CXCL12, CXCR4 and IFNγ genes expression: implications for proinflammatory microenvironment of breast cancer
Signals from the microenvironment have a profound influence on the maintenance or progression of breast cancer. In the present study, the frequency of CXCL12 rs1801157 polymorphism in peripheral blood and the expression of CXCL12, CXCR4 and IFNγ mRNA in normal and mammary gland tumor tissues were as...
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| Veröffentlicht in: | Clinical and experimental medicine 2013-08, Vol.13 (3), p.211-219 |
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| creator | de Oliveira, Karen Brajão Guembarovski, Roberta Losi Guembarovski, Alda Maria Fiorina Losi da Silva do Amaral Herrera, Ana Cristina Sobrinho, Walter Jorge Ariza, Carolina Batista Watanabe, Maria Angelica Ehara |
| description | Signals from the microenvironment have a profound influence on the maintenance or progression of breast cancer. In the present study, the frequency of CXCL12 rs1801157 polymorphism in peripheral blood and the expression of CXCL12, CXCR4 and IFNγ mRNA in normal and mammary gland tumor tissues were assessed in breast cancer patients. Genotyping was performed by polymerase chain reaction followed by restriction fragment length polymorphism and expression analyses by quantitative RT-PCR. A lower CXCL12 mRNA relative expression was observed among allele A carriers when compared to GG carriers (
p
= 0.012). ER-positive breast cancer allele A carriers showed a significantly lower expression of CXCL12 mRNA within tumor tissue than in normal breast tissue when compared to GG ER-positive patients (
p
= 0.016). CXCR4 mRNA (
p
|
| doi_str_mv | 10.1007/s10238-012-0194-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1492607927</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1492607927</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-ea2e173f699d612640266c7eb44620bca180d4a340ac40c1c9ce0880402cbdd53</originalsourceid><addsrcrecordid>eNqNkc1KxDAUhYMo_j-AG8nShdWbNG0adzL4B4OCKLgLaXorlTYZk47oc_kePpMZZnQpLsLN5X73cJJDyAGDEwYgTyMDnlcZMJ6OElmxRrZZoVimCl6tr-5VpWCL7MT4AsCKKodNssV5qVQp5TbpJ0-TKePHNNV7QY1r6M3l7dcnfUaHkeL7LGCMnXdntBtmfWfNmJpIWx_oLPjOtb0ZBjP68EGHzgaP7q0L3g3oRupbWgc0caTWOIthj2y0po-4v6q75PHy4mFynU3vrm4m59PM5lKOGRqOTOZt8tiUjJcCeFlaibUQJYfaGlZBI0wuwFgBllllEaoKEmfrpinyXXK01E0OX-cYRz100WLfG4d-HjUTipcgFZf_QJks8jwZSChboumVMQZs9Sx0gwkfmoFe5KGXeeiUh17koRdODlfy83rA5nfjJ4AE8CUQ08g9Y9Avfh5c-p0_VL8Bf--Vmg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1417533266</pqid></control><display><type>article</type><title>CXCL12, CXCR4 and IFNγ genes expression: implications for proinflammatory microenvironment of breast cancer</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>de Oliveira, Karen Brajão ; Guembarovski, Roberta Losi ; Guembarovski, Alda Maria Fiorina Losi ; da Silva do Amaral Herrera, Ana Cristina ; Sobrinho, Walter Jorge ; Ariza, Carolina Batista ; Watanabe, Maria Angelica Ehara</creator><creatorcontrib>de Oliveira, Karen Brajão ; Guembarovski, Roberta Losi ; Guembarovski, Alda Maria Fiorina Losi ; da Silva do Amaral Herrera, Ana Cristina ; Sobrinho, Walter Jorge ; Ariza, Carolina Batista ; Watanabe, Maria Angelica Ehara</creatorcontrib><description>Signals from the microenvironment have a profound influence on the maintenance or progression of breast cancer. In the present study, the frequency of CXCL12 rs1801157 polymorphism in peripheral blood and the expression of CXCL12, CXCR4 and IFNγ mRNA in normal and mammary gland tumor tissues were assessed in breast cancer patients. Genotyping was performed by polymerase chain reaction followed by restriction fragment length polymorphism and expression analyses by quantitative RT-PCR. A lower CXCL12 mRNA relative expression was observed among allele A carriers when compared to GG carriers (
p
= 0.012). ER-positive breast cancer allele A carriers showed a significantly lower expression of CXCL12 mRNA within tumor tissue than in normal breast tissue when compared to GG ER-positive patients (
p
= 0.016). CXCR4 mRNA (
p
< 0.001) and CXCL12 mRNA (
p
= 0.02) relative expressions were significantly correlated with relative IFN
γ
mRNA expression. Allele A carriers presenting high levels of IFN
γ
had a significantly higher expression of CXCR4 mRNA in tumor tissue than GG patients (
p
= 0.026). It is possible that allele A carrier hormone receptor–positive patients could be more susceptible to metastasis development, since they present a lower CXCL12 expression in tumor tissue, and tumor cells expressing CXCR4 could migrate toward CXCL12 gradient. IFN
γ
expression increases in order to improve immune response and could favor higher CXCR4 expression leading to migration of cells, possibly of metastatic ones, too.</description><identifier>ISSN: 1591-8890</identifier><identifier>EISSN: 1591-9528</identifier><identifier>DOI: 10.1007/s10238-012-0194-5</identifier><identifier>PMID: 22699677</identifier><language>eng</language><publisher>Milan: Springer Milan</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Breast cancer ; Breast Neoplasms - immunology ; Breast Neoplasms - pathology ; Chemokine CXCL12 - biosynthesis ; Chemokine CXCL12 - genetics ; Female ; Gene Expression ; Genotype ; Hematology ; Humans ; Interferon-gamma - biosynthesis ; Interferon-gamma - genetics ; Internal Medicine ; Medicine ; Medicine & Public Health ; Middle Aged ; Oncology ; Original Article ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Receptors, CXCR4 - biosynthesis ; Receptors, CXCR4 - genetics ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics</subject><ispartof>Clinical and experimental medicine, 2013-08, Vol.13 (3), p.211-219</ispartof><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-ea2e173f699d612640266c7eb44620bca180d4a340ac40c1c9ce0880402cbdd53</citedby><cites>FETCH-LOGICAL-c377t-ea2e173f699d612640266c7eb44620bca180d4a340ac40c1c9ce0880402cbdd53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10238-012-0194-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10238-012-0194-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22699677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Oliveira, Karen Brajão</creatorcontrib><creatorcontrib>Guembarovski, Roberta Losi</creatorcontrib><creatorcontrib>Guembarovski, Alda Maria Fiorina Losi</creatorcontrib><creatorcontrib>da Silva do Amaral Herrera, Ana Cristina</creatorcontrib><creatorcontrib>Sobrinho, Walter Jorge</creatorcontrib><creatorcontrib>Ariza, Carolina Batista</creatorcontrib><creatorcontrib>Watanabe, Maria Angelica Ehara</creatorcontrib><title>CXCL12, CXCR4 and IFNγ genes expression: implications for proinflammatory microenvironment of breast cancer</title><title>Clinical and experimental medicine</title><addtitle>Clin Exp Med</addtitle><addtitle>Clin Exp Med</addtitle><description>Signals from the microenvironment have a profound influence on the maintenance or progression of breast cancer. In the present study, the frequency of CXCL12 rs1801157 polymorphism in peripheral blood and the expression of CXCL12, CXCR4 and IFNγ mRNA in normal and mammary gland tumor tissues were assessed in breast cancer patients. Genotyping was performed by polymerase chain reaction followed by restriction fragment length polymorphism and expression analyses by quantitative RT-PCR. A lower CXCL12 mRNA relative expression was observed among allele A carriers when compared to GG carriers (
p
= 0.012). ER-positive breast cancer allele A carriers showed a significantly lower expression of CXCL12 mRNA within tumor tissue than in normal breast tissue when compared to GG ER-positive patients (
p
= 0.016). CXCR4 mRNA (
p
< 0.001) and CXCL12 mRNA (
p
= 0.02) relative expressions were significantly correlated with relative IFN
γ
mRNA expression. Allele A carriers presenting high levels of IFN
γ
had a significantly higher expression of CXCR4 mRNA in tumor tissue than GG patients (
p
= 0.026). It is possible that allele A carrier hormone receptor–positive patients could be more susceptible to metastasis development, since they present a lower CXCL12 expression in tumor tissue, and tumor cells expressing CXCR4 could migrate toward CXCL12 gradient. IFN
γ
expression increases in order to improve immune response and could favor higher CXCR4 expression leading to migration of cells, possibly of metastatic ones, too.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - immunology</subject><subject>Breast Neoplasms - pathology</subject><subject>Chemokine CXCL12 - biosynthesis</subject><subject>Chemokine CXCL12 - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Genotype</subject><subject>Hematology</subject><subject>Humans</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Interferon-gamma - genetics</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Receptors, CXCR4 - biosynthesis</subject><subject>Receptors, CXCR4 - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><issn>1591-8890</issn><issn>1591-9528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1KxDAUhYMo_j-AG8nShdWbNG0adzL4B4OCKLgLaXorlTYZk47oc_kePpMZZnQpLsLN5X73cJJDyAGDEwYgTyMDnlcZMJ6OElmxRrZZoVimCl6tr-5VpWCL7MT4AsCKKodNssV5qVQp5TbpJ0-TKePHNNV7QY1r6M3l7dcnfUaHkeL7LGCMnXdntBtmfWfNmJpIWx_oLPjOtb0ZBjP68EGHzgaP7q0L3g3oRupbWgc0caTWOIthj2y0po-4v6q75PHy4mFynU3vrm4m59PM5lKOGRqOTOZt8tiUjJcCeFlaibUQJYfaGlZBI0wuwFgBllllEaoKEmfrpinyXXK01E0OX-cYRz100WLfG4d-HjUTipcgFZf_QJks8jwZSChboumVMQZs9Sx0gwkfmoFe5KGXeeiUh17koRdODlfy83rA5nfjJ4AE8CUQ08g9Y9Avfh5c-p0_VL8Bf--Vmg</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>de Oliveira, Karen Brajão</creator><creator>Guembarovski, Roberta Losi</creator><creator>Guembarovski, Alda Maria Fiorina Losi</creator><creator>da Silva do Amaral Herrera, Ana Cristina</creator><creator>Sobrinho, Walter Jorge</creator><creator>Ariza, Carolina Batista</creator><creator>Watanabe, Maria Angelica Ehara</creator><general>Springer Milan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130801</creationdate><title>CXCL12, CXCR4 and IFNγ genes expression: implications for proinflammatory microenvironment of breast cancer</title><author>de Oliveira, Karen Brajão ; Guembarovski, Roberta Losi ; Guembarovski, Alda Maria Fiorina Losi ; da Silva do Amaral Herrera, Ana Cristina ; Sobrinho, Walter Jorge ; Ariza, Carolina Batista ; Watanabe, Maria Angelica Ehara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-ea2e173f699d612640266c7eb44620bca180d4a340ac40c1c9ce0880402cbdd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - immunology</topic><topic>Breast Neoplasms - pathology</topic><topic>Chemokine CXCL12 - biosynthesis</topic><topic>Chemokine CXCL12 - genetics</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Genotype</topic><topic>Hematology</topic><topic>Humans</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Interferon-gamma - genetics</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Receptors, CXCR4 - biosynthesis</topic><topic>Receptors, CXCR4 - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Karen Brajão</creatorcontrib><creatorcontrib>Guembarovski, Roberta Losi</creatorcontrib><creatorcontrib>Guembarovski, Alda Maria Fiorina Losi</creatorcontrib><creatorcontrib>da Silva do Amaral Herrera, Ana Cristina</creatorcontrib><creatorcontrib>Sobrinho, Walter Jorge</creatorcontrib><creatorcontrib>Ariza, Carolina Batista</creatorcontrib><creatorcontrib>Watanabe, Maria Angelica Ehara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Clinical and experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, Karen Brajão</au><au>Guembarovski, Roberta Losi</au><au>Guembarovski, Alda Maria Fiorina Losi</au><au>da Silva do Amaral Herrera, Ana Cristina</au><au>Sobrinho, Walter Jorge</au><au>Ariza, Carolina Batista</au><au>Watanabe, Maria Angelica Ehara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CXCL12, CXCR4 and IFNγ genes expression: implications for proinflammatory microenvironment of breast cancer</atitle><jtitle>Clinical and experimental medicine</jtitle><stitle>Clin Exp Med</stitle><addtitle>Clin Exp Med</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>13</volume><issue>3</issue><spage>211</spage><epage>219</epage><pages>211-219</pages><issn>1591-8890</issn><eissn>1591-9528</eissn><abstract>Signals from the microenvironment have a profound influence on the maintenance or progression of breast cancer. In the present study, the frequency of CXCL12 rs1801157 polymorphism in peripheral blood and the expression of CXCL12, CXCR4 and IFNγ mRNA in normal and mammary gland tumor tissues were assessed in breast cancer patients. Genotyping was performed by polymerase chain reaction followed by restriction fragment length polymorphism and expression analyses by quantitative RT-PCR. A lower CXCL12 mRNA relative expression was observed among allele A carriers when compared to GG carriers (
p
= 0.012). ER-positive breast cancer allele A carriers showed a significantly lower expression of CXCL12 mRNA within tumor tissue than in normal breast tissue when compared to GG ER-positive patients (
p
= 0.016). CXCR4 mRNA (
p
< 0.001) and CXCL12 mRNA (
p
= 0.02) relative expressions were significantly correlated with relative IFN
γ
mRNA expression. Allele A carriers presenting high levels of IFN
γ
had a significantly higher expression of CXCR4 mRNA in tumor tissue than GG patients (
p
= 0.026). It is possible that allele A carrier hormone receptor–positive patients could be more susceptible to metastasis development, since they present a lower CXCL12 expression in tumor tissue, and tumor cells expressing CXCR4 could migrate toward CXCL12 gradient. IFN
γ
expression increases in order to improve immune response and could favor higher CXCR4 expression leading to migration of cells, possibly of metastatic ones, too.</abstract><cop>Milan</cop><pub>Springer Milan</pub><pmid>22699677</pmid><doi>10.1007/s10238-012-0194-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1591-8890 |
| ispartof | Clinical and experimental medicine, 2013-08, Vol.13 (3), p.211-219 |
| issn | 1591-8890 1591-9528 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Aged, 80 and over Breast cancer Breast Neoplasms - immunology Breast Neoplasms - pathology Chemokine CXCL12 - biosynthesis Chemokine CXCL12 - genetics Female Gene Expression Genotype Hematology Humans Interferon-gamma - biosynthesis Interferon-gamma - genetics Internal Medicine Medicine Medicine & Public Health Middle Aged Oncology Original Article Polymerase Chain Reaction Polymorphism, Single Nucleotide Receptors, CXCR4 - biosynthesis Receptors, CXCR4 - genetics RNA, Messenger - biosynthesis RNA, Messenger - genetics |
| title | CXCL12, CXCR4 and IFNγ genes expression: implications for proinflammatory microenvironment of breast cancer |
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