Gold nanoparticle-assisted primer walking for closing the human chromosomal gap

The finished sequence of the human genome still contains 260 euchromatic gaps. All the PCR-based genome walking techniques used to close gaps have common limitations, such as low efficiency and low specificity. We herein describe a strategy to solve this problem by employing gold nanoparticles (AuNP...

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Veröffentlicht in:	Analytical methods 2013-01, Vol.5 (18), p.4746-4749
Hauptverfasser:	Li, Haikuo, Shi, Baochen, Li, Xueling, Nyegaard, Steffen, LÜ, Ming, LÜ, Junhong, Cao, Xueyan, Huang, Qing, Fan, Chunhai, Chen, Runsheng, Hu, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Conserved sequence
Online-Zugang:	Volltext
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