Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section
Aim To investigate the developmental competence of human embryos that originated from in vitro matured oocytes retrieved during cesarean section. Methods Immature oocytes were collected from 95 pregnant women, who underwent cesarean section at Buddhachinaraj Hospital Medical School and consented to...
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| Veröffentlicht in: | The journal of obstetrics and gynaecology research 2014-02, Vol.40 (2), p.459-464 |
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| container_title | The journal of obstetrics and gynaecology research |
| container_volume | 40 |
| creator | Pongsuthirak, Pallop Vutyavanich, Teraporn |
| description | Aim
To investigate the developmental competence of human embryos that originated from in vitro matured oocytes retrieved during cesarean section.
Methods
Immature oocytes were collected from 95 pregnant women, who underwent cesarean section at Buddhachinaraj Hospital Medical School and consented to participate in the study. Retrieved oocytes were cultured in blastocyst medium supplemented with 75 IU/L of human menopausal gonadotropin. Oocyte maturation was assessed at 30 and 48 h after culture. In vitro matured oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 h. The fertilization, cleavage and blastocyst formation rates were observed.
Results
Maturation rate of oocytes after 30 h of culture was 67.9% compared with 13.1% at 48 h (P |
| doi_str_mv | 10.1111/jog.12186 |
| format | Article |
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To investigate the developmental competence of human embryos that originated from in vitro matured oocytes retrieved during cesarean section.
Methods
Immature oocytes were collected from 95 pregnant women, who underwent cesarean section at Buddhachinaraj Hospital Medical School and consented to participate in the study. Retrieved oocytes were cultured in blastocyst medium supplemented with 75 IU/L of human menopausal gonadotropin. Oocyte maturation was assessed at 30 and 48 h after culture. In vitro matured oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 h. The fertilization, cleavage and blastocyst formation rates were observed.
Results
Maturation rate of oocytes after 30 h of culture was 67.9% compared with 13.1% at 48 h (P < 0.0001). Insemination of oocytes in both groups resulted in similar fertilization, cleavage and blastocyst formation rates.
Conclusion
A large proportion of oocytes retrieved at the time of cesarean section exhibited the capacity to undergo maturation in vitro. They can be fertilized and developed into good‐quality blastocyst stage.</description><identifier>ISSN: 1341-8076</identifier><identifier>EISSN: 1447-0756</identifier><identifier>DOI: 10.1111/jog.12186</identifier><identifier>PMID: 24118307</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>Adolescent ; Adult ; Blastocyst - physiology ; Cesarean Section ; cytoplasmic maturation ; Embryo Culture Techniques ; Embryonic Development - physiology ; Embryos ; Female ; Fertilization ; Fertilization - physiology ; Gonadotropins ; Humans ; in vitro maturation ; Maturation ; nuclear maturation ; Oocyte Retrieval ; Oocytes ; Pituitary (anterior) ; Pregnancy ; Sperm Injections, Intracytoplasmic ; Young Adult</subject><ispartof>The journal of obstetrics and gynaecology research, 2014-02, Vol.40 (2), p.459-464</ispartof><rights>2013 The Authors. Journal of Obstetrics and Gynaecology Research © 2013 Japan Society of Obstetrics and Gynecology</rights><rights>2013 The Authors. Journal of Obstetrics and Gynaecology Research © 2013 Japan Society of Obstetrics and Gynecology.</rights><rights>Copyright © 2014 Japan Society of Obstetrics and Gynecology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4156-4c5bbd631880756fd17e3aefd87786db99c34c449559fcf0c7f2fc31802d0ba3</citedby><cites>FETCH-LOGICAL-c4156-4c5bbd631880756fd17e3aefd87786db99c34c449559fcf0c7f2fc31802d0ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjog.12186$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjog.12186$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24118307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pongsuthirak, Pallop</creatorcontrib><creatorcontrib>Vutyavanich, Teraporn</creatorcontrib><title>Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section</title><title>The journal of obstetrics and gynaecology research</title><addtitle>J Obstet Gynaecol Res</addtitle><description>Aim
To investigate the developmental competence of human embryos that originated from in vitro matured oocytes retrieved during cesarean section.
Methods
Immature oocytes were collected from 95 pregnant women, who underwent cesarean section at Buddhachinaraj Hospital Medical School and consented to participate in the study. Retrieved oocytes were cultured in blastocyst medium supplemented with 75 IU/L of human menopausal gonadotropin. Oocyte maturation was assessed at 30 and 48 h after culture. In vitro matured oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 h. The fertilization, cleavage and blastocyst formation rates were observed.
Results
Maturation rate of oocytes after 30 h of culture was 67.9% compared with 13.1% at 48 h (P < 0.0001). Insemination of oocytes in both groups resulted in similar fertilization, cleavage and blastocyst formation rates.
Conclusion
A large proportion of oocytes retrieved at the time of cesarean section exhibited the capacity to undergo maturation in vitro. They can be fertilized and developed into good‐quality blastocyst stage.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Blastocyst - physiology</subject><subject>Cesarean Section</subject><subject>cytoplasmic maturation</subject><subject>Embryo Culture Techniques</subject><subject>Embryonic Development - physiology</subject><subject>Embryos</subject><subject>Female</subject><subject>Fertilization</subject><subject>Fertilization - physiology</subject><subject>Gonadotropins</subject><subject>Humans</subject><subject>in vitro maturation</subject><subject>Maturation</subject><subject>nuclear maturation</subject><subject>Oocyte Retrieval</subject><subject>Oocytes</subject><subject>Pituitary (anterior)</subject><subject>Pregnancy</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Young Adult</subject><issn>1341-8076</issn><issn>1447-0756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAURSMEoqWw4AeQJTZlkdYvduJkCW0ZaEsroUosLcd5HjzE8WAnA7Pj03Fm2i6Q8Ma2dM7Vs2-WvQZ6AmmdrvzyBAqoqyfZIXAucirK6mk6Mw55TUV1kL2IcUUpiAbq59lBwQFqRsVh9uccN9j7tcNhVD3R3q1xxEEj8YZ8n5waCLo2bH0kHQa7wY6Y4B2xA9nYMXji1DgFNVo_zIZ1u3uyvd6OGEnAMVictW4KdlgSjVEFTLER9Wy9zJ4Z1Ud8db8fZXcfL-7OPuXXt4vPZ--vc82hrHKuy7btKgZ1PT_OdCCQKTRdLURddW3TaMY1501ZNkYbqoUpjE44LTraKnaUHe9j18H_nDCO0tmose_VgH6KEnhTiPSHTZHQt_-gKz-FIQ0nGUADwOod9W5P6eBjDGjkOlinwlYClXMryVrKXSuJfXOfOLUOu0fyoYYEnO6BX7bH7f-T5OXt4iEy3xs2jvj70VDhh6wEE6X8drOQH-j5F_H16kYu2F_V-Kfl</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Pongsuthirak, Pallop</creator><creator>Vutyavanich, Teraporn</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TO</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201402</creationdate><title>Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section</title><author>Pongsuthirak, Pallop ; Vutyavanich, Teraporn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4156-4c5bbd631880756fd17e3aefd87786db99c34c449559fcf0c7f2fc31802d0ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Blastocyst - physiology</topic><topic>Cesarean Section</topic><topic>cytoplasmic maturation</topic><topic>Embryo Culture Techniques</topic><topic>Embryonic Development - physiology</topic><topic>Embryos</topic><topic>Female</topic><topic>Fertilization</topic><topic>Fertilization - physiology</topic><topic>Gonadotropins</topic><topic>Humans</topic><topic>in vitro maturation</topic><topic>Maturation</topic><topic>nuclear maturation</topic><topic>Oocyte Retrieval</topic><topic>Oocytes</topic><topic>Pituitary (anterior)</topic><topic>Pregnancy</topic><topic>Sperm Injections, Intracytoplasmic</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pongsuthirak, Pallop</creatorcontrib><creatorcontrib>Vutyavanich, Teraporn</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of obstetrics and gynaecology research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pongsuthirak, Pallop</au><au>Vutyavanich, Teraporn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section</atitle><jtitle>The journal of obstetrics and gynaecology research</jtitle><addtitle>J Obstet Gynaecol Res</addtitle><date>2014-02</date><risdate>2014</risdate><volume>40</volume><issue>2</issue><spage>459</spage><epage>464</epage><pages>459-464</pages><issn>1341-8076</issn><eissn>1447-0756</eissn><abstract>Aim
To investigate the developmental competence of human embryos that originated from in vitro matured oocytes retrieved during cesarean section.
Methods
Immature oocytes were collected from 95 pregnant women, who underwent cesarean section at Buddhachinaraj Hospital Medical School and consented to participate in the study. Retrieved oocytes were cultured in blastocyst medium supplemented with 75 IU/L of human menopausal gonadotropin. Oocyte maturation was assessed at 30 and 48 h after culture. In vitro matured oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 h. The fertilization, cleavage and blastocyst formation rates were observed.
Results
Maturation rate of oocytes after 30 h of culture was 67.9% compared with 13.1% at 48 h (P < 0.0001). Insemination of oocytes in both groups resulted in similar fertilization, cleavage and blastocyst formation rates.
Conclusion
A large proportion of oocytes retrieved at the time of cesarean section exhibited the capacity to undergo maturation in vitro. They can be fertilized and developed into good‐quality blastocyst stage.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>24118307</pmid><doi>10.1111/jog.12186</doi><tpages>6</tpages></addata></record> |
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| ispartof | The journal of obstetrics and gynaecology research, 2014-02, Vol.40 (2), p.459-464 |
| issn | 1341-8076 1447-0756 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adolescent Adult Blastocyst - physiology Cesarean Section cytoplasmic maturation Embryo Culture Techniques Embryonic Development - physiology Embryos Female Fertilization Fertilization - physiology Gonadotropins Humans in vitro maturation Maturation nuclear maturation Oocyte Retrieval Oocytes Pituitary (anterior) Pregnancy Sperm Injections, Intracytoplasmic Young Adult |
| title | Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section |
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