A gold@silica core–shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection
•We successfully synthesized Au nanstar@SiO2 NPs conjugated with the GOx enzyme.•We quantitatively measured glucose level by examining SERS peak of H2O2.•SERS sensing assay showed good anti-interference capability against UA and AA.•This sensor can be used in the real biological matrix such as saliv...
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| Veröffentlicht in: | Analytica chimica acta 2014-02, Vol.811, p.76-80 |
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| container_title | Analytica chimica acta |
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| creator | Al-Ogaidi, Israa Gou, Honglei Al-kazaz, Abdul Kareem A. Aguilar, Zoraida P. Melconian, Alice K. Zheng, Peng Wu, Nianqiang |
| description | •We successfully synthesized Au nanstar@SiO2 NPs conjugated with the GOx enzyme.•We quantitatively measured glucose level by examining SERS peak of H2O2.•SERS sensing assay showed good anti-interference capability against UA and AA.•This sensor can be used in the real biological matrix such as saliva.
The gold nanostar@silica core–shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25μM to 25mM in the aqueous solution with the limit of detection of 16μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample. |
| doi_str_mv | 10.1016/j.aca.2013.12.009 |
| format | Article |
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The gold nanostar@silica core–shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25μM to 25mM in the aqueous solution with the limit of detection of 16μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2013.12.009</identifier><identifier>PMID: 24456597</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Ascorbic Acid - chemistry ; Biosensing Techniques ; Biosensor ; Chemistry Techniques, Analytical - instrumentation ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Gluconates - analysis ; Glucose ; Glucose - analysis ; Glucose Oxidase - chemistry ; Glucose Oxidase - metabolism ; Gold - chemistry ; Hydrogen Peroxide - analysis ; Metal Nanoparticles - chemistry ; Oxidation-Reduction ; Silicon Dioxide - chemistry ; Spectrum Analysis, Raman ; Surface-enhanced Raman scattering (SERS) ; Uric Acid - chemistry</subject><ispartof>Analytica chimica acta, 2014-02, Vol.811, p.76-80</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-201e047ca573357fd789a3d3132cb3388d9966db238f2a2260b376225dd810903</citedby><cites>FETCH-LOGICAL-c353t-201e047ca573357fd789a3d3132cb3388d9966db238f2a2260b376225dd810903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003267013015237$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24456597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Al-Ogaidi, Israa</creatorcontrib><creatorcontrib>Gou, Honglei</creatorcontrib><creatorcontrib>Al-kazaz, Abdul Kareem A.</creatorcontrib><creatorcontrib>Aguilar, Zoraida P.</creatorcontrib><creatorcontrib>Melconian, Alice K.</creatorcontrib><creatorcontrib>Zheng, Peng</creatorcontrib><creatorcontrib>Wu, Nianqiang</creatorcontrib><title>A gold@silica core–shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>•We successfully synthesized Au nanstar@SiO2 NPs conjugated with the GOx enzyme.•We quantitatively measured glucose level by examining SERS peak of H2O2.•SERS sensing assay showed good anti-interference capability against UA and AA.•This sensor can be used in the real biological matrix such as saliva.
The gold nanostar@silica core–shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25μM to 25mM in the aqueous solution with the limit of detection of 16μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample.</description><subject>Ascorbic Acid - chemistry</subject><subject>Biosensing Techniques</subject><subject>Biosensor</subject><subject>Chemistry Techniques, Analytical - instrumentation</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Gluconates - analysis</subject><subject>Glucose</subject><subject>Glucose - analysis</subject><subject>Glucose Oxidase - chemistry</subject><subject>Glucose Oxidase - metabolism</subject><subject>Gold - chemistry</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Oxidation-Reduction</subject><subject>Silicon Dioxide - chemistry</subject><subject>Spectrum Analysis, Raman</subject><subject>Surface-enhanced Raman scattering (SERS)</subject><subject>Uric Acid - chemistry</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9q3DAQxkVpabZpH6CXomMvdvTHtmx6aQhJGwgESnIWY2m80aKVtpJdSE55h75hniRaNs2xh2GYmW8--H6EfOas5ox3J5saDNSCcVlzUTM2vCEr3itZNVI0b8mKMSYr0Sl2RD7kvCmj4Kx5T45E07RdO6gVeTil6-jt9-y8M0BNTPj0-Dffofc0QIg7SLMzHqsRMlqalzSBwQrDHQRTFr9gC4FmA_OMyYU1HV3MGHJMdCrlYURfTQmRrv1iyolanNHMLoaP5N0EPuOnl35Mbi_Ob85-VlfXPy7PTq8qI1s5VyUeskYZaJWUrZqs6geQVnIpzChl39th6Do7CtlPAoTo2ChVJ0Rrbc_ZwOQx-Xrw3aX4e8E8663LpgSEgHHJmjeD6AbV8rZI-UFqUsw54aR3yW0h3WvO9B653uiCXO-Ray50QV5-vrzYL-MW7evHP8ZF8O0gwBLyj8Oks3G4p-dSIaFtdP-xfwbahZLX</recordid><startdate>20140206</startdate><enddate>20140206</enddate><creator>Al-Ogaidi, Israa</creator><creator>Gou, Honglei</creator><creator>Al-kazaz, Abdul Kareem A.</creator><creator>Aguilar, Zoraida P.</creator><creator>Melconian, Alice K.</creator><creator>Zheng, Peng</creator><creator>Wu, Nianqiang</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140206</creationdate><title>A gold@silica core–shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection</title><author>Al-Ogaidi, Israa ; Gou, Honglei ; Al-kazaz, Abdul Kareem A. ; Aguilar, Zoraida P. ; Melconian, Alice K. ; Zheng, Peng ; Wu, Nianqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-201e047ca573357fd789a3d3132cb3388d9966db238f2a2260b376225dd810903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ascorbic Acid - chemistry</topic><topic>Biosensing Techniques</topic><topic>Biosensor</topic><topic>Chemistry Techniques, Analytical - instrumentation</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Gluconates - analysis</topic><topic>Glucose</topic><topic>Glucose - analysis</topic><topic>Glucose Oxidase - chemistry</topic><topic>Glucose Oxidase - metabolism</topic><topic>Gold - chemistry</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Oxidation-Reduction</topic><topic>Silicon Dioxide - chemistry</topic><topic>Spectrum Analysis, Raman</topic><topic>Surface-enhanced Raman scattering (SERS)</topic><topic>Uric Acid - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Al-Ogaidi, Israa</creatorcontrib><creatorcontrib>Gou, Honglei</creatorcontrib><creatorcontrib>Al-kazaz, Abdul Kareem A.</creatorcontrib><creatorcontrib>Aguilar, Zoraida P.</creatorcontrib><creatorcontrib>Melconian, Alice K.</creatorcontrib><creatorcontrib>Zheng, Peng</creatorcontrib><creatorcontrib>Wu, Nianqiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Al-Ogaidi, Israa</au><au>Gou, Honglei</au><au>Al-kazaz, Abdul Kareem A.</au><au>Aguilar, Zoraida P.</au><au>Melconian, Alice K.</au><au>Zheng, Peng</au><au>Wu, Nianqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A gold@silica core–shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2014-02-06</date><risdate>2014</risdate><volume>811</volume><spage>76</spage><epage>80</epage><pages>76-80</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><abstract>•We successfully synthesized Au nanstar@SiO2 NPs conjugated with the GOx enzyme.•We quantitatively measured glucose level by examining SERS peak of H2O2.•SERS sensing assay showed good anti-interference capability against UA and AA.•This sensor can be used in the real biological matrix such as saliva.
The gold nanostar@silica core–shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25μM to 25mM in the aqueous solution with the limit of detection of 16μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24456597</pmid><doi>10.1016/j.aca.2013.12.009</doi><tpages>5</tpages></addata></record> |
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| subjects | Ascorbic Acid - chemistry Biosensing Techniques Biosensor Chemistry Techniques, Analytical - instrumentation Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Gluconates - analysis Glucose Glucose - analysis Glucose Oxidase - chemistry Glucose Oxidase - metabolism Gold - chemistry Hydrogen Peroxide - analysis Metal Nanoparticles - chemistry Oxidation-Reduction Silicon Dioxide - chemistry Spectrum Analysis, Raman Surface-enhanced Raman scattering (SERS) Uric Acid - chemistry |
| title | A gold@silica core–shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection |
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