kenya populations. Four other enzyme/substrate systems tested had no satisfactory resolution as a diagnostic value.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/0035-9203(86)90258-0</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1986, Vol.80 (6), p.914-922 |
| issn | 0035-9203 1878-3503 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14928750 |
| source | Alma/SFX Local Collection |
| subjects | chemotaxonomy disease diagnosis electrophoresis enzyme polymorphism identification isozymes Simuliidae Simulium damnosum |
| title | Characterization of Simulium (Edwardsellum) damnosum s. l. populations from six river systems in Kenya by cellulose acetate electrophoresis |
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