DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA

Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiol-labeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconj...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	ACS applied materials & interfaces 2014-01, Vol.6 (2), p.1152-1157
Hauptverfasser:	Su, Shao, Fan, Jinwei, Xue, Bing, Yuwen, Lihui, Liu, Xingfen, Pan, Dun, Fan, Chunhai, Wang, Lianhui
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques DNA - chemistry DNA - isolation & purification Fluorescence Fluorescence Resonance Energy Transfer MicroRNAs - chemistry MicroRNAs - isolation & purification Quantum Dots - chemistry Water - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	1157
container_issue	2
container_start_page	1152
container_title	ACS applied materials & interfaces
container_volume	6
creator	Su, Shao Fan, Jinwei Xue, Bing Yuwen, Lihui Liu, Xingfen Pan, Dun Fan, Chunhai Wang, Lianhui
description	Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiol-labeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ2) via Förster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis.
doi_str_mv	10.1021/am404811j
format	Article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1492680939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1492680939</sourcerecordid><originalsourceid>FETCH-LOGICAL-a315t-16bd95eb866dd4f42970f5445452f7b7a98d835960f3bde205a56b3cd75cab5c3</originalsourceid><addsrcrecordid>eNptkL1OwzAYRS0EoqUw8ALICxIMAf8nGauWUqSqiL85cmKnJGrsYjtIfXuMWjoxfd9wdHTvBeASozuMCL6XHUMsw7g9AkOcM5ZkhJPjw8_YAJx53yIkKEH8FAwIoxmigg-Bmi7HycSatl_JoBV86aUJfQenNsClNHbjbKlhbR2cN6vP5E0b34TmuwlbOFv31mlfaRPgVAddhcYaaGsYlVAaBbumcjZ5XY7PwUkt115f7O8IfMwe3ifzZPH8-DQZLxJJMQ8JFqXKuS4zIZRiNSN5imrOGGec1GmZyjxTGeW5QDUtlY5VJBclrVTKK1nyio7Azc4bU3_12oeia2K-9VoabXtfYJYTkaGc5hG93aExovdO18XGNZ102wKj4nfU4jBqZK_22r7stDqQfytG4HoHyMoXre2diS3_Ef0AVGp87A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1492680939</pqid></control><display><type>article</type><title>DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Su, Shao ; Fan, Jinwei ; Xue, Bing ; Yuwen, Lihui ; Liu, Xingfen ; Pan, Dun ; Fan, Chunhai ; Wang, Lianhui</creator><creatorcontrib>Su, Shao ; Fan, Jinwei ; Xue, Bing ; Yuwen, Lihui ; Liu, Xingfen ; Pan, Dun ; Fan, Chunhai ; Wang, Lianhui</creatorcontrib><description>Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiol-labeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ2) via Förster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/am404811j</identifier><identifier>PMID: 24380365</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biosensing Techniques ; DNA - chemistry ; DNA - isolation & purification ; Fluorescence ; Fluorescence Resonance Energy Transfer ; MicroRNAs - chemistry ; MicroRNAs - isolation & purification ; Quantum Dots - chemistry ; Water - chemistry</subject><ispartof>ACS applied materials & interfaces, 2014-01, Vol.6 (2), p.1152-1157</ispartof><rights>Copyright © 2013 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a315t-16bd95eb866dd4f42970f5445452f7b7a98d835960f3bde205a56b3cd75cab5c3</citedby><cites>FETCH-LOGICAL-a315t-16bd95eb866dd4f42970f5445452f7b7a98d835960f3bde205a56b3cd75cab5c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/am404811j$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/am404811j$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24380365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Shao</creatorcontrib><creatorcontrib>Fan, Jinwei</creatorcontrib><creatorcontrib>Xue, Bing</creatorcontrib><creatorcontrib>Yuwen, Lihui</creatorcontrib><creatorcontrib>Liu, Xingfen</creatorcontrib><creatorcontrib>Pan, Dun</creatorcontrib><creatorcontrib>Fan, Chunhai</creatorcontrib><creatorcontrib>Wang, Lianhui</creatorcontrib><title>DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiol-labeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ2) via Förster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis.</description><subject>Biosensing Techniques</subject><subject>DNA - chemistry</subject><subject>DNA - isolation & purification</subject><subject>Fluorescence</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>MicroRNAs - chemistry</subject><subject>MicroRNAs - isolation & purification</subject><subject>Quantum Dots - chemistry</subject><subject>Water - chemistry</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkL1OwzAYRS0EoqUw8ALICxIMAf8nGauWUqSqiL85cmKnJGrsYjtIfXuMWjoxfd9wdHTvBeASozuMCL6XHUMsw7g9AkOcM5ZkhJPjw8_YAJx53yIkKEH8FAwIoxmigg-Bmi7HycSatl_JoBV86aUJfQenNsClNHbjbKlhbR2cN6vP5E0b34TmuwlbOFv31mlfaRPgVAddhcYaaGsYlVAaBbumcjZ5XY7PwUkt115f7O8IfMwe3ifzZPH8-DQZLxJJMQ8JFqXKuS4zIZRiNSN5imrOGGec1GmZyjxTGeW5QDUtlY5VJBclrVTKK1nyio7Azc4bU3_12oeia2K-9VoabXtfYJYTkaGc5hG93aExovdO18XGNZ102wKj4nfU4jBqZK_22r7stDqQfytG4HoHyMoXre2diS3_Ef0AVGp87A</recordid><startdate>20140122</startdate><enddate>20140122</enddate><creator>Su, Shao</creator><creator>Fan, Jinwei</creator><creator>Xue, Bing</creator><creator>Yuwen, Lihui</creator><creator>Liu, Xingfen</creator><creator>Pan, Dun</creator><creator>Fan, Chunhai</creator><creator>Wang, Lianhui</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140122</creationdate><title>DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA</title><author>Su, Shao ; Fan, Jinwei ; Xue, Bing ; Yuwen, Lihui ; Liu, Xingfen ; Pan, Dun ; Fan, Chunhai ; Wang, Lianhui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a315t-16bd95eb866dd4f42970f5445452f7b7a98d835960f3bde205a56b3cd75cab5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biosensing Techniques</topic><topic>DNA - chemistry</topic><topic>DNA - isolation & purification</topic><topic>Fluorescence</topic><topic>Fluorescence Resonance Energy Transfer</topic><topic>MicroRNAs - chemistry</topic><topic>MicroRNAs - isolation & purification</topic><topic>Quantum Dots - chemistry</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Shao</creatorcontrib><creatorcontrib>Fan, Jinwei</creatorcontrib><creatorcontrib>Xue, Bing</creatorcontrib><creatorcontrib>Yuwen, Lihui</creatorcontrib><creatorcontrib>Liu, Xingfen</creatorcontrib><creatorcontrib>Pan, Dun</creatorcontrib><creatorcontrib>Fan, Chunhai</creatorcontrib><creatorcontrib>Wang, Lianhui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Shao</au><au>Fan, Jinwei</au><au>Xue, Bing</au><au>Yuwen, Lihui</au><au>Liu, Xingfen</au><au>Pan, Dun</au><au>Fan, Chunhai</au><au>Wang, Lianhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2014-01-22</date><risdate>2014</risdate><volume>6</volume><issue>2</issue><spage>1152</spage><epage>1157</epage><pages>1152-1157</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiol-labeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ2) via Förster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24380365</pmid><doi>10.1021/am404811j</doi><tpages>6</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 1944-8244
ispartof	ACS applied materials & interfaces, 2014-01, Vol.6 (2), p.1152-1157
issn	1944-8244 1944-8252
language	eng
recordid	cdi_proquest_miscellaneous_1492680939
source	MEDLINE; American Chemical Society Journals
subjects	Biosensing Techniques DNA - chemistry DNA - isolation & purification Fluorescence Fluorescence Resonance Energy Transfer MicroRNAs - chemistry MicroRNAs - isolation & purification Quantum Dots - chemistry Water - chemistry
title	DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T06%3A05%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA-Conjugated%20Quantum%20Dot%20Nanoprobe%20for%20High-Sensitivity%20Fluorescent%20Detection%20of%20DNA%20and%20micro-RNA&rft.jtitle=ACS%20applied%20materials%20&%20interfaces&rft.au=Su,%20Shao&rft.date=2014-01-22&rft.volume=6&rft.issue=2&rft.spage=1152&rft.epage=1157&rft.pages=1152-1157&rft.issn=1944-8244&rft.eissn=1944-8252&rft_id=info:doi/10.1021/am404811j&rft_dat=%3Cproquest_cross%3E1492680939%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1492680939&rft_id=info:pmid/24380365&rfr_iscdi=true