Comparison of stool microbiota compositions, stool alpha1-antitrypsin and calprotectin concentrations, and diarrhoeal morbidity of Indonesian infants fed breast milk or probiotic/prebiotic-supplemented formula
Aim The composition of faecal microbiota of babies is known to be influenced by diet. Faecal calprotectin and α1‐antitrypsin concentrations may be associated with mucosal permeability and inflammation. We aimed to assess whether there was any difference after consumption of a probiotic/prebiotic for...
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| Veröffentlicht in: | Journal of paediatrics and child health 2013-12, Vol.49 (12), p.1032-1039 |
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| container_title | Journal of paediatrics and child health |
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| creator | Oswari, Hanifah Prayitno, Lamtorogung Dwipoerwantoro, Pramita G Firmansyah, Agus Makrides, Maria Lawley, Blair Kuhn-Sherlock, Barbara Cleghorn, Geoffrey Tannock, Gerald W |
| description | Aim
The composition of faecal microbiota of babies is known to be influenced by diet. Faecal calprotectin and α1‐antitrypsin concentrations may be associated with mucosal permeability and inflammation. We aimed to assess whether there was any difference after consumption of a probiotic/prebiotic formula on faecal microbiota composition, calprotectin and α1‐antitrypsin levels, and diarrhoea in comparison with breast milk‐fed Indonesian infants.
Methods
One hundred sixty infants, 2 to 6 weeks old, were recruited to the study. They were either breastfed or formula fed (80 per group). Faecal samples were collected at recruitment and 3 months later. Bacterial groups characteristic of the human faecal microbiota were quantified in faeces by quantitative polymerase chain reaction. Calprotectin and α1‐antitrypsin concentrations were measured using commercial kits. Details of diarrhoeal morbidity were documented and rated for severity.
Results
The compositions of the faecal microbiota of formula‐fed compared with breast milk‐fed children were similar except that the probiotic strain Bifidobacterium animalis subsp. lactis DR10 was more abundant after 3 months consumption of the formula. Alpha1‐antitrypsin levels were higher in breastfed compared with formula‐fed infants. The occurrence of diarrhoea did not differ between the groups of babies.
Conclusion
Feeding Indonesian babies with a probiotic/prebiotic formula did not produce marked differences in the composition of the faecal microbiota in comparison with breast milk. Detrimental effects of formula feeding on biomarkers of mucosal health were not observed. |
| doi_str_mv | 10.1111/jpc.12307 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1492612694</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1467635611</sourcerecordid><originalsourceid>FETCH-LOGICAL-i4167-fbe5d057a3f968b08cfcd2a393f6de7945a4d4214371e68a76d7c16c27eae0d93</originalsourceid><addsrcrecordid>eNqNkktv1DAQxyMEog848AWQJS4cmq5fsZMjWtFSVAFSQUhcrIntqN4mdmo7gv2YfCO83aUHTvji0czvPw_NVNUrgs9JeavNrM8JZVg-qY4J57gmsuFPi40Zr3lL8FF1ktIGY0ybpn1eHVEmW0oFO65-r8M0Q3QpeBQGlHIII5qcjqF3IQPSJRySyy74dHYIwzjfAqnBZ5fjdk7OI_AG6eKPIVudi0MHr63PEQ7KHWAcxHgbLJQKIfbOuLzdFb3yJnibHHjk_FDSJjRYg_poIeXSzHiHQkTzviWnV3O0e6tOyzyPdiqFCj-EOC0jvKieDTAm-_Lwn1bfLt5_XX-orz9fXq3fXdeOEyHrobeNwY0ENnSi7XGrB20osI4NwljZ8Qa44ZRwJokVLUhhpCZCU2nBYtOx0-rtPm9p7H6xKavJJW3HEbwNS1KEd1QQKjr-H6iQgjWCkIK--QfdhCX6MsiOEozwsrtCvT5QSz9Zo-boJohb9XevBVjtgZ9utNvHOMFqdzCqHIx6OBj18cv6wSiKeq9wKdtfjwqId0pIJhv1_dOluri56VraSvWD_QG6wsd4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1466314237</pqid></control><display><type>article</type><title>Comparison of stool microbiota compositions, stool alpha1-antitrypsin and calprotectin concentrations, and diarrhoeal morbidity of Indonesian infants fed breast milk or probiotic/prebiotic-supplemented formula</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Oswari, Hanifah ; Prayitno, Lamtorogung ; Dwipoerwantoro, Pramita G ; Firmansyah, Agus ; Makrides, Maria ; Lawley, Blair ; Kuhn-Sherlock, Barbara ; Cleghorn, Geoffrey ; Tannock, Gerald W</creator><creatorcontrib>Oswari, Hanifah ; Prayitno, Lamtorogung ; Dwipoerwantoro, Pramita G ; Firmansyah, Agus ; Makrides, Maria ; Lawley, Blair ; Kuhn-Sherlock, Barbara ; Cleghorn, Geoffrey ; Tannock, Gerald W</creatorcontrib><description>Aim
The composition of faecal microbiota of babies is known to be influenced by diet. Faecal calprotectin and α1‐antitrypsin concentrations may be associated with mucosal permeability and inflammation. We aimed to assess whether there was any difference after consumption of a probiotic/prebiotic formula on faecal microbiota composition, calprotectin and α1‐antitrypsin levels, and diarrhoea in comparison with breast milk‐fed Indonesian infants.
Methods
One hundred sixty infants, 2 to 6 weeks old, were recruited to the study. They were either breastfed or formula fed (80 per group). Faecal samples were collected at recruitment and 3 months later. Bacterial groups characteristic of the human faecal microbiota were quantified in faeces by quantitative polymerase chain reaction. Calprotectin and α1‐antitrypsin concentrations were measured using commercial kits. Details of diarrhoeal morbidity were documented and rated for severity.
Results
The compositions of the faecal microbiota of formula‐fed compared with breast milk‐fed children were similar except that the probiotic strain Bifidobacterium animalis subsp. lactis DR10 was more abundant after 3 months consumption of the formula. Alpha1‐antitrypsin levels were higher in breastfed compared with formula‐fed infants. The occurrence of diarrhoea did not differ between the groups of babies.
Conclusion
Feeding Indonesian babies with a probiotic/prebiotic formula did not produce marked differences in the composition of the faecal microbiota in comparison with breast milk. Detrimental effects of formula feeding on biomarkers of mucosal health were not observed.</description><identifier>ISSN: 1034-4810</identifier><identifier>EISSN: 1440-1754</identifier><identifier>DOI: 10.1111/jpc.12307</identifier><identifier>PMID: 23782263</identifier><language>eng</language><publisher>Australia: Blackwell Publishing Ltd</publisher><subject>alpha 1-Antitrypsin - analysis ; Babies ; Baby foods ; Bifidobacterium ; Bifidobacterium - isolation & purification ; Bifidobacterium animalis subsp. lactis ; Biomarkers - analysis ; Breast Feeding ; Breastfeeding & lactation ; calprotectin ; Cohort Studies ; Diarrhea ; Feces ; Feces - chemistry ; Feces - microbiology ; Female ; Humans ; Indonesia ; Infant ; Infant Formula - chemistry ; Infant, Newborn ; Intestinal Mucosa - chemistry ; Intestinal Mucosa - microbiology ; Leukocyte L1 Antigen Complex - analysis ; Male ; Microbiology ; Microbiota ; Milk, Human ; Polymerase Chain Reaction ; prebiotic ; Prebiotics ; probiotic ; Probiotics ; α1-antitrypsin</subject><ispartof>Journal of paediatrics and child health, 2013-12, Vol.49 (12), p.1032-1039</ispartof><rights>2013 The Authors. Journal of Paediatrics and Child Health © 2013 Paediatrics and Child Health Division (Royal Australasian College of Physicians)</rights><rights>2013 The Authors. Journal of Paediatrics and Child Health © 2013 Paediatrics and Child Health Division (Royal Australasian College of Physicians).</rights><rights>Journal of Paediatrics and Child Health © 2013 Paediatrics and Child Health Division (Royal Australasian College of Physicians)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjpc.12307$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjpc.12307$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23782263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oswari, Hanifah</creatorcontrib><creatorcontrib>Prayitno, Lamtorogung</creatorcontrib><creatorcontrib>Dwipoerwantoro, Pramita G</creatorcontrib><creatorcontrib>Firmansyah, Agus</creatorcontrib><creatorcontrib>Makrides, Maria</creatorcontrib><creatorcontrib>Lawley, Blair</creatorcontrib><creatorcontrib>Kuhn-Sherlock, Barbara</creatorcontrib><creatorcontrib>Cleghorn, Geoffrey</creatorcontrib><creatorcontrib>Tannock, Gerald W</creatorcontrib><title>Comparison of stool microbiota compositions, stool alpha1-antitrypsin and calprotectin concentrations, and diarrhoeal morbidity of Indonesian infants fed breast milk or probiotic/prebiotic-supplemented formula</title><title>Journal of paediatrics and child health</title><addtitle>J Paediatr Child Health</addtitle><description>Aim
The composition of faecal microbiota of babies is known to be influenced by diet. Faecal calprotectin and α1‐antitrypsin concentrations may be associated with mucosal permeability and inflammation. We aimed to assess whether there was any difference after consumption of a probiotic/prebiotic formula on faecal microbiota composition, calprotectin and α1‐antitrypsin levels, and diarrhoea in comparison with breast milk‐fed Indonesian infants.
Methods
One hundred sixty infants, 2 to 6 weeks old, were recruited to the study. They were either breastfed or formula fed (80 per group). Faecal samples were collected at recruitment and 3 months later. Bacterial groups characteristic of the human faecal microbiota were quantified in faeces by quantitative polymerase chain reaction. Calprotectin and α1‐antitrypsin concentrations were measured using commercial kits. Details of diarrhoeal morbidity were documented and rated for severity.
Results
The compositions of the faecal microbiota of formula‐fed compared with breast milk‐fed children were similar except that the probiotic strain Bifidobacterium animalis subsp. lactis DR10 was more abundant after 3 months consumption of the formula. Alpha1‐antitrypsin levels were higher in breastfed compared with formula‐fed infants. The occurrence of diarrhoea did not differ between the groups of babies.
Conclusion
Feeding Indonesian babies with a probiotic/prebiotic formula did not produce marked differences in the composition of the faecal microbiota in comparison with breast milk. Detrimental effects of formula feeding on biomarkers of mucosal health were not observed.</description><subject>alpha 1-Antitrypsin - analysis</subject><subject>Babies</subject><subject>Baby foods</subject><subject>Bifidobacterium</subject><subject>Bifidobacterium - isolation & purification</subject><subject>Bifidobacterium animalis subsp. lactis</subject><subject>Biomarkers - analysis</subject><subject>Breast Feeding</subject><subject>Breastfeeding & lactation</subject><subject>calprotectin</subject><subject>Cohort Studies</subject><subject>Diarrhea</subject><subject>Feces</subject><subject>Feces - chemistry</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Humans</subject><subject>Indonesia</subject><subject>Infant</subject><subject>Infant Formula - chemistry</subject><subject>Infant, Newborn</subject><subject>Intestinal Mucosa - chemistry</subject><subject>Intestinal Mucosa - microbiology</subject><subject>Leukocyte L1 Antigen Complex - analysis</subject><subject>Male</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Milk, Human</subject><subject>Polymerase Chain Reaction</subject><subject>prebiotic</subject><subject>Prebiotics</subject><subject>probiotic</subject><subject>Probiotics</subject><subject>α1-antitrypsin</subject><issn>1034-4810</issn><issn>1440-1754</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkktv1DAQxyMEog848AWQJS4cmq5fsZMjWtFSVAFSQUhcrIntqN4mdmo7gv2YfCO83aUHTvji0czvPw_NVNUrgs9JeavNrM8JZVg-qY4J57gmsuFPi40Zr3lL8FF1ktIGY0ybpn1eHVEmW0oFO65-r8M0Q3QpeBQGlHIII5qcjqF3IQPSJRySyy74dHYIwzjfAqnBZ5fjdk7OI_AG6eKPIVudi0MHr63PEQ7KHWAcxHgbLJQKIfbOuLzdFb3yJnibHHjk_FDSJjRYg_poIeXSzHiHQkTzviWnV3O0e6tOyzyPdiqFCj-EOC0jvKieDTAm-_Lwn1bfLt5_XX-orz9fXq3fXdeOEyHrobeNwY0ENnSi7XGrB20osI4NwljZ8Qa44ZRwJokVLUhhpCZCU2nBYtOx0-rtPm9p7H6xKavJJW3HEbwNS1KEd1QQKjr-H6iQgjWCkIK--QfdhCX6MsiOEozwsrtCvT5QSz9Zo-boJohb9XevBVjtgZ9utNvHOMFqdzCqHIx6OBj18cv6wSiKeq9wKdtfjwqId0pIJhv1_dOluri56VraSvWD_QG6wsd4</recordid><startdate>201312</startdate><enddate>201312</enddate><creator>Oswari, Hanifah</creator><creator>Prayitno, Lamtorogung</creator><creator>Dwipoerwantoro, Pramita G</creator><creator>Firmansyah, Agus</creator><creator>Makrides, Maria</creator><creator>Lawley, Blair</creator><creator>Kuhn-Sherlock, Barbara</creator><creator>Cleghorn, Geoffrey</creator><creator>Tannock, Gerald W</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>ASE</scope><scope>FPQ</scope><scope>K6X</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201312</creationdate><title>Comparison of stool microbiota compositions, stool alpha1-antitrypsin and calprotectin concentrations, and diarrhoeal morbidity of Indonesian infants fed breast milk or probiotic/prebiotic-supplemented formula</title><author>Oswari, Hanifah ; Prayitno, Lamtorogung ; Dwipoerwantoro, Pramita G ; Firmansyah, Agus ; Makrides, Maria ; Lawley, Blair ; Kuhn-Sherlock, Barbara ; Cleghorn, Geoffrey ; Tannock, Gerald W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i4167-fbe5d057a3f968b08cfcd2a393f6de7945a4d4214371e68a76d7c16c27eae0d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>alpha 1-Antitrypsin - analysis</topic><topic>Babies</topic><topic>Baby foods</topic><topic>Bifidobacterium</topic><topic>Bifidobacterium - isolation & purification</topic><topic>Bifidobacterium animalis subsp. lactis</topic><topic>Biomarkers - analysis</topic><topic>Breast Feeding</topic><topic>Breastfeeding & lactation</topic><topic>calprotectin</topic><topic>Cohort Studies</topic><topic>Diarrhea</topic><topic>Feces</topic><topic>Feces - chemistry</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Humans</topic><topic>Indonesia</topic><topic>Infant</topic><topic>Infant Formula - chemistry</topic><topic>Infant, Newborn</topic><topic>Intestinal Mucosa - chemistry</topic><topic>Intestinal Mucosa - microbiology</topic><topic>Leukocyte L1 Antigen Complex - analysis</topic><topic>Male</topic><topic>Microbiology</topic><topic>Microbiota</topic><topic>Milk, Human</topic><topic>Polymerase Chain Reaction</topic><topic>prebiotic</topic><topic>Prebiotics</topic><topic>probiotic</topic><topic>Probiotics</topic><topic>α1-antitrypsin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oswari, Hanifah</creatorcontrib><creatorcontrib>Prayitno, Lamtorogung</creatorcontrib><creatorcontrib>Dwipoerwantoro, Pramita G</creatorcontrib><creatorcontrib>Firmansyah, Agus</creatorcontrib><creatorcontrib>Makrides, Maria</creatorcontrib><creatorcontrib>Lawley, Blair</creatorcontrib><creatorcontrib>Kuhn-Sherlock, Barbara</creatorcontrib><creatorcontrib>Cleghorn, Geoffrey</creatorcontrib><creatorcontrib>Tannock, Gerald W</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>British Nursing Index</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>British Nursing Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of paediatrics and child health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oswari, Hanifah</au><au>Prayitno, Lamtorogung</au><au>Dwipoerwantoro, Pramita G</au><au>Firmansyah, Agus</au><au>Makrides, Maria</au><au>Lawley, Blair</au><au>Kuhn-Sherlock, Barbara</au><au>Cleghorn, Geoffrey</au><au>Tannock, Gerald W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of stool microbiota compositions, stool alpha1-antitrypsin and calprotectin concentrations, and diarrhoeal morbidity of Indonesian infants fed breast milk or probiotic/prebiotic-supplemented formula</atitle><jtitle>Journal of paediatrics and child health</jtitle><addtitle>J Paediatr Child Health</addtitle><date>2013-12</date><risdate>2013</risdate><volume>49</volume><issue>12</issue><spage>1032</spage><epage>1039</epage><pages>1032-1039</pages><issn>1034-4810</issn><eissn>1440-1754</eissn><abstract>Aim
The composition of faecal microbiota of babies is known to be influenced by diet. Faecal calprotectin and α1‐antitrypsin concentrations may be associated with mucosal permeability and inflammation. We aimed to assess whether there was any difference after consumption of a probiotic/prebiotic formula on faecal microbiota composition, calprotectin and α1‐antitrypsin levels, and diarrhoea in comparison with breast milk‐fed Indonesian infants.
Methods
One hundred sixty infants, 2 to 6 weeks old, were recruited to the study. They were either breastfed or formula fed (80 per group). Faecal samples were collected at recruitment and 3 months later. Bacterial groups characteristic of the human faecal microbiota were quantified in faeces by quantitative polymerase chain reaction. Calprotectin and α1‐antitrypsin concentrations were measured using commercial kits. Details of diarrhoeal morbidity were documented and rated for severity.
Results
The compositions of the faecal microbiota of formula‐fed compared with breast milk‐fed children were similar except that the probiotic strain Bifidobacterium animalis subsp. lactis DR10 was more abundant after 3 months consumption of the formula. Alpha1‐antitrypsin levels were higher in breastfed compared with formula‐fed infants. The occurrence of diarrhoea did not differ between the groups of babies.
Conclusion
Feeding Indonesian babies with a probiotic/prebiotic formula did not produce marked differences in the composition of the faecal microbiota in comparison with breast milk. Detrimental effects of formula feeding on biomarkers of mucosal health were not observed.</abstract><cop>Australia</cop><pub>Blackwell Publishing Ltd</pub><pmid>23782263</pmid><doi>10.1111/jpc.12307</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 1034-4810 |
| ispartof | Journal of paediatrics and child health, 2013-12, Vol.49 (12), p.1032-1039 |
| issn | 1034-4810 1440-1754 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | alpha 1-Antitrypsin - analysis Babies Baby foods Bifidobacterium Bifidobacterium - isolation & purification Bifidobacterium animalis subsp. lactis Biomarkers - analysis Breast Feeding Breastfeeding & lactation calprotectin Cohort Studies Diarrhea Feces Feces - chemistry Feces - microbiology Female Humans Indonesia Infant Infant Formula - chemistry Infant, Newborn Intestinal Mucosa - chemistry Intestinal Mucosa - microbiology Leukocyte L1 Antigen Complex - analysis Male Microbiology Microbiota Milk, Human Polymerase Chain Reaction prebiotic Prebiotics probiotic Probiotics α1-antitrypsin |
| title | Comparison of stool microbiota compositions, stool alpha1-antitrypsin and calprotectin concentrations, and diarrhoeal morbidity of Indonesian infants fed breast milk or probiotic/prebiotic-supplemented formula |
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