Construction of carotenoid biosynthetic pathways using squalene synthase
•Squalene synthase and carotenoid desaturase CrtN produced carotenoid pigments.•Various experiments suggested CrtN desaturates squalene into dehydrosqualene.•Possible existence of “squalene/lycopersene-to-carotenoid route” was suggested.•A new method of color screening for squalene synthase activity...
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| Veröffentlicht in: | FEBS letters 2014-01, Vol.588 (3), p.436-442 |
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| creator | Furubayashi, Maiko Li, Ling Katabami, Akinori Saito, Kyoichi Umeno, Daisuke |
| description | •Squalene synthase and carotenoid desaturase CrtN produced carotenoid pigments.•Various experiments suggested CrtN desaturates squalene into dehydrosqualene.•Possible existence of “squalene/lycopersene-to-carotenoid route” was suggested.•A new method of color screening for squalene synthase activity was proposed.
The first committed steps of steroid/hopanoid pathways involve squalene synthase (SQS). Here, we report the Escherichia coli production of diaponeurosporene and diapolycopene, yellow C30 carotenoid pigments, by expressing human SQS and Staphylococcus aureus dehydrosqualene (C30 carotenoid) desaturase (CrtN). We suggest that the carotenoid pigments are synthesized mainly via the desaturation of squalene rather than the direct synthesis of dehydrosqualene through the non-reductive condensation of prenyl diphosphate precursors, indicating the possible existence of a “squalene route” and a “lycopersene route” for C30 and C40 carotenoids, respectively. Additionally, this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs. |
| doi_str_mv | 10.1016/j.febslet.2013.12.003 |
| format | Article |
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The first committed steps of steroid/hopanoid pathways involve squalene synthase (SQS). Here, we report the Escherichia coli production of diaponeurosporene and diapolycopene, yellow C30 carotenoid pigments, by expressing human SQS and Staphylococcus aureus dehydrosqualene (C30 carotenoid) desaturase (CrtN). We suggest that the carotenoid pigments are synthesized mainly via the desaturation of squalene rather than the direct synthesis of dehydrosqualene through the non-reductive condensation of prenyl diphosphate precursors, indicating the possible existence of a “squalene route” and a “lycopersene route” for C30 and C40 carotenoids, respectively. Additionally, this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2013.12.003</identifier><identifier>PMID: 24333579</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Bacterial Proteins ; Biosynthetic Pathways ; Carotenoid ; Carotenoids - biosynthesis ; Carotenoids - chemistry ; Carotenoids - genetics ; Chimeric pathway ; CrtB ; CrtI ; CrtM ; CrtN ; dehydrosqualene desaturase ; dehydrosqualene synthase ; Desaturase ; Escherichia coli - enzymology ; farnesyl diphosphate ; Farnesyl-Diphosphate Farnesyltransferase - chemistry ; Farnesyl-Diphosphate Farnesyltransferase - genetics ; Farnesyl-Diphosphate Farnesyltransferase - metabolism ; FPP ; geranylgeranyl diphosphate ; GGPP ; hSQS ; human squalene synthase ; Humans ; Lycopersene ; Oxidoreductases ; phytoene desaturase ; phytoene synthase ; presqualene diphosphate ; PSPP ; SQS ; Squalene ; squalene synthase ; Staphylococcus aureus - enzymology</subject><ispartof>FEBS letters, 2014-01, Vol.588 (3), p.436-442</ispartof><rights>2013 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4963-66ed7a81f611363e49ff18fed15e17556e5f3b7ea72bb12748bf7480e973cec43</citedby><cites>FETCH-LOGICAL-c4963-66ed7a81f611363e49ff18fed15e17556e5f3b7ea72bb12748bf7480e973cec43</cites><orcidid>0000-0001-5215-523X ; 0000-0003-2192-4678</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2013.12.003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2013.12.003$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,1432,3548,27923,27924,45573,45574,45994,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24333579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furubayashi, Maiko</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>Katabami, Akinori</creatorcontrib><creatorcontrib>Saito, Kyoichi</creatorcontrib><creatorcontrib>Umeno, Daisuke</creatorcontrib><title>Construction of carotenoid biosynthetic pathways using squalene synthase</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Squalene synthase and carotenoid desaturase CrtN produced carotenoid pigments.•Various experiments suggested CrtN desaturates squalene into dehydrosqualene.•Possible existence of “squalene/lycopersene-to-carotenoid route” was suggested.•A new method of color screening for squalene synthase activity was proposed.
The first committed steps of steroid/hopanoid pathways involve squalene synthase (SQS). Here, we report the Escherichia coli production of diaponeurosporene and diapolycopene, yellow C30 carotenoid pigments, by expressing human SQS and Staphylococcus aureus dehydrosqualene (C30 carotenoid) desaturase (CrtN). We suggest that the carotenoid pigments are synthesized mainly via the desaturation of squalene rather than the direct synthesis of dehydrosqualene through the non-reductive condensation of prenyl diphosphate precursors, indicating the possible existence of a “squalene route” and a “lycopersene route” for C30 and C40 carotenoids, respectively. Additionally, this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs.</description><subject>Bacterial Proteins</subject><subject>Biosynthetic Pathways</subject><subject>Carotenoid</subject><subject>Carotenoids - biosynthesis</subject><subject>Carotenoids - chemistry</subject><subject>Carotenoids - genetics</subject><subject>Chimeric pathway</subject><subject>CrtB</subject><subject>CrtI</subject><subject>CrtM</subject><subject>CrtN</subject><subject>dehydrosqualene desaturase</subject><subject>dehydrosqualene synthase</subject><subject>Desaturase</subject><subject>Escherichia coli - enzymology</subject><subject>farnesyl diphosphate</subject><subject>Farnesyl-Diphosphate Farnesyltransferase - chemistry</subject><subject>Farnesyl-Diphosphate Farnesyltransferase - genetics</subject><subject>Farnesyl-Diphosphate Farnesyltransferase - metabolism</subject><subject>FPP</subject><subject>geranylgeranyl diphosphate</subject><subject>GGPP</subject><subject>hSQS</subject><subject>human squalene synthase</subject><subject>Humans</subject><subject>Lycopersene</subject><subject>Oxidoreductases</subject><subject>phytoene desaturase</subject><subject>phytoene synthase</subject><subject>presqualene diphosphate</subject><subject>PSPP</subject><subject>SQS</subject><subject>Squalene</subject><subject>squalene synthase</subject><subject>Staphylococcus aureus - enzymology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUGP1CAUx4nRuOPqR9D06KWVBxToyehk1zHZxIN6JpQ-XCadMgvUzXx7W2f0qhdeCL_3f-T3CHkNtAEK8t2-8djnEUvDKPAGWEMpf0I2oBWvuZD6KdlQCqJuVcevyIuc93S5a-iekysmOOfLw4bstnHKJc2uhDhV0VfOplhwimGo-hDzaSr3WIKrjrbcP9pTruYcph9VfpjtiBNWvwmb8SV55u2Y8dWlXpPvtzfftrv67sunz9sPd7UTneS1lDgoq8FLAC45is570B4HaBFU20psPe8VWsX6HpgSuvfLQbFT3KET_Jq8PeceU3yYMRdzCNnhONoJ45wNiI4pkJqyBW3PqEsx54TeHFM42HQyQM0q0ezNRaJZJRpgZpG49L25jJj7Aw5_u_5YW4DdGXgMI57-L9Xc3nxkX9eNrAnAKdVarLPen6NwcfYzYDLZBZwcDiGhK2aI4R-__QXREJv5</recordid><startdate>20140131</startdate><enddate>20140131</enddate><creator>Furubayashi, Maiko</creator><creator>Li, Ling</creator><creator>Katabami, Akinori</creator><creator>Saito, Kyoichi</creator><creator>Umeno, Daisuke</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5215-523X</orcidid><orcidid>https://orcid.org/0000-0003-2192-4678</orcidid></search><sort><creationdate>20140131</creationdate><title>Construction of carotenoid biosynthetic pathways using squalene synthase</title><author>Furubayashi, Maiko ; Li, Ling ; Katabami, Akinori ; Saito, Kyoichi ; Umeno, Daisuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4963-66ed7a81f611363e49ff18fed15e17556e5f3b7ea72bb12748bf7480e973cec43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacterial Proteins</topic><topic>Biosynthetic Pathways</topic><topic>Carotenoid</topic><topic>Carotenoids - biosynthesis</topic><topic>Carotenoids - chemistry</topic><topic>Carotenoids - genetics</topic><topic>Chimeric pathway</topic><topic>CrtB</topic><topic>CrtI</topic><topic>CrtM</topic><topic>CrtN</topic><topic>dehydrosqualene desaturase</topic><topic>dehydrosqualene synthase</topic><topic>Desaturase</topic><topic>Escherichia coli - enzymology</topic><topic>farnesyl diphosphate</topic><topic>Farnesyl-Diphosphate Farnesyltransferase - chemistry</topic><topic>Farnesyl-Diphosphate Farnesyltransferase - genetics</topic><topic>Farnesyl-Diphosphate Farnesyltransferase - metabolism</topic><topic>FPP</topic><topic>geranylgeranyl diphosphate</topic><topic>GGPP</topic><topic>hSQS</topic><topic>human squalene synthase</topic><topic>Humans</topic><topic>Lycopersene</topic><topic>Oxidoreductases</topic><topic>phytoene desaturase</topic><topic>phytoene synthase</topic><topic>presqualene diphosphate</topic><topic>PSPP</topic><topic>SQS</topic><topic>Squalene</topic><topic>squalene synthase</topic><topic>Staphylococcus aureus - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furubayashi, Maiko</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>Katabami, Akinori</creatorcontrib><creatorcontrib>Saito, Kyoichi</creatorcontrib><creatorcontrib>Umeno, Daisuke</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furubayashi, Maiko</au><au>Li, Ling</au><au>Katabami, Akinori</au><au>Saito, Kyoichi</au><au>Umeno, Daisuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of carotenoid biosynthetic pathways using squalene synthase</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-01-31</date><risdate>2014</risdate><volume>588</volume><issue>3</issue><spage>436</spage><epage>442</epage><pages>436-442</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Squalene synthase and carotenoid desaturase CrtN produced carotenoid pigments.•Various experiments suggested CrtN desaturates squalene into dehydrosqualene.•Possible existence of “squalene/lycopersene-to-carotenoid route” was suggested.•A new method of color screening for squalene synthase activity was proposed.
The first committed steps of steroid/hopanoid pathways involve squalene synthase (SQS). Here, we report the Escherichia coli production of diaponeurosporene and diapolycopene, yellow C30 carotenoid pigments, by expressing human SQS and Staphylococcus aureus dehydrosqualene (C30 carotenoid) desaturase (CrtN). We suggest that the carotenoid pigments are synthesized mainly via the desaturation of squalene rather than the direct synthesis of dehydrosqualene through the non-reductive condensation of prenyl diphosphate precursors, indicating the possible existence of a “squalene route” and a “lycopersene route” for C30 and C40 carotenoids, respectively. Additionally, this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>24333579</pmid><doi>10.1016/j.febslet.2013.12.003</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5215-523X</orcidid><orcidid>https://orcid.org/0000-0003-2192-4678</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2014-01, Vol.588 (3), p.436-442 |
| issn | 0014-5793 1873-3468 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bacterial Proteins Biosynthetic Pathways Carotenoid Carotenoids - biosynthesis Carotenoids - chemistry Carotenoids - genetics Chimeric pathway CrtB CrtI CrtM CrtN dehydrosqualene desaturase dehydrosqualene synthase Desaturase Escherichia coli - enzymology farnesyl diphosphate Farnesyl-Diphosphate Farnesyltransferase - chemistry Farnesyl-Diphosphate Farnesyltransferase - genetics Farnesyl-Diphosphate Farnesyltransferase - metabolism FPP geranylgeranyl diphosphate GGPP hSQS human squalene synthase Humans Lycopersene Oxidoreductases phytoene desaturase phytoene synthase presqualene diphosphate PSPP SQS Squalene squalene synthase Staphylococcus aureus - enzymology |
| title | Construction of carotenoid biosynthetic pathways using squalene synthase |
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