ZBTB20 is involved in liver regeneration after partial hepatectomy in mouse
BACKGROUND: A better understanding of the molecular mechanisms in liver regeneration holds promise for exploring the new potential therapy for liver failure. The present study was to investigate the role of zinc finger and BTB domain-containing protein 20(ZBTB20), a potential factor associated with...
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| Veröffentlicht in: | Hepatobiliary & pancreatic diseases international 2014-02, Vol.13 (1), p.48-54 |
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| container_title | Hepatobiliary & pancreatic diseases international |
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| creator | Weng, Ming-Zhe Zhuang, Peng-Yuan Hei, Zhen-Yu Lin, Pei-Yi Chen, Zhi-Sheng Liu, Ying-Bin Quan, Zhi-Wei Tang, Zhao-Hui |
| description | BACKGROUND: A better understanding of the molecular mechanisms in liver regeneration holds promise for exploring the new potential therapy for liver failure. The present study was to investigate the role of zinc finger and BTB domain-containing protein 20(ZBTB20), a potential factor associated with liver regeneration, in a model of 70% hepatectomy in mice. METHODS: Parameters for liver proliferation such as liver/body ratio and BrdU positivity were obtained via direct measurement and immunohistochemistry. The levels of zinc fingers and homeoboxes 2(ZHX2), ZBTB20, alpha-fetoprotein(AFP) and glypican 3(GPC3) transcripts in the regenerating liver tissue of a 70% hepatectomy rodent model were monitored by real-time PCR analysis at different time points. Knockdown of ZBTB20 was performed to characterize its regulatory function. RESULTS: A negatively regulating relationship between ZHX2, ZBTB20 and AFP, GPC3 was revealed from 24 to 72 hours after 70% hepatectomy. ZBTB20 appears to negatively regulate AFP and GPC3 transcription since the knockdown of ZBTB20 promoted the proliferation of hepatocytes and the expression of AFP and GPC3. CONCLUSION: In addition to AFP, GPC3 and ZHX2, ZBTB20 is a new regulator in liver regeneration and the decrease of ZBTB20 expression following 70% hepatectomy promotes AFP and GPC3 expression. |
| doi_str_mv | 10.1016/S1499-3872(14)60006-0 |
| format | Article |
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The present study was to investigate the role of zinc finger and BTB domain-containing protein 20(ZBTB20), a potential factor associated with liver regeneration, in a model of 70% hepatectomy in mice. METHODS: Parameters for liver proliferation such as liver/body ratio and BrdU positivity were obtained via direct measurement and immunohistochemistry. The levels of zinc fingers and homeoboxes 2(ZHX2), ZBTB20, alpha-fetoprotein(AFP) and glypican 3(GPC3) transcripts in the regenerating liver tissue of a 70% hepatectomy rodent model were monitored by real-time PCR analysis at different time points. Knockdown of ZBTB20 was performed to characterize its regulatory function. RESULTS: A negatively regulating relationship between ZHX2, ZBTB20 and AFP, GPC3 was revealed from 24 to 72 hours after 70% hepatectomy. ZBTB20 appears to negatively regulate AFP and GPC3 transcription since the knockdown of ZBTB20 promoted the proliferation of hepatocytes and the expression of AFP and GPC3. CONCLUSION: In addition to AFP, GPC3 and ZHX2, ZBTB20 is a new regulator in liver regeneration and the decrease of ZBTB20 expression following 70% hepatectomy promotes AFP and GPC3 expression.</description><identifier>ISSN: 1499-3872</identifier><identifier>DOI: 10.1016/S1499-3872(14)60006-0</identifier><identifier>PMID: 24463079</identifier><language>eng</language><publisher>Singapore: Elsevier B.V</publisher><subject>alpha-Fetoproteins - physiology ; Animals ; Cell Line ; Cell Proliferation ; Endocrinology & Metabolism ; Gastroenterology and Hepatology ; Glypicans - physiology ; hepatectomy ; Hepatectomy - methods ; Hepatocytes - pathology ; Homeodomain Proteins - physiology ; liver ; Liver - physiology ; Liver - surgery ; liver regeneration ; Liver Regeneration - physiology ; Mice ; Mice, Inbred C57BL ; Models, Animal ; mouse ; partial ; partial hepatectomy ; regeneration ; RNA, Small Interfering - pharmacology ; Time Factors ; Transcription Factors - drug effects ; Transcription Factors - genetics ; Transcription Factors - physiology ; Transfection ; ZBTB20</subject><ispartof>Hepatobiliary & pancreatic diseases international, 2014-02, Vol.13 (1), p.48-54</ispartof><rights>The Editorial Board of Hepatobiliary & Pancreatic Diseases International</rights><rights>2014 The Editorial Board of Hepatobiliary & Pancreatic Diseases International</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-5a9a252be437683381319a6649fd4a8d4fd4fc83253d54cc4d670112375f22123</citedby><cites>FETCH-LOGICAL-c500t-5a9a252be437683381319a6649fd4a8d4fd4fc83253d54cc4d670112375f22123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/89801X/89801X.jpg</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1499387214600060$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24463079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weng, Ming-Zhe</creatorcontrib><creatorcontrib>Zhuang, Peng-Yuan</creatorcontrib><creatorcontrib>Hei, Zhen-Yu</creatorcontrib><creatorcontrib>Lin, Pei-Yi</creatorcontrib><creatorcontrib>Chen, Zhi-Sheng</creatorcontrib><creatorcontrib>Liu, Ying-Bin</creatorcontrib><creatorcontrib>Quan, Zhi-Wei</creatorcontrib><creatorcontrib>Tang, Zhao-Hui</creatorcontrib><title>ZBTB20 is involved in liver regeneration after partial hepatectomy in mouse</title><title>Hepatobiliary & pancreatic diseases international</title><addtitle>Hepatobiliary & Pancreatic Diseases International</addtitle><description>BACKGROUND: A better understanding of the molecular mechanisms in liver regeneration holds promise for exploring the new potential therapy for liver failure. The present study was to investigate the role of zinc finger and BTB domain-containing protein 20(ZBTB20), a potential factor associated with liver regeneration, in a model of 70% hepatectomy in mice. METHODS: Parameters for liver proliferation such as liver/body ratio and BrdU positivity were obtained via direct measurement and immunohistochemistry. The levels of zinc fingers and homeoboxes 2(ZHX2), ZBTB20, alpha-fetoprotein(AFP) and glypican 3(GPC3) transcripts in the regenerating liver tissue of a 70% hepatectomy rodent model were monitored by real-time PCR analysis at different time points. Knockdown of ZBTB20 was performed to characterize its regulatory function. RESULTS: A negatively regulating relationship between ZHX2, ZBTB20 and AFP, GPC3 was revealed from 24 to 72 hours after 70% hepatectomy. ZBTB20 appears to negatively regulate AFP and GPC3 transcription since the knockdown of ZBTB20 promoted the proliferation of hepatocytes and the expression of AFP and GPC3. CONCLUSION: In addition to AFP, GPC3 and ZHX2, ZBTB20 is a new regulator in liver regeneration and the decrease of ZBTB20 expression following 70% hepatectomy promotes AFP and GPC3 expression.</description><subject>alpha-Fetoproteins - physiology</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Endocrinology & Metabolism</subject><subject>Gastroenterology and Hepatology</subject><subject>Glypicans - physiology</subject><subject>hepatectomy</subject><subject>Hepatectomy - methods</subject><subject>Hepatocytes - pathology</subject><subject>Homeodomain Proteins - physiology</subject><subject>liver</subject><subject>Liver - physiology</subject><subject>Liver - surgery</subject><subject>liver regeneration</subject><subject>Liver Regeneration - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Models, Animal</subject><subject>mouse</subject><subject>partial</subject><subject>partial hepatectomy</subject><subject>regeneration</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>Time Factors</subject><subject>Transcription Factors - drug effects</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><subject>Transfection</subject><subject>ZBTB20</subject><issn>1499-3872</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9v1DAQxX0AtaXwEUCRuJRDYPw3zgXUVi2tqMSBcuFiuc5k65LYWzu70n57nN2lQlx6etbozRu_HyFvKXykQNWnH1S0bc11w06o-KAAQNXwghw9jQ_Jq5wfAJjWUh2QQyaE4tC0R-Tbr7PbMwaVz5UP6zissSuPavBrTFXCBQZMdvIxVLafymhp0-TtUN3j0k7opjhuZv8YVxlfk5e9HTK-2esx-Xl5cXt-Vd98_3p9fnpTOwkw1dK2lkl2h4I3SnOuKaetVUq0fSes7kSR3mnOJO-kcE50qgFKGW9kz1jRY3Kyy12m-LjCPJnRZ4fDYAOWf5hSmymtJGuLVe6sLsWcE_Zmmfxo08ZQMDM7s2VnZkhlz2zZGSh77_YnVncjdk9bf8EVw5edAUvRtcdksvMYHHY-FSymi_7ZE5__S3CDD97Z4TduMD_EVQqFoqEmMwO7kDmDim3CHPB-3-0-hsWjD4t_ygEHLoQG_gdbeZ7I</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Weng, Ming-Zhe</creator><creator>Zhuang, Peng-Yuan</creator><creator>Hei, Zhen-Yu</creator><creator>Lin, Pei-Yi</creator><creator>Chen, Zhi-Sheng</creator><creator>Liu, Ying-Bin</creator><creator>Quan, Zhi-Wei</creator><creator>Tang, Zhao-Hui</creator><general>Elsevier B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201402</creationdate><title>ZBTB20 is involved in liver regeneration after partial hepatectomy in mouse</title><author>Weng, Ming-Zhe ; Zhuang, Peng-Yuan ; Hei, Zhen-Yu ; Lin, Pei-Yi ; Chen, Zhi-Sheng ; Liu, Ying-Bin ; Quan, Zhi-Wei ; Tang, Zhao-Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-5a9a252be437683381319a6649fd4a8d4fd4fc83253d54cc4d670112375f22123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>alpha-Fetoproteins - physiology</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Endocrinology & Metabolism</topic><topic>Gastroenterology and Hepatology</topic><topic>Glypicans - physiology</topic><topic>hepatectomy</topic><topic>Hepatectomy - methods</topic><topic>Hepatocytes - pathology</topic><topic>Homeodomain Proteins - physiology</topic><topic>liver</topic><topic>Liver - physiology</topic><topic>Liver - surgery</topic><topic>liver regeneration</topic><topic>Liver Regeneration - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Models, Animal</topic><topic>mouse</topic><topic>partial</topic><topic>partial hepatectomy</topic><topic>regeneration</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>Time Factors</topic><topic>Transcription Factors - drug effects</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><topic>Transfection</topic><topic>ZBTB20</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weng, Ming-Zhe</creatorcontrib><creatorcontrib>Zhuang, Peng-Yuan</creatorcontrib><creatorcontrib>Hei, Zhen-Yu</creatorcontrib><creatorcontrib>Lin, Pei-Yi</creatorcontrib><creatorcontrib>Chen, Zhi-Sheng</creatorcontrib><creatorcontrib>Liu, Ying-Bin</creatorcontrib><creatorcontrib>Quan, Zhi-Wei</creatorcontrib><creatorcontrib>Tang, Zhao-Hui</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hepatobiliary & pancreatic diseases international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weng, Ming-Zhe</au><au>Zhuang, Peng-Yuan</au><au>Hei, Zhen-Yu</au><au>Lin, Pei-Yi</au><au>Chen, Zhi-Sheng</au><au>Liu, Ying-Bin</au><au>Quan, Zhi-Wei</au><au>Tang, Zhao-Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ZBTB20 is involved in liver regeneration after partial hepatectomy in mouse</atitle><jtitle>Hepatobiliary & pancreatic diseases international</jtitle><addtitle>Hepatobiliary & Pancreatic Diseases International</addtitle><date>2014-02</date><risdate>2014</risdate><volume>13</volume><issue>1</issue><spage>48</spage><epage>54</epage><pages>48-54</pages><issn>1499-3872</issn><abstract>BACKGROUND: A better understanding of the molecular mechanisms in liver regeneration holds promise for exploring the new potential therapy for liver failure. The present study was to investigate the role of zinc finger and BTB domain-containing protein 20(ZBTB20), a potential factor associated with liver regeneration, in a model of 70% hepatectomy in mice. METHODS: Parameters for liver proliferation such as liver/body ratio and BrdU positivity were obtained via direct measurement and immunohistochemistry. The levels of zinc fingers and homeoboxes 2(ZHX2), ZBTB20, alpha-fetoprotein(AFP) and glypican 3(GPC3) transcripts in the regenerating liver tissue of a 70% hepatectomy rodent model were monitored by real-time PCR analysis at different time points. Knockdown of ZBTB20 was performed to characterize its regulatory function. RESULTS: A negatively regulating relationship between ZHX2, ZBTB20 and AFP, GPC3 was revealed from 24 to 72 hours after 70% hepatectomy. ZBTB20 appears to negatively regulate AFP and GPC3 transcription since the knockdown of ZBTB20 promoted the proliferation of hepatocytes and the expression of AFP and GPC3. CONCLUSION: In addition to AFP, GPC3 and ZHX2, ZBTB20 is a new regulator in liver regeneration and the decrease of ZBTB20 expression following 70% hepatectomy promotes AFP and GPC3 expression.</abstract><cop>Singapore</cop><pub>Elsevier B.V</pub><pmid>24463079</pmid><doi>10.1016/S1499-3872(14)60006-0</doi><tpages>7</tpages></addata></record> |
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| subjects | alpha-Fetoproteins - physiology Animals Cell Line Cell Proliferation Endocrinology & Metabolism Gastroenterology and Hepatology Glypicans - physiology hepatectomy Hepatectomy - methods Hepatocytes - pathology Homeodomain Proteins - physiology liver Liver - physiology Liver - surgery liver regeneration Liver Regeneration - physiology Mice Mice, Inbred C57BL Models, Animal mouse partial partial hepatectomy regeneration RNA, Small Interfering - pharmacology Time Factors Transcription Factors - drug effects Transcription Factors - genetics Transcription Factors - physiology Transfection ZBTB20 |
| title | ZBTB20 is involved in liver regeneration after partial hepatectomy in mouse |
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