Shake and stew: a non-destructive PCR-ready DNA isolation method from a single preserved fish larva
A rapid non‐destructive alternative to isolate DNA from an individual fish larva is presented, based on the suspension of epithelial cells through vortex forces, and the release of DNA in a heated alkaline solution. DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR am...
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| Veröffentlicht in: | Journal of fish biology 2014-01, Vol.84 (1), p.267-272 |
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| container_end_page | 272 |
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| container_issue | 1 |
| container_start_page | 267 |
| container_title | Journal of fish biology |
| container_volume | 84 |
| creator | Alvarado Bremer, J. R. Smith, B. L. Moulton, D. L. Lu, C.-P. Cornic, M. |
| description | A rapid non‐destructive alternative to isolate DNA from an individual fish larva is presented, based on the suspension of epithelial cells through vortex forces, and the release of DNA in a heated alkaline solution. DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR amplification success rate (>93%), suggesting its applicability to other taxonomic groups or sources when tissue amount is the limiting factor. |
| doi_str_mv | 10.1111/jfb.12280 |
| format | Article |
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DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR amplification success rate (>93%), suggesting its applicability to other taxonomic groups or sources when tissue amount is the limiting factor.</description><identifier>ISSN: 0022-1112</identifier><identifier>EISSN: 1095-8649</identifier><identifier>DOI: 10.1111/jfb.12280</identifier><identifier>PMID: 24383811</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Deoxyribonucleic acid ; DNA ; DNA - isolation & purification ; Epithelial Cells ; Fishes ; genetics ; ichthyoplankton ; Istiophorus platypterus ; Larva - genetics ; Microbiology ; minimally invasive ; NaOH ; Polymerase Chain Reaction - methods ; Specimen Handling - methods ; Thunnus albacares</subject><ispartof>Journal of fish biology, 2014-01, Vol.84 (1), p.267-272</ispartof><rights>2014 The Fisheries Society of the British Isles</rights><rights>2014 The Fisheries Society of the British Isles.</rights><rights>Journal of Fish Biology © 2014 The Fisheries Society of the British Isles</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4240-3cd84ce40ad3c5a5bf790c637f958342bf945305582e5bd0b619cadbc9231bfa3</citedby><cites>FETCH-LOGICAL-c4240-3cd84ce40ad3c5a5bf790c637f958342bf945305582e5bd0b619cadbc9231bfa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfb.12280$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfb.12280$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24383811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alvarado Bremer, J. 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| fulltext | fulltext |
| identifier | ISSN: 0022-1112 |
| ispartof | Journal of fish biology, 2014-01, Vol.84 (1), p.267-272 |
| issn | 0022-1112 1095-8649 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1492612636 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Deoxyribonucleic acid DNA DNA - isolation & purification Epithelial Cells Fishes genetics ichthyoplankton Istiophorus platypterus Larva - genetics Microbiology minimally invasive NaOH Polymerase Chain Reaction - methods Specimen Handling - methods Thunnus albacares |
| title | Shake and stew: a non-destructive PCR-ready DNA isolation method from a single preserved fish larva |
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