Detection of cytoplasmic nucleophosmin expression by imaging flow cytometry
Mutations within the nucleophosmin NPM1 gene occur in approximately one‐third of cases of acute myeloid leukemia (AML). These mutations result in cytoplasmic accumulation of the mutant NPM protein. NPM1 mutations are currently detected by molecular methods. Using samples from 37 AML patients, we inv...
Gespeichert in:
Veröffentlicht in: | Cytometry. Part A 2012-10, Vol.81A (10), p.896-900 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 900 |
---|---|
container_issue | 10 |
container_start_page | 896 |
container_title | Cytometry. Part A |
container_volume | 81A |
creator | Grimwade, Lizz Gudgin, Emma Bloxham, David Bottley, Graham Vassiliou, George Huntly, Brian Scott, Mike A. Erber, Wendy N. |
description | Mutations within the nucleophosmin NPM1 gene occur in approximately one‐third of cases of acute myeloid leukemia (AML). These mutations result in cytoplasmic accumulation of the mutant NPM protein. NPM1 mutations are currently detected by molecular methods. Using samples from 37 AML patients, we investigated whether imaging flow cytometry could be a viable alternative to this current technique. Bone marrow/peripheral blood cells were stained with anti‐NPM antibody and DRAQ5 nuclear stain, and data were acquired on an ImageStream imaging flow cytometer (Amnis Corp., Seattle, USA). Using the similarity feature for data analysis, we demonstrated that this technique could successfully identify cases of AML with a NPM1 mutation based on cytoplasmic NPM protein staining (at similarity threshold of 1.1 sensitivity 88% and specificity 90%). Combining data of mean fluorescence intensity and % dissimilar staining in a 0–2 scoring system further improved the sensitivity (100%). Imaging flow cytometry has the potential to be included as part of a standard flow cytometry antibody panel to identify potential NPM1 mutations as part of diagnosis and minimal residual disease monitoring. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of hematological malignancies, including the potential to integrate modalities. © 2012 International Society for Advancement of Cytometry. |
doi_str_mv | 10.1002/cyto.a.22116 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1492644066</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1069210886</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4726-4a071bc6f6269ad1b2e8a68c977b962bc503cafe2a525de3814d3e9a5def56073</originalsourceid><addsrcrecordid>eNqFkM9PgzAUxxujcXN682w4epDZPqDQo5k_45Jd5sETKeUxMUCRQib_vWXMHfXU1-TzPnnfLyGXjM4ZpXCr-lbP5RyAMX5EpiwIwPWFR48PM8CEnBnzSakXUA9OyQRA8EhwPiWv99iianNdOTpzBlddSFPmyqk6VaCuP7T9VQ5-1w0aM3BJ7-Sl3OTVxskKvd0tldg2_Tk5yWRh8GL_zsjb48N68ewuV08vi7ulq_wQuOtLGrJE8YwDFzJlCWAkeaREGCaCQ6LskUpmCDKAIEUvYn7qoZB2zgJOQ29Grkdv3eivDk0bl7lRWBSyQt2ZmNnI3Pcp5_-jlAtgNIoG9GZEVaONaTCL68bmbHoLxUPT8RA0lvGuaYtf7c1dUmJ6gH-rtYA3Atu8wP5PWbx4X69G7Q8hA4s7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1069210886</pqid></control><display><type>article</type><title>Detection of cytoplasmic nucleophosmin expression by imaging flow cytometry</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley Free Content</source><source>Alma/SFX Local Collection</source><creator>Grimwade, Lizz ; Gudgin, Emma ; Bloxham, David ; Bottley, Graham ; Vassiliou, George ; Huntly, Brian ; Scott, Mike A. ; Erber, Wendy N.</creator><creatorcontrib>Grimwade, Lizz ; Gudgin, Emma ; Bloxham, David ; Bottley, Graham ; Vassiliou, George ; Huntly, Brian ; Scott, Mike A. ; Erber, Wendy N.</creatorcontrib><description>Mutations within the nucleophosmin NPM1 gene occur in approximately one‐third of cases of acute myeloid leukemia (AML). These mutations result in cytoplasmic accumulation of the mutant NPM protein. NPM1 mutations are currently detected by molecular methods. Using samples from 37 AML patients, we investigated whether imaging flow cytometry could be a viable alternative to this current technique. Bone marrow/peripheral blood cells were stained with anti‐NPM antibody and DRAQ5 nuclear stain, and data were acquired on an ImageStream imaging flow cytometer (Amnis Corp., Seattle, USA). Using the similarity feature for data analysis, we demonstrated that this technique could successfully identify cases of AML with a NPM1 mutation based on cytoplasmic NPM protein staining (at similarity threshold of 1.1 sensitivity 88% and specificity 90%). Combining data of mean fluorescence intensity and % dissimilar staining in a 0–2 scoring system further improved the sensitivity (100%). Imaging flow cytometry has the potential to be included as part of a standard flow cytometry antibody panel to identify potential NPM1 mutations as part of diagnosis and minimal residual disease monitoring. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of hematological malignancies, including the potential to integrate modalities. © 2012 International Society for Advancement of Cytometry.</description><identifier>ISSN: 1552-4922</identifier><identifier>EISSN: 1552-4930</identifier><identifier>DOI: 10.1002/cyto.a.22116</identifier><identifier>PMID: 22968966</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>acute leukemia ; Acute myeloid leukemia ; Adult ; Aged ; Aged, 80 and over ; Anthraquinones ; Antibodies ; Bone Marrow - metabolism ; Bone Marrow - pathology ; Cell Nucleus - genetics ; Cell Nucleus - metabolism ; Cytoplasm - genetics ; Cytoplasm - metabolism ; Female ; Flow Cytometry ; Gene Expression ; hematology ; Humans ; imaging flow cytometry ; Leukemia, Myeloid, Acute - diagnosis ; Leukemia, Myeloid, Acute - genetics ; Leukemia, Myeloid, Acute - metabolism ; Leukocytes, Mononuclear - metabolism ; Leukocytes, Mononuclear - pathology ; Male ; Middle Aged ; Mutation ; Neoplasm, Residual - diagnosis ; Neoplasm, Residual - genetics ; Neoplasm, Residual - metabolism ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; nucleophosmin ; Research Design ; Sensitivity and Specificity ; Staining and Labeling</subject><ispartof>Cytometry. Part A, 2012-10, Vol.81A (10), p.896-900</ispartof><rights>Copyright © 2012 International Society for Advancement of Cytometry</rights><rights>Copyright © 2012 International Society for Advancement of Cytometry.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4726-4a071bc6f6269ad1b2e8a68c977b962bc503cafe2a525de3814d3e9a5def56073</citedby><cites>FETCH-LOGICAL-c4726-4a071bc6f6269ad1b2e8a68c977b962bc503cafe2a525de3814d3e9a5def56073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcyto.a.22116$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcyto.a.22116$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22968966$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grimwade, Lizz</creatorcontrib><creatorcontrib>Gudgin, Emma</creatorcontrib><creatorcontrib>Bloxham, David</creatorcontrib><creatorcontrib>Bottley, Graham</creatorcontrib><creatorcontrib>Vassiliou, George</creatorcontrib><creatorcontrib>Huntly, Brian</creatorcontrib><creatorcontrib>Scott, Mike A.</creatorcontrib><creatorcontrib>Erber, Wendy N.</creatorcontrib><title>Detection of cytoplasmic nucleophosmin expression by imaging flow cytometry</title><title>Cytometry. Part A</title><addtitle>Cytometry A</addtitle><description>Mutations within the nucleophosmin NPM1 gene occur in approximately one‐third of cases of acute myeloid leukemia (AML). These mutations result in cytoplasmic accumulation of the mutant NPM protein. NPM1 mutations are currently detected by molecular methods. Using samples from 37 AML patients, we investigated whether imaging flow cytometry could be a viable alternative to this current technique. Bone marrow/peripheral blood cells were stained with anti‐NPM antibody and DRAQ5 nuclear stain, and data were acquired on an ImageStream imaging flow cytometer (Amnis Corp., Seattle, USA). Using the similarity feature for data analysis, we demonstrated that this technique could successfully identify cases of AML with a NPM1 mutation based on cytoplasmic NPM protein staining (at similarity threshold of 1.1 sensitivity 88% and specificity 90%). Combining data of mean fluorescence intensity and % dissimilar staining in a 0–2 scoring system further improved the sensitivity (100%). Imaging flow cytometry has the potential to be included as part of a standard flow cytometry antibody panel to identify potential NPM1 mutations as part of diagnosis and minimal residual disease monitoring. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of hematological malignancies, including the potential to integrate modalities. © 2012 International Society for Advancement of Cytometry.</description><subject>acute leukemia</subject><subject>Acute myeloid leukemia</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Anthraquinones</subject><subject>Antibodies</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow - pathology</subject><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoplasm - genetics</subject><subject>Cytoplasm - metabolism</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Gene Expression</subject><subject>hematology</subject><subject>Humans</subject><subject>imaging flow cytometry</subject><subject>Leukemia, Myeloid, Acute - diagnosis</subject><subject>Leukemia, Myeloid, Acute - genetics</subject><subject>Leukemia, Myeloid, Acute - metabolism</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Leukocytes, Mononuclear - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Neoplasm, Residual - diagnosis</subject><subject>Neoplasm, Residual - genetics</subject><subject>Neoplasm, Residual - metabolism</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>nucleophosmin</subject><subject>Research Design</subject><subject>Sensitivity and Specificity</subject><subject>Staining and Labeling</subject><issn>1552-4922</issn><issn>1552-4930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9PgzAUxxujcXN682w4epDZPqDQo5k_45Jd5sETKeUxMUCRQib_vWXMHfXU1-TzPnnfLyGXjM4ZpXCr-lbP5RyAMX5EpiwIwPWFR48PM8CEnBnzSakXUA9OyQRA8EhwPiWv99iianNdOTpzBlddSFPmyqk6VaCuP7T9VQ5-1w0aM3BJ7-Sl3OTVxskKvd0tldg2_Tk5yWRh8GL_zsjb48N68ewuV08vi7ulq_wQuOtLGrJE8YwDFzJlCWAkeaREGCaCQ6LskUpmCDKAIEUvYn7qoZB2zgJOQ29Grkdv3eivDk0bl7lRWBSyQt2ZmNnI3Pcp5_-jlAtgNIoG9GZEVaONaTCL68bmbHoLxUPT8RA0lvGuaYtf7c1dUmJ6gH-rtYA3Atu8wP5PWbx4X69G7Q8hA4s7</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Grimwade, Lizz</creator><creator>Gudgin, Emma</creator><creator>Bloxham, David</creator><creator>Bottley, Graham</creator><creator>Vassiliou, George</creator><creator>Huntly, Brian</creator><creator>Scott, Mike A.</creator><creator>Erber, Wendy N.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201210</creationdate><title>Detection of cytoplasmic nucleophosmin expression by imaging flow cytometry</title><author>Grimwade, Lizz ; Gudgin, Emma ; Bloxham, David ; Bottley, Graham ; Vassiliou, George ; Huntly, Brian ; Scott, Mike A. ; Erber, Wendy N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4726-4a071bc6f6269ad1b2e8a68c977b962bc503cafe2a525de3814d3e9a5def56073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>acute leukemia</topic><topic>Acute myeloid leukemia</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Anthraquinones</topic><topic>Antibodies</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow - pathology</topic><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytoplasm - genetics</topic><topic>Cytoplasm - metabolism</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Gene Expression</topic><topic>hematology</topic><topic>Humans</topic><topic>imaging flow cytometry</topic><topic>Leukemia, Myeloid, Acute - diagnosis</topic><topic>Leukemia, Myeloid, Acute - genetics</topic><topic>Leukemia, Myeloid, Acute - metabolism</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Leukocytes, Mononuclear - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Neoplasm, Residual - diagnosis</topic><topic>Neoplasm, Residual - genetics</topic><topic>Neoplasm, Residual - metabolism</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>nucleophosmin</topic><topic>Research Design</topic><topic>Sensitivity and Specificity</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grimwade, Lizz</creatorcontrib><creatorcontrib>Gudgin, Emma</creatorcontrib><creatorcontrib>Bloxham, David</creatorcontrib><creatorcontrib>Bottley, Graham</creatorcontrib><creatorcontrib>Vassiliou, George</creatorcontrib><creatorcontrib>Huntly, Brian</creatorcontrib><creatorcontrib>Scott, Mike A.</creatorcontrib><creatorcontrib>Erber, Wendy N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Cytometry. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grimwade, Lizz</au><au>Gudgin, Emma</au><au>Bloxham, David</au><au>Bottley, Graham</au><au>Vassiliou, George</au><au>Huntly, Brian</au><au>Scott, Mike A.</au><au>Erber, Wendy N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of cytoplasmic nucleophosmin expression by imaging flow cytometry</atitle><jtitle>Cytometry. Part A</jtitle><addtitle>Cytometry A</addtitle><date>2012-10</date><risdate>2012</risdate><volume>81A</volume><issue>10</issue><spage>896</spage><epage>900</epage><pages>896-900</pages><issn>1552-4922</issn><eissn>1552-4930</eissn><abstract>Mutations within the nucleophosmin NPM1 gene occur in approximately one‐third of cases of acute myeloid leukemia (AML). These mutations result in cytoplasmic accumulation of the mutant NPM protein. NPM1 mutations are currently detected by molecular methods. Using samples from 37 AML patients, we investigated whether imaging flow cytometry could be a viable alternative to this current technique. Bone marrow/peripheral blood cells were stained with anti‐NPM antibody and DRAQ5 nuclear stain, and data were acquired on an ImageStream imaging flow cytometer (Amnis Corp., Seattle, USA). Using the similarity feature for data analysis, we demonstrated that this technique could successfully identify cases of AML with a NPM1 mutation based on cytoplasmic NPM protein staining (at similarity threshold of 1.1 sensitivity 88% and specificity 90%). Combining data of mean fluorescence intensity and % dissimilar staining in a 0–2 scoring system further improved the sensitivity (100%). Imaging flow cytometry has the potential to be included as part of a standard flow cytometry antibody panel to identify potential NPM1 mutations as part of diagnosis and minimal residual disease monitoring. Imaging flow cytometry is an exciting technology that has many possible applications in the diagnosis of hematological malignancies, including the potential to integrate modalities. © 2012 International Society for Advancement of Cytometry.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22968966</pmid><doi>10.1002/cyto.a.22116</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1552-4922 |
ispartof | Cytometry. Part A, 2012-10, Vol.81A (10), p.896-900 |
issn | 1552-4922 1552-4930 |
language | eng |
recordid | cdi_proquest_miscellaneous_1492644066 |
source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
subjects | acute leukemia Acute myeloid leukemia Adult Aged Aged, 80 and over Anthraquinones Antibodies Bone Marrow - metabolism Bone Marrow - pathology Cell Nucleus - genetics Cell Nucleus - metabolism Cytoplasm - genetics Cytoplasm - metabolism Female Flow Cytometry Gene Expression hematology Humans imaging flow cytometry Leukemia, Myeloid, Acute - diagnosis Leukemia, Myeloid, Acute - genetics Leukemia, Myeloid, Acute - metabolism Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - pathology Male Middle Aged Mutation Neoplasm, Residual - diagnosis Neoplasm, Residual - genetics Neoplasm, Residual - metabolism Nuclear Proteins - genetics Nuclear Proteins - metabolism nucleophosmin Research Design Sensitivity and Specificity Staining and Labeling |
title | Detection of cytoplasmic nucleophosmin expression by imaging flow cytometry |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T13%3A08%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20cytoplasmic%20nucleophosmin%20expression%20by%20imaging%20flow%20cytometry&rft.jtitle=Cytometry.%20Part%20A&rft.au=Grimwade,%20Lizz&rft.date=2012-10&rft.volume=81A&rft.issue=10&rft.spage=896&rft.epage=900&rft.pages=896-900&rft.issn=1552-4922&rft.eissn=1552-4930&rft_id=info:doi/10.1002/cyto.a.22116&rft_dat=%3Cproquest_cross%3E1069210886%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1069210886&rft_id=info:pmid/22968966&rfr_iscdi=true |