Functional and structural characterization of family 6 carbohydrate-binding module (CtCBM6A) of Clostridium thermocellum α-L-arabinofuranosidase

The gene encoding the family 6 carbohydrate-binding module ( Ct CBM6A) from Clostridium thermocellum , cloned in pET-21a(+) expression vector, was overexpressed using Escherichia coli BL-21(DE3) cells and purified by immobilized metal-ion affinity chromatography. SDS-PAGE analysis of the recombinant...

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Veröffentlicht in:	Biochemistry (Moscow) 2013-11, Vol.78 (11), p.1272-1279
Hauptverfasser:	Ahmed, S., Luís, A. S., Brás, J. L. A., Fontes, C. M. G. A., Goyal, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Binding proteins Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Clostridium Clostridium thermocellum Clostridium thermocellum - enzymology Escherichia coli Escherichia coli - metabolism Genetic aspects Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism Life Sciences Ligands Microbiology Molecular Sequence Data Physiological aspects Protein Binding Protein Denaturation Protein Stability Protein Structure, Tertiary Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Sequence Alignment Triticum - metabolism Triticum aestivum Xylans - chemistry Xylans - metabolism
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