Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue
•ERα/ERβ ratios are defined in T47D-ERβ cells with tetracycline dependent ERβ.•ERα/ERβ ratios of T47D-ERβ cells can mimic ratios in breast, prostate and uterus.•Conditions at which the T47D-ERb cells best mimic in vivo tissues are established.•Estradiol induced cell proliferation varies with the ERα...
Gespeichert in:
| Veröffentlicht in: | Toxicology in vitro 2013-09, Vol.27 (6), p.1753-1761 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1761 |
|---|---|
| container_issue | 6 |
| container_start_page | 1753 |
| container_title | Toxicology in vitro |
| container_volume | 27 |
| creator | Evers, N.M. van de Klundert, T.M.C. van Aesch, Y.M. Wang, S. de Roos, W.K. Romano, A. de Haan, L.H.J. Murk, A.J. Ederveen, A.G.H. Rietjens, I.M.C.M. Groten, J.P. |
| description | •ERα/ERβ ratios are defined in T47D-ERβ cells with tetracycline dependent ERβ.•ERα/ERβ ratios of T47D-ERβ cells can mimic ratios in breast, prostate and uterus.•Conditions at which the T47D-ERb cells best mimic in vivo tissues are established.•Estradiol induced cell proliferation varies with the ERα/ERβ ratio.•ERβ levels in MCF-7 and native T47D cell lines do not reflect levels in tissues.
T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression and constant ERα expression can be used to investigate effects of varying ERα/ERβ ratios on estrogen-induced cellular responses. This study defines conditions at which ERα/ERβ ratios in T47D-ERβ cells best mimic ERα/ERβ ratios in breast and other estrogen-sensitive tissues in vivo in rat as well as in human.
Protein and mRNA levels of ERα and ERβ were analyzed in T47D-ERβ cells exposed to a range of tetracycline concentrations and compared to ERα and ERβ levels found in breast, prostate, and uterus from rat and human origin.
The ERα/ERβ ratio in T47D-ERβ cells exposed to >150ng/ml tetracycline is comparable to the ratio found in rat mammary gland and in human breast tissue. The ERα/ERβ ratio of other estrogen-sensitive rat and human tissues can also be mimicked in T47D-ERβ cells. The ERα/ERβ ratio found in MCF-7 and native T47D breast cancer cell lines did not reflect ratios in analyzed rat and human tissues, which further supports the use of T47D-ERβ cells as model for estrogen-responsive tissues. Using 17β-estradiol and the T47D-ERβ cells under the conditions defined to mimic various tissues it could be demonstrated how these different tissues vary in their proliferative response. |
| doi_str_mv | 10.1016/j.tiv.2013.04.014 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1492618764</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0887233313001215</els_id><sourcerecordid>1492618764</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-71f080542e433649d01886a61bcc504cb51c6433389361de7a5cb9b2e5e980d73</originalsourceid><addsrcrecordid>eNp9kE1uFDEQhS0EIsPAAdggL9l0p9x2u91ihfJDkCJFQmFtue0axaMe92C7AzlBzgMHyZnw_MAyK5dc773S-wh5z6BmwOTpus7-vm6A8RpEDUy8IAumur7irOtekgUo1VUN5_yEvElpDQCtauA1OWm4VMB5syCPV_PGBHoruvPq4tvTHzpENClTa4LFSC2OY6I_fb6jGXM09sGOPmDlcIvBYch0b8Jf24gp-SnQiKsR7f7_9-l-GU32U6I-7CZqgqN3-5vHS9mnNONb8mplxoTvju-SfL-8uD27qq5vvnw9-3xdWa5krjq2AgWtaFBwLkXvgCkljWSDtS0IO7TMyrLiqueSOexMa4d-aLDFXoHr-JJ8PORu4_RjxpT1xqddSxNwmpNmom9kYVhCloQdpDZOKZVeehv9xsQHzUDv-Ou1Lvz1jr8GoQv_4vlwjJ-HDbr_jn_Ai-DTQYCl5L3HqJP1WFg7Hws27Sb_TPxfU5OYtg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1492618764</pqid></control><display><type>article</type><title>Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Evers, N.M. ; van de Klundert, T.M.C. ; van Aesch, Y.M. ; Wang, S. ; de Roos, W.K. ; Romano, A. ; de Haan, L.H.J. ; Murk, A.J. ; Ederveen, A.G.H. ; Rietjens, I.M.C.M. ; Groten, J.P.</creator><creatorcontrib>Evers, N.M. ; van de Klundert, T.M.C. ; van Aesch, Y.M. ; Wang, S. ; de Roos, W.K. ; Romano, A. ; de Haan, L.H.J. ; Murk, A.J. ; Ederveen, A.G.H. ; Rietjens, I.M.C.M. ; Groten, J.P.</creatorcontrib><description>•ERα/ERβ ratios are defined in T47D-ERβ cells with tetracycline dependent ERβ.•ERα/ERβ ratios of T47D-ERβ cells can mimic ratios in breast, prostate and uterus.•Conditions at which the T47D-ERb cells best mimic in vivo tissues are established.•Estradiol induced cell proliferation varies with the ERα/ERβ ratio.•ERβ levels in MCF-7 and native T47D cell lines do not reflect levels in tissues.
T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression and constant ERα expression can be used to investigate effects of varying ERα/ERβ ratios on estrogen-induced cellular responses. This study defines conditions at which ERα/ERβ ratios in T47D-ERβ cells best mimic ERα/ERβ ratios in breast and other estrogen-sensitive tissues in vivo in rat as well as in human.
Protein and mRNA levels of ERα and ERβ were analyzed in T47D-ERβ cells exposed to a range of tetracycline concentrations and compared to ERα and ERβ levels found in breast, prostate, and uterus from rat and human origin.
The ERα/ERβ ratio in T47D-ERβ cells exposed to >150ng/ml tetracycline is comparable to the ratio found in rat mammary gland and in human breast tissue. The ERα/ERβ ratio of other estrogen-sensitive rat and human tissues can also be mimicked in T47D-ERβ cells. The ERα/ERβ ratio found in MCF-7 and native T47D breast cancer cell lines did not reflect ratios in analyzed rat and human tissues, which further supports the use of T47D-ERβ cells as model for estrogen-responsive tissues. Using 17β-estradiol and the T47D-ERβ cells under the conditions defined to mimic various tissues it could be demonstrated how these different tissues vary in their proliferative response.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2013.04.014</identifier><identifier>PMID: 23680332</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adult ; Aged ; Animals ; Breast - metabolism ; Breast Neoplasms - metabolism ; Breast tissue ; Cell Line, Tumor ; ERα/ERβ ratio ; Estrogen Receptor alpha - metabolism ; Estrogen Receptor beta - metabolism ; Estrogen receptors ; Estrogen-sensitive tissue ; Female ; Humans ; In vitro–in vivo comparison ; Male ; Mammary Glands, Animal - metabolism ; Middle Aged ; Prostate - metabolism ; Rats ; T47D-ERβ cells with tetracycline dependent ERβ expression ; Tetracycline ; Uterus - metabolism</subject><ispartof>Toxicology in vitro, 2013-09, Vol.27 (6), p.1753-1761</ispartof><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-71f080542e433649d01886a61bcc504cb51c6433389361de7a5cb9b2e5e980d73</citedby><cites>FETCH-LOGICAL-c386t-71f080542e433649d01886a61bcc504cb51c6433389361de7a5cb9b2e5e980d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0887233313001215$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23680332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Evers, N.M.</creatorcontrib><creatorcontrib>van de Klundert, T.M.C.</creatorcontrib><creatorcontrib>van Aesch, Y.M.</creatorcontrib><creatorcontrib>Wang, S.</creatorcontrib><creatorcontrib>de Roos, W.K.</creatorcontrib><creatorcontrib>Romano, A.</creatorcontrib><creatorcontrib>de Haan, L.H.J.</creatorcontrib><creatorcontrib>Murk, A.J.</creatorcontrib><creatorcontrib>Ederveen, A.G.H.</creatorcontrib><creatorcontrib>Rietjens, I.M.C.M.</creatorcontrib><creatorcontrib>Groten, J.P.</creatorcontrib><title>Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>•ERα/ERβ ratios are defined in T47D-ERβ cells with tetracycline dependent ERβ.•ERα/ERβ ratios of T47D-ERβ cells can mimic ratios in breast, prostate and uterus.•Conditions at which the T47D-ERb cells best mimic in vivo tissues are established.•Estradiol induced cell proliferation varies with the ERα/ERβ ratio.•ERβ levels in MCF-7 and native T47D cell lines do not reflect levels in tissues.
T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression and constant ERα expression can be used to investigate effects of varying ERα/ERβ ratios on estrogen-induced cellular responses. This study defines conditions at which ERα/ERβ ratios in T47D-ERβ cells best mimic ERα/ERβ ratios in breast and other estrogen-sensitive tissues in vivo in rat as well as in human.
Protein and mRNA levels of ERα and ERβ were analyzed in T47D-ERβ cells exposed to a range of tetracycline concentrations and compared to ERα and ERβ levels found in breast, prostate, and uterus from rat and human origin.
The ERα/ERβ ratio in T47D-ERβ cells exposed to >150ng/ml tetracycline is comparable to the ratio found in rat mammary gland and in human breast tissue. The ERα/ERβ ratio of other estrogen-sensitive rat and human tissues can also be mimicked in T47D-ERβ cells. The ERα/ERβ ratio found in MCF-7 and native T47D breast cancer cell lines did not reflect ratios in analyzed rat and human tissues, which further supports the use of T47D-ERβ cells as model for estrogen-responsive tissues. Using 17β-estradiol and the T47D-ERβ cells under the conditions defined to mimic various tissues it could be demonstrated how these different tissues vary in their proliferative response.</description><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Breast - metabolism</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast tissue</subject><subject>Cell Line, Tumor</subject><subject>ERα/ERβ ratio</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Estrogen receptors</subject><subject>Estrogen-sensitive tissue</subject><subject>Female</subject><subject>Humans</subject><subject>In vitro–in vivo comparison</subject><subject>Male</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Middle Aged</subject><subject>Prostate - metabolism</subject><subject>Rats</subject><subject>T47D-ERβ cells with tetracycline dependent ERβ expression</subject><subject>Tetracycline</subject><subject>Uterus - metabolism</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1uFDEQhS0EIsPAAdggL9l0p9x2u91ihfJDkCJFQmFtue0axaMe92C7AzlBzgMHyZnw_MAyK5dc773S-wh5z6BmwOTpus7-vm6A8RpEDUy8IAumur7irOtekgUo1VUN5_yEvElpDQCtauA1OWm4VMB5syCPV_PGBHoruvPq4tvTHzpENClTa4LFSC2OY6I_fb6jGXM09sGOPmDlcIvBYch0b8Jf24gp-SnQiKsR7f7_9-l-GU32U6I-7CZqgqN3-5vHS9mnNONb8mplxoTvju-SfL-8uD27qq5vvnw9-3xdWa5krjq2AgWtaFBwLkXvgCkljWSDtS0IO7TMyrLiqueSOexMa4d-aLDFXoHr-JJ8PORu4_RjxpT1xqddSxNwmpNmom9kYVhCloQdpDZOKZVeehv9xsQHzUDv-Ou1Lvz1jr8GoQv_4vlwjJ-HDbr_jn_Ai-DTQYCl5L3HqJP1WFg7Hws27Sb_TPxfU5OYtg</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Evers, N.M.</creator><creator>van de Klundert, T.M.C.</creator><creator>van Aesch, Y.M.</creator><creator>Wang, S.</creator><creator>de Roos, W.K.</creator><creator>Romano, A.</creator><creator>de Haan, L.H.J.</creator><creator>Murk, A.J.</creator><creator>Ederveen, A.G.H.</creator><creator>Rietjens, I.M.C.M.</creator><creator>Groten, J.P.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20130901</creationdate><title>Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue</title><author>Evers, N.M. ; van de Klundert, T.M.C. ; van Aesch, Y.M. ; Wang, S. ; de Roos, W.K. ; Romano, A. ; de Haan, L.H.J. ; Murk, A.J. ; Ederveen, A.G.H. ; Rietjens, I.M.C.M. ; Groten, J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-71f080542e433649d01886a61bcc504cb51c6433389361de7a5cb9b2e5e980d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Breast - metabolism</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast tissue</topic><topic>Cell Line, Tumor</topic><topic>ERα/ERβ ratio</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Estrogen receptors</topic><topic>Estrogen-sensitive tissue</topic><topic>Female</topic><topic>Humans</topic><topic>In vitro–in vivo comparison</topic><topic>Male</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Middle Aged</topic><topic>Prostate - metabolism</topic><topic>Rats</topic><topic>T47D-ERβ cells with tetracycline dependent ERβ expression</topic><topic>Tetracycline</topic><topic>Uterus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Evers, N.M.</creatorcontrib><creatorcontrib>van de Klundert, T.M.C.</creatorcontrib><creatorcontrib>van Aesch, Y.M.</creatorcontrib><creatorcontrib>Wang, S.</creatorcontrib><creatorcontrib>de Roos, W.K.</creatorcontrib><creatorcontrib>Romano, A.</creatorcontrib><creatorcontrib>de Haan, L.H.J.</creatorcontrib><creatorcontrib>Murk, A.J.</creatorcontrib><creatorcontrib>Ederveen, A.G.H.</creatorcontrib><creatorcontrib>Rietjens, I.M.C.M.</creatorcontrib><creatorcontrib>Groten, J.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Evers, N.M.</au><au>van de Klundert, T.M.C.</au><au>van Aesch, Y.M.</au><au>Wang, S.</au><au>de Roos, W.K.</au><au>Romano, A.</au><au>de Haan, L.H.J.</au><au>Murk, A.J.</au><au>Ederveen, A.G.H.</au><au>Rietjens, I.M.C.M.</au><au>Groten, J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>27</volume><issue>6</issue><spage>1753</spage><epage>1761</epage><pages>1753-1761</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>•ERα/ERβ ratios are defined in T47D-ERβ cells with tetracycline dependent ERβ.•ERα/ERβ ratios of T47D-ERβ cells can mimic ratios in breast, prostate and uterus.•Conditions at which the T47D-ERb cells best mimic in vivo tissues are established.•Estradiol induced cell proliferation varies with the ERα/ERβ ratio.•ERβ levels in MCF-7 and native T47D cell lines do not reflect levels in tissues.
T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression and constant ERα expression can be used to investigate effects of varying ERα/ERβ ratios on estrogen-induced cellular responses. This study defines conditions at which ERα/ERβ ratios in T47D-ERβ cells best mimic ERα/ERβ ratios in breast and other estrogen-sensitive tissues in vivo in rat as well as in human.
Protein and mRNA levels of ERα and ERβ were analyzed in T47D-ERβ cells exposed to a range of tetracycline concentrations and compared to ERα and ERβ levels found in breast, prostate, and uterus from rat and human origin.
The ERα/ERβ ratio in T47D-ERβ cells exposed to >150ng/ml tetracycline is comparable to the ratio found in rat mammary gland and in human breast tissue. The ERα/ERβ ratio of other estrogen-sensitive rat and human tissues can also be mimicked in T47D-ERβ cells. The ERα/ERβ ratio found in MCF-7 and native T47D breast cancer cell lines did not reflect ratios in analyzed rat and human tissues, which further supports the use of T47D-ERβ cells as model for estrogen-responsive tissues. Using 17β-estradiol and the T47D-ERβ cells under the conditions defined to mimic various tissues it could be demonstrated how these different tissues vary in their proliferative response.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23680332</pmid><doi>10.1016/j.tiv.2013.04.014</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-2333 |
| ispartof | Toxicology in vitro, 2013-09, Vol.27 (6), p.1753-1761 |
| issn | 0887-2333 1879-3177 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1492618764 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Aged Animals Breast - metabolism Breast Neoplasms - metabolism Breast tissue Cell Line, Tumor ERα/ERβ ratio Estrogen Receptor alpha - metabolism Estrogen Receptor beta - metabolism Estrogen receptors Estrogen-sensitive tissue Female Humans In vitro–in vivo comparison Male Mammary Glands, Animal - metabolism Middle Aged Prostate - metabolism Rats T47D-ERβ cells with tetracycline dependent ERβ expression Tetracycline Uterus - metabolism |
| title | Human T47D-ERβ breast cancer cells with tetracycline-dependent ERβ expression reflect ERα/ERβ ratios in rat and human breast tissue |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T17%3A58%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20T47D-ER%CE%B2%20breast%20cancer%20cells%20with%20tetracycline-dependent%20ER%CE%B2%20expression%20reflect%20ER%CE%B1/ER%CE%B2%20ratios%20in%20rat%20and%20human%20breast%20tissue&rft.jtitle=Toxicology%20in%20vitro&rft.au=Evers,%20N.M.&rft.date=2013-09-01&rft.volume=27&rft.issue=6&rft.spage=1753&rft.epage=1761&rft.pages=1753-1761&rft.issn=0887-2333&rft.eissn=1879-3177&rft_id=info:doi/10.1016/j.tiv.2013.04.014&rft_dat=%3Cproquest_cross%3E1492618764%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1492618764&rft_id=info:pmid/23680332&rft_els_id=S0887233313001215&rfr_iscdi=true |