Determination of binding curves via protein micropatterning in vitro and in living cells

Quantification of protein interactions in living cells is of key relevance for understanding cellular signaling. With current techniques, however, it is difficult to determine binding affinities and stoichiometries of protein complexes in the plasma membrane. We introduce here protein micropatternin...

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Veröffentlicht in:Cytometry. Part A 2013-09, Vol.83 (9), p.847-854
Hauptverfasser: Sunzenauer, Stefan, Zojer, Verena, Brameshuber, Mario, Tröls, Andreas, Weghuber, Julian, Stockinger, Hannes, Schütz, Gerhard J.
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Sprache:eng
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