FLUORESCENT PROBES FOR THE PEPTIDES INVESTIGATION

This paper aimed to investigate the use of new probes E-176, Κ8-3000 and Κ-1300 to research the specificity of peptides in the extracts of cryo-preserved fragments of organs of pigs' and piglets' and their interaction with blood serum albumin. Extracts of cryopreserved fragments of skin of new-born piglets', spleen of pigs', heart of mature pigs' and new-born piglets' cleared of proteins were used in the research. Titration of extracts with investigated probes revealed differences in the peptides properties that are a part of them, to bind probes, reflecting tissue specificity of peptide drugs. Adding the extracts to albumin solution triggered quenching of intrinsic fluorescence of albumin and affected the state of tryptophan residues of the protein. Investigated probes show a high sensitivity to the interaction with blood serum albumin substances of peptide nature and could be recommended as new fluorescent probes for use in the biomedical researches.