Flow‐cytometric analysis of rare antigen‐specific T cells
The cytometric enumeration and characterization of antigen‐specific lymphocytes, as introduced about 15 years ago, has contributed significantly to our understanding of adaptive immune responses in health and disease. Despite the development of several technologies, allowing to directly or indirectl...
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| Veröffentlicht in: | Cytometry. Part A 2013-08, Vol.83A (8), p.692-701 |
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| container_title | Cytometry. Part A |
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| creator | Bacher, Petra Scheffold, Alexander |
| description | The cytometric enumeration and characterization of antigen‐specific lymphocytes, as introduced about 15 years ago, has contributed significantly to our understanding of adaptive immune responses in health and disease. Despite the development of several technologies, allowing to directly or indirectly analyze many aspects of lymphocyte specificity and function, several unresolved issues remain, due to the low frequency of certain antigen‐specific lymphocyte subsets and the complexity of T cell antigen recognition. This is especially true for CD4+ conventional as well as regulatory T cells, which bring major contributions to immune protection and pathology. Here we review the current technologies for the analysis of antigen specific T cells within the physiologic T cell repertoire and with a special focus on recent technologies addressing the analysis of rare antigen‐specific T cell populations including naive and regulatory T cells. © 2013 International Society for Advancement of Cytometry |
| doi_str_mv | 10.1002/cyto.a.22317 |
| format | Article |
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| ispartof | Cytometry. Part A, 2013-08, Vol.83A (8), p.692-701 |
| issn | 1552-4922 1552-4930 |
| language | eng |
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| source | MEDLINE; Wiley Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Animals Antigens, CD - metabolism antigen‐specific T cells CD137 CD154 CD4 antigen Cell Separation cytokine secretion assay Flow Cytometry - methods flow‐cytometry Humans Lymphocyte Activation MACS MHC multimer rare cells Receptors, Antigen, T-Cell - metabolism T-Lymphocytes, Regulatory - cytology T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism tetramer |
| title | Flow‐cytometric analysis of rare antigen‐specific T cells |
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