Mutants of the pentose-fermenting yeast Pachysolen tannophilus tolerant to hardwood spent sulfite liquor and acetic acid
A strain development program was initiated to improve the tolerance of the pentose-fermenting yeast Pachysolen tannophilus to inhibitors in lignocellulosic hydrolysates. Several rounds of UV mutagenesis followed by screening were used to select for mutants of P. tannophilus NRRL Y2460 with improved...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2014, Vol.105 (1), p.29-43 |
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| container_title | Antonie van Leeuwenhoek |
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| creator | Harner, Nicole K. Bajwa, Paramjit K. Habash, Marc B. Trevors, Jack T. Austin, Glen D. Lee, Hung |
| description | A strain development program was initiated to improve the tolerance of the pentose-fermenting yeast
Pachysolen tannophilus
to inhibitors in lignocellulosic hydrolysates. Several rounds of UV mutagenesis followed by screening were used to select for mutants of
P. tannophilus
NRRL Y2460 with improved tolerance to hardwood spent sulfite liquor (HW SSL) and acetic acid in separate selection lines. The wild type (WT) strain grew in 50 % (v/v) HW SSL while third round HW SSL mutants (designated UHW301, UHW302 and UHW303) grew in 60 % (v/v) HW SSL, with two of these isolates (UHW302 and UHW303) being viable and growing, respectively, in 70 % (v/v) HW SSL. In defined liquid media containing acetic acid, the WT strain grew in 0.70 % (w/v) acetic acid, while third round acetic acid mutants (designated UAA301, UAA302 and UAA303) grew in 0.80 % (w/v) acetic acid, with one isolate (UAA302) growing in 0.90 % (w/v) acetic acid. Cross-tolerance of HW SSL-tolerant mutants to acetic acid and vice versa was observed with UHW303 able to grow in 0.90 % (w/v) acetic acid and UAA302 growing in 60 % (v/v) HW SSL. The UV-induced mutants retained the ability to ferment glucose and xylose to ethanol in defined media. These mutants of
P. tannophilus
are of considerable interest for bioconversion of the sugars in lignocellulosic hydrolysates to ethanol. |
| doi_str_mv | 10.1007/s10482-013-0050-y |
| format | Article |
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Pachysolen tannophilus
to inhibitors in lignocellulosic hydrolysates. Several rounds of UV mutagenesis followed by screening were used to select for mutants of
P. tannophilus
NRRL Y2460 with improved tolerance to hardwood spent sulfite liquor (HW SSL) and acetic acid in separate selection lines. The wild type (WT) strain grew in 50 % (v/v) HW SSL while third round HW SSL mutants (designated UHW301, UHW302 and UHW303) grew in 60 % (v/v) HW SSL, with two of these isolates (UHW302 and UHW303) being viable and growing, respectively, in 70 % (v/v) HW SSL. In defined liquid media containing acetic acid, the WT strain grew in 0.70 % (w/v) acetic acid, while third round acetic acid mutants (designated UAA301, UAA302 and UAA303) grew in 0.80 % (w/v) acetic acid, with one isolate (UAA302) growing in 0.90 % (w/v) acetic acid. Cross-tolerance of HW SSL-tolerant mutants to acetic acid and vice versa was observed with UHW303 able to grow in 0.90 % (w/v) acetic acid and UAA302 growing in 60 % (v/v) HW SSL. The UV-induced mutants retained the ability to ferment glucose and xylose to ethanol in defined media. These mutants of
P. tannophilus
are of considerable interest for bioconversion of the sugars in lignocellulosic hydrolysates to ethanol.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-013-0050-y</identifier><identifier>PMID: 24122119</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acetic acid ; Acetic Acid - metabolism ; Biomedical and Life Sciences ; Bioreactors ; Biosynthesis ; Biotechnology ; Ethanol ; Ethanol - metabolism ; Fermentation ; Glucose - metabolism ; Life Sciences ; Lignocellulose ; Medical Microbiology ; Microbiology ; Mutagenesis ; Mutants ; Original Paper ; Pachysolen tannophilus ; Pentoses - metabolism ; Plant Sciences ; Saccharomycetales - genetics ; Saccharomycetales - metabolism ; Soil Science & Conservation ; Studies ; Sulfite liquors ; Sulfites - metabolism ; Wood - microbiology ; Xylose - metabolism ; Yeasts</subject><ispartof>Antonie van Leeuwenhoek, 2014, Vol.105 (1), p.29-43</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-4fcc5405e8b993d7c4b509ff03addb1f5b10b5efad3aa63ebe5dd1f2893c25893</citedby><cites>FETCH-LOGICAL-c405t-4fcc5405e8b993d7c4b509ff03addb1f5b10b5efad3aa63ebe5dd1f2893c25893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-013-0050-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-013-0050-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24122119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harner, Nicole K.</creatorcontrib><creatorcontrib>Bajwa, Paramjit K.</creatorcontrib><creatorcontrib>Habash, Marc B.</creatorcontrib><creatorcontrib>Trevors, Jack T.</creatorcontrib><creatorcontrib>Austin, Glen D.</creatorcontrib><creatorcontrib>Lee, Hung</creatorcontrib><title>Mutants of the pentose-fermenting yeast Pachysolen tannophilus tolerant to hardwood spent sulfite liquor and acetic acid</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>A strain development program was initiated to improve the tolerance of the pentose-fermenting yeast
Pachysolen tannophilus
to inhibitors in lignocellulosic hydrolysates. Several rounds of UV mutagenesis followed by screening were used to select for mutants of
P. tannophilus
NRRL Y2460 with improved tolerance to hardwood spent sulfite liquor (HW SSL) and acetic acid in separate selection lines. The wild type (WT) strain grew in 50 % (v/v) HW SSL while third round HW SSL mutants (designated UHW301, UHW302 and UHW303) grew in 60 % (v/v) HW SSL, with two of these isolates (UHW302 and UHW303) being viable and growing, respectively, in 70 % (v/v) HW SSL. In defined liquid media containing acetic acid, the WT strain grew in 0.70 % (w/v) acetic acid, while third round acetic acid mutants (designated UAA301, UAA302 and UAA303) grew in 0.80 % (w/v) acetic acid, with one isolate (UAA302) growing in 0.90 % (w/v) acetic acid. Cross-tolerance of HW SSL-tolerant mutants to acetic acid and vice versa was observed with UHW303 able to grow in 0.90 % (w/v) acetic acid and UAA302 growing in 60 % (v/v) HW SSL. The UV-induced mutants retained the ability to ferment glucose and xylose to ethanol in defined media. These mutants of
P. tannophilus
are of considerable interest for bioconversion of the sugars in lignocellulosic hydrolysates to ethanol.</description><subject>Acetic acid</subject><subject>Acetic Acid - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>Fermentation</subject><subject>Glucose - metabolism</subject><subject>Life Sciences</subject><subject>Lignocellulose</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Original Paper</subject><subject>Pachysolen tannophilus</subject><subject>Pentoses - metabolism</subject><subject>Plant Sciences</subject><subject>Saccharomycetales - genetics</subject><subject>Saccharomycetales - metabolism</subject><subject>Soil Science & Conservation</subject><subject>Studies</subject><subject>Sulfite liquors</subject><subject>Sulfites - 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metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Ethanol</topic><topic>Ethanol - metabolism</topic><topic>Fermentation</topic><topic>Glucose - metabolism</topic><topic>Life Sciences</topic><topic>Lignocellulose</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Original Paper</topic><topic>Pachysolen tannophilus</topic><topic>Pentoses - metabolism</topic><topic>Plant Sciences</topic><topic>Saccharomycetales - genetics</topic><topic>Saccharomycetales - metabolism</topic><topic>Soil Science & Conservation</topic><topic>Studies</topic><topic>Sulfite liquors</topic><topic>Sulfites - metabolism</topic><topic>Wood - microbiology</topic><topic>Xylose - metabolism</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harner, Nicole K.</creatorcontrib><creatorcontrib>Bajwa, Paramjit K.</creatorcontrib><creatorcontrib>Habash, Marc B.</creatorcontrib><creatorcontrib>Trevors, Jack T.</creatorcontrib><creatorcontrib>Austin, Glen D.</creatorcontrib><creatorcontrib>Lee, Hung</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harner, Nicole K.</au><au>Bajwa, Paramjit K.</au><au>Habash, Marc B.</au><au>Trevors, Jack T.</au><au>Austin, Glen D.</au><au>Lee, Hung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutants of the pentose-fermenting yeast Pachysolen tannophilus tolerant to hardwood spent sulfite liquor and acetic acid</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2014</date><risdate>2014</risdate><volume>105</volume><issue>1</issue><spage>29</spage><epage>43</epage><pages>29-43</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>A strain development program was initiated to improve the tolerance of the pentose-fermenting yeast
Pachysolen tannophilus
to inhibitors in lignocellulosic hydrolysates. Several rounds of UV mutagenesis followed by screening were used to select for mutants of
P. tannophilus
NRRL Y2460 with improved tolerance to hardwood spent sulfite liquor (HW SSL) and acetic acid in separate selection lines. The wild type (WT) strain grew in 50 % (v/v) HW SSL while third round HW SSL mutants (designated UHW301, UHW302 and UHW303) grew in 60 % (v/v) HW SSL, with two of these isolates (UHW302 and UHW303) being viable and growing, respectively, in 70 % (v/v) HW SSL. In defined liquid media containing acetic acid, the WT strain grew in 0.70 % (w/v) acetic acid, while third round acetic acid mutants (designated UAA301, UAA302 and UAA303) grew in 0.80 % (w/v) acetic acid, with one isolate (UAA302) growing in 0.90 % (w/v) acetic acid. Cross-tolerance of HW SSL-tolerant mutants to acetic acid and vice versa was observed with UHW303 able to grow in 0.90 % (w/v) acetic acid and UAA302 growing in 60 % (v/v) HW SSL. The UV-induced mutants retained the ability to ferment glucose and xylose to ethanol in defined media. These mutants of
P. tannophilus
are of considerable interest for bioconversion of the sugars in lignocellulosic hydrolysates to ethanol.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24122119</pmid><doi>10.1007/s10482-013-0050-y</doi><tpages>15</tpages></addata></record> |
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| ispartof | Antonie van Leeuwenhoek, 2014, Vol.105 (1), p.29-43 |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Acetic acid Acetic Acid - metabolism Biomedical and Life Sciences Bioreactors Biosynthesis Biotechnology Ethanol Ethanol - metabolism Fermentation Glucose - metabolism Life Sciences Lignocellulose Medical Microbiology Microbiology Mutagenesis Mutants Original Paper Pachysolen tannophilus Pentoses - metabolism Plant Sciences Saccharomycetales - genetics Saccharomycetales - metabolism Soil Science & Conservation Studies Sulfite liquors Sulfites - metabolism Wood - microbiology Xylose - metabolism Yeasts |
| title | Mutants of the pentose-fermenting yeast Pachysolen tannophilus tolerant to hardwood spent sulfite liquor and acetic acid |
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