Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease

Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator....

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Veröffentlicht in:	Animal reproduction science 2013-07, Vol.140 (1-2), p.14-25
Hauptverfasser:	Rodríguez, F.M, Salvetti, N.R, Colombero, M, Stangaferro, M.L, Barbeito, C.G, Ortega, H.H, Rey, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blotting, Western - veterinary Cattle Cattle - metabolism chemical oxygen demand Cystic ovarian disease Female follicular fluid Follicular Fluid - metabolism gene expression Immunohistochemistry - veterinary in situ hybridization In Situ Hybridization - veterinary Indexing in process Insulin-like growth factor insulin-like growth factor binding proteins Insulin-Like Growth Factor Binding Proteins - genetics Insulin-Like Growth Factor Binding Proteins - metabolism Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - metabolism messenger RNA ovarian cysts Ovarian Cysts - metabolism Ovarian Cysts - veterinary Ovaries polymerase chain reaction Real-Time Polymerase Chain Reaction - veterinary RNA, Messenger - chemistry RNA, Messenger - genetics somatomedins
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Zusammenfassung:	Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P
ISSN:	0378-4320 1873-2232
DOI:	10.1016/j.anireprosci.2013.04.012