Effect of enzyme-assisted extract of Sargassum coreanum on induction of apoptosis in HL-60 tumor cells

In order to effectively prepare useful components from Sargassum coreanum, enzyme-assisted extraction was adapted to extraction processing of the seaweed. Our previous studies have shown that Neutrase extract of S. coreanum exhibited the strongest antioxidant activities among ten enzymatic extracts....

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Veröffentlicht in:Journal of applied phycology 2012-08, Vol.24 (4), p.675-684
Hauptverfasser: Ko, Seok-Chun, Lee, Seung-Hong, Ahn, Ginnae, Kim, Kil-Nam, Cha, Seon-Heui, Kim, Se-Kwon, Jeon, Byong-Tae, Park, Pyo-Jam, Lee, Ki-Wan, Jeon, You-Jin
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container_issue 4
container_start_page 675
container_title Journal of applied phycology
container_volume 24
creator Ko, Seok-Chun
Lee, Seung-Hong
Ahn, Ginnae
Kim, Kil-Nam
Cha, Seon-Heui
Kim, Se-Kwon
Jeon, Byong-Tae
Park, Pyo-Jam
Lee, Ki-Wan
Jeon, You-Jin
description In order to effectively prepare useful components from Sargassum coreanum, enzyme-assisted extraction was adapted to extraction processing of the seaweed. Our previous studies have shown that Neutrase extract of S. coreanum exhibited the strongest antioxidant activities among ten enzymatic extracts. It is reported that many antioxidant substances have anticancer or anticarcinogenic properties. Thus, Neutrase extract, which possessed a strong antioxidant activity in the previous study, was separated into four different molecular weight fractions (30 kDa) which were screened for inhibitory activities against cancer cell growth. The >30-kDa fraction inhibited cell growth more than the other fractions. We separated crude polysaccharide (CPS) from the >30-kDa fraction to show that CPS increased DNA fragmentation, apoptotic body, and apoptotic cells with hypodiploid DNA contents in HL-60 cells. This indicates that CPS suppressed the growth of cells through apoptosis. The CPS gradually increased the expression of pro-apoptotic Bax and led to the activation of caspase-3 and cleavage of poly(ADP-ribose) polymerase. The CPS was 40.85% fucose, 27.24% galactose, and 12.99% glucose, as analyzed by bio-liquid chromatography. These findings suggest that apoptosis effect can be mediated by CPS in HL-60 cells.
doi_str_mv 10.1007/s10811-011-9685-0
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The CPS gradually increased the expression of pro-apoptotic Bax and led to the activation of caspase-3 and cleavage of poly(ADP-ribose) polymerase. The CPS was 40.85% fucose, 27.24% galactose, and 12.99% glucose, as analyzed by bio-liquid chromatography. 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source SpringerNature Journals
subjects anticarcinogenic activity
antioxidant activity
apoptosis
Biomedical and Life Sciences
caspase-3
cell growth
DNA fragmentation
Ecology
Freshwater & Marine Ecology
fucose
galactose
glucose
Life Sciences
molecular weight
Plant Physiology
Plant Sciences
Sargassum
title Effect of enzyme-assisted extract of Sargassum coreanum on induction of apoptosis in HL-60 tumor cells
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