Bioaccumulation of Nanosilver by Chlamydomonas reinhardtiiNanoparticle or the Free Ion?
The bioavailability of a small silver nanoparticle (nAg; nominal size of 5 nm with a polyacrylate coating) by the green alga C. reinhardtii was investigated in order to assess the contributions of Ag+ and nAg to cellular internalization. Upon exposure to nAg, Ag biouptake exceeded what was predicted...
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| Veröffentlicht in: | Environmental science & technology 2014-01, Vol.48 (1), p.358-364 |
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| creator | Leclerc, Simon Wilkinson, Kevin J |
| description | The bioavailability of a small silver nanoparticle (nAg; nominal size of 5 nm with a polyacrylate coating) by the green alga C. reinhardtii was investigated in order to assess the contributions of Ag+ and nAg to cellular internalization. Upon exposure to nAg, Ag biouptake exceeded what was predicted based upon measured Ag+ concentrations. Indeed, although Ag biouptake was greatly reduced when excess cysteine was added to the nAg, it was nonetheless significantly above control levels. For both exposures to nAg and Ag+, expression levels of the Copper Transport Protein 2 (CTR2) indicated that Ag biouptake could be attributed to the internalization of Ag+. Exposure to Ag+ or nAg increased CTR2 expression, even when cysteine was present with the nAg. Darkfield microscopy coupled with hyperspectral imagery showed that the presence of silver nanoparticles inside the cells was more likely due to the rereduction of Ag+ than to the internalization of nAg. The weight of evidence indicated that nAg increased Ag biouptake by locally increasing the surface concentrations of Ag+. |
| doi_str_mv | 10.1021/es404037z |
| format | Article |
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Upon exposure to nAg, Ag biouptake exceeded what was predicted based upon measured Ag+ concentrations. Indeed, although Ag biouptake was greatly reduced when excess cysteine was added to the nAg, it was nonetheless significantly above control levels. For both exposures to nAg and Ag+, expression levels of the Copper Transport Protein 2 (CTR2) indicated that Ag biouptake could be attributed to the internalization of Ag+. Exposure to Ag+ or nAg increased CTR2 expression, even when cysteine was present with the nAg. Darkfield microscopy coupled with hyperspectral imagery showed that the presence of silver nanoparticles inside the cells was more likely due to the rereduction of Ag+ than to the internalization of nAg. The weight of evidence indicated that nAg increased Ag biouptake by locally increasing the surface concentrations of Ag+.</description><identifier>ISSN: 0013-936X</identifier><identifier>EISSN: 1520-5851</identifier><identifier>DOI: 10.1021/es404037z</identifier><identifier>PMID: 24320028</identifier><identifier>CODEN: ESTHAG</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Algae ; Animal, plant and microbial ecology ; Applied ecology ; Biological and medical sciences ; Cation Transport Proteins - genetics ; Cells ; Chlamydomonas reinhardtii ; Chlamydomonas reinhardtii - drug effects ; Chlamydomonas reinhardtii - metabolism ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on plants and fungi ; Fundamental and applied biological sciences. Psychology ; Metal Nanoparticles - chemistry ; Nanoparticles ; Proteins ; RNA, Messenger - metabolism ; Silver - chemistry ; Silver - pharmacology ; Solubility</subject><ispartof>Environmental science & technology, 2014-01, Vol.48 (1), p.358-364</ispartof><rights>Copyright © 2013 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Jan 7, 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a406t-3b1b4ec74bcf4902b3bcdc1ba484121189ede665ea399e81e3d274c214dda1f93</citedby><cites>FETCH-LOGICAL-a406t-3b1b4ec74bcf4902b3bcdc1ba484121189ede665ea399e81e3d274c214dda1f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/es404037z$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/es404037z$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28268823$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24320028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leclerc, Simon</creatorcontrib><creatorcontrib>Wilkinson, Kevin J</creatorcontrib><title>Bioaccumulation of Nanosilver by Chlamydomonas reinhardtiiNanoparticle or the Free Ion?</title><title>Environmental science & technology</title><addtitle>Environ. Sci. Technol</addtitle><description>The bioavailability of a small silver nanoparticle (nAg; nominal size of 5 nm with a polyacrylate coating) by the green alga C. reinhardtii was investigated in order to assess the contributions of Ag+ and nAg to cellular internalization. Upon exposure to nAg, Ag biouptake exceeded what was predicted based upon measured Ag+ concentrations. Indeed, although Ag biouptake was greatly reduced when excess cysteine was added to the nAg, it was nonetheless significantly above control levels. For both exposures to nAg and Ag+, expression levels of the Copper Transport Protein 2 (CTR2) indicated that Ag biouptake could be attributed to the internalization of Ag+. Exposure to Ag+ or nAg increased CTR2 expression, even when cysteine was present with the nAg. Darkfield microscopy coupled with hyperspectral imagery showed that the presence of silver nanoparticles inside the cells was more likely due to the rereduction of Ag+ than to the internalization of nAg. The weight of evidence indicated that nAg increased Ag biouptake by locally increasing the surface concentrations of Ag+.</description><subject>Algae</subject><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Cation Transport Proteins - genetics</subject><subject>Cells</subject><subject>Chlamydomonas reinhardtii</subject><subject>Chlamydomonas reinhardtii - drug effects</subject><subject>Chlamydomonas reinhardtii - metabolism</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on plants and fungi</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles</subject><subject>Proteins</subject><subject>RNA, Messenger - metabolism</subject><subject>Silver - chemistry</subject><subject>Silver - pharmacology</subject><subject>Solubility</subject><issn>0013-936X</issn><issn>1520-5851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0c1K5TAUB_AgM-jVceELDAERnEWdfLVNVqIXHQVxNiOIm3KannIjbXMnaYXri_gqPpXPYC9ePxg3swoHfvxzkj8hO5wdcCb4T4yKKSbz-zUy4algSapT_oVMGOMyMTK73iCbMd4yxoRkep1sCCXFOOgJuTl2Hqwd2qGB3vmO-ppeQueja-4w0HJBp7MG2kXlW99BpAFdN4NQ9c49PTwu5RxC72yD1Afaz5CeBkR67rvDb-RrDU3E7dW5Ra5OT_5Mz5KL37_Op0cXCSiW9YkseanQ5qq0tTJMlLK0leUlKK244FwbrDDLUgRpDGqOshK5soKrqgJeG7lF9l9y58H_HTD2ReuixaaBDv0QC66MyNj4Len_UJZLo8UydfcfeuuH0I0PGVWeC5NLlY3qx4uywccYsC7mwbUQFgVnxbKb4q2b0X5fJQ5li9WbfC1jBHsrANFCUwforIvvTotMayHfHdj4YatPFz4DfA6i-Q</recordid><startdate>20140107</startdate><enddate>20140107</enddate><creator>Leclerc, Simon</creator><creator>Wilkinson, Kevin J</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>M7N</scope></search><sort><creationdate>20140107</creationdate><title>Bioaccumulation of Nanosilver by Chlamydomonas reinhardtiiNanoparticle or the Free Ion?</title><author>Leclerc, Simon ; Wilkinson, Kevin J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a406t-3b1b4ec74bcf4902b3bcdc1ba484121189ede665ea399e81e3d274c214dda1f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Algae</topic><topic>Animal, plant and microbial ecology</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Cation Transport Proteins - genetics</topic><topic>Cells</topic><topic>Chlamydomonas reinhardtii</topic><topic>Chlamydomonas reinhardtii - drug effects</topic><topic>Chlamydomonas reinhardtii - metabolism</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on plants and fungi</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanoparticles</topic><topic>Proteins</topic><topic>RNA, Messenger - metabolism</topic><topic>Silver - chemistry</topic><topic>Silver - pharmacology</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leclerc, Simon</creatorcontrib><creatorcontrib>Wilkinson, Kevin J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Environmental science & technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leclerc, Simon</au><au>Wilkinson, Kevin J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioaccumulation of Nanosilver by Chlamydomonas reinhardtiiNanoparticle or the Free Ion?</atitle><jtitle>Environmental science & technology</jtitle><addtitle>Environ. 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Darkfield microscopy coupled with hyperspectral imagery showed that the presence of silver nanoparticles inside the cells was more likely due to the rereduction of Ag+ than to the internalization of nAg. The weight of evidence indicated that nAg increased Ag biouptake by locally increasing the surface concentrations of Ag+.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>24320028</pmid><doi>10.1021/es404037z</doi><tpages>7</tpages></addata></record> |
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| subjects | Algae Animal, plant and microbial ecology Applied ecology Biological and medical sciences Cation Transport Proteins - genetics Cells Chlamydomonas reinhardtii Chlamydomonas reinhardtii - drug effects Chlamydomonas reinhardtii - metabolism Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on plants and fungi Fundamental and applied biological sciences. Psychology Metal Nanoparticles - chemistry Nanoparticles Proteins RNA, Messenger - metabolism Silver - chemistry Silver - pharmacology Solubility |
| title | Bioaccumulation of Nanosilver by Chlamydomonas reinhardtiiNanoparticle or the Free Ion? |
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