Transformation of cortisol to prednisolone by viable cells of Arthrobacter simplex covalently immobilized on cellulose granules
Arthrobacter simplex cells have been covalently immobilized to granules of microcrystallized regenerated cellulose by means of N-hydroxymethyl, N′-glucosylurea groups at pH 8.5, 18°C and cell suspension concentration of 60 mg/ml. The immobilization yield was found to exceed 100%. The maximum initial...
Gespeichert in:
| Veröffentlicht in: | Enzyme and microbial technology 1987, Vol.9 (9), p.538-541 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 541 |
|---|---|
| container_issue | 9 |
| container_start_page | 538 |
| container_title | Enzyme and microbial technology |
| container_volume | 9 |
| creator | Krysteva, Milka A. Grigorova, Penka M. |
| description | Arthrobacter simplex cells have been covalently immobilized to granules of microcrystallized regenerated cellulose by means of
N-hydroxymethyl,
N′-glucosylurea groups at pH 8.5, 18°C and cell suspension concentration of 60 mg/ml. The immobilization yield was found to exceed 100%. The maximum initial rate of Cortisol transformation to Prednisolone remained almost constant after 20-fold transformation in a nutrient medium containing 0.5% peptone at pH 8.0, 32°C and aeration with oxygen. The effect of the substrate concentration on the activity of the immobilized cells, as well as of the ratio between substrate and immobilized cells on the degree of transformation, was investigated. The immobilized cells were characterized by means of electronmicroscopic studies. Microbiological observations have shown that immobilized cells can proliferate and the free cells obtained are accumulated in the nutrient medium. The immobilized cells preserve their viability for a long time when they are stored at 4°C. |
| doi_str_mv | 10.1016/0141-0229(87)90083-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_14915147</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0141022987900834</els_id><sourcerecordid>14915147</sourcerecordid><originalsourceid>FETCH-LOGICAL-c400t-86c0e71c2ee5cc13e13db8f4725490aab111509204e1a79abd570c8c1282f0c33</originalsourceid><addsrcrecordid>eNp9kE-P1DAMxSMEEsPCN-CQA0JwKNhtumkuSKsV_6SVuCznKHVdCEqbIcmMGC58dVJmtUdOlq3fe7afEM8R3iDg5VtAhQ20rXk16NcGYOga9UDscNCmAQPmodjdI4_Fk5x_ANSBgp34c5vcmueYFld8XGWcJcVUfI5Blij3iad1a-LKcjzJo3djYEkcQt7Yq1S-pzg6Kpxk9ss-8K9qcHSB1xJO0i9LHH3wv3mS1X3THULMLL_VtYfA-al4NLuQ-dldvRBfP7y_vf7U3Hz5-Pn66qYhBVCa4ZKANVLL3BNhx9hN4zAr3fbKgHMjIvZgWlCMThs3Tr0GGgjboZ2Buu5CvDz77lP8eeBc7OLzdo5bOR6yRWWwR6UrqM4gpZhz4tnuk19cOlkEu6VttyjtFqUdtP2XtlVV9uLO32VyYa7vkc_3Wq2UapWp2LszxvXXo-dkM3leiSefmIqdov__nr9SlpYa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14915147</pqid></control><display><type>article</type><title>Transformation of cortisol to prednisolone by viable cells of Arthrobacter simplex covalently immobilized on cellulose granules</title><source>Elsevier ScienceDirect Journals</source><creator>Krysteva, Milka A. ; Grigorova, Penka M.</creator><creatorcontrib>Krysteva, Milka A. ; Grigorova, Penka M.</creatorcontrib><description>Arthrobacter simplex cells have been covalently immobilized to granules of microcrystallized regenerated cellulose by means of
N-hydroxymethyl,
N′-glucosylurea groups at pH 8.5, 18°C and cell suspension concentration of 60 mg/ml. The immobilization yield was found to exceed 100%. The maximum initial rate of Cortisol transformation to Prednisolone remained almost constant after 20-fold transformation in a nutrient medium containing 0.5% peptone at pH 8.0, 32°C and aeration with oxygen. The effect of the substrate concentration on the activity of the immobilized cells, as well as of the ratio between substrate and immobilized cells on the degree of transformation, was investigated. The immobilized cells were characterized by means of electronmicroscopic studies. Microbiological observations have shown that immobilized cells can proliferate and the free cells obtained are accumulated in the nutrient medium. The immobilized cells preserve their viability for a long time when they are stored at 4°C.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/0141-0229(87)90083-4</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Arthrobacter simplex ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; cellulose ; cortisone ; covalent immobilization of cells ; Fundamental and applied biological sciences. Psychology ; immobilized cells ; Methods. Procedures. Technologies ; prednisolone ; transformation of steroids ; viable immobilized cells</subject><ispartof>Enzyme and microbial technology, 1987, Vol.9 (9), p.538-541</ispartof><rights>1987</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-86c0e71c2ee5cc13e13db8f4725490aab111509204e1a79abd570c8c1282f0c33</citedby><cites>FETCH-LOGICAL-c400t-86c0e71c2ee5cc13e13db8f4725490aab111509204e1a79abd570c8c1282f0c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0141022987900834$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7444249$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Krysteva, Milka A.</creatorcontrib><creatorcontrib>Grigorova, Penka M.</creatorcontrib><title>Transformation of cortisol to prednisolone by viable cells of Arthrobacter simplex covalently immobilized on cellulose granules</title><title>Enzyme and microbial technology</title><description>Arthrobacter simplex cells have been covalently immobilized to granules of microcrystallized regenerated cellulose by means of
N-hydroxymethyl,
N′-glucosylurea groups at pH 8.5, 18°C and cell suspension concentration of 60 mg/ml. The immobilization yield was found to exceed 100%. The maximum initial rate of Cortisol transformation to Prednisolone remained almost constant after 20-fold transformation in a nutrient medium containing 0.5% peptone at pH 8.0, 32°C and aeration with oxygen. The effect of the substrate concentration on the activity of the immobilized cells, as well as of the ratio between substrate and immobilized cells on the degree of transformation, was investigated. The immobilized cells were characterized by means of electronmicroscopic studies. Microbiological observations have shown that immobilized cells can proliferate and the free cells obtained are accumulated in the nutrient medium. The immobilized cells preserve their viability for a long time when they are stored at 4°C.</description><subject>Arthrobacter simplex</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cellulose</subject><subject>cortisone</subject><subject>covalent immobilization of cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>immobilized cells</subject><subject>Methods. Procedures. Technologies</subject><subject>prednisolone</subject><subject>transformation of steroids</subject><subject>viable immobilized cells</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNp9kE-P1DAMxSMEEsPCN-CQA0JwKNhtumkuSKsV_6SVuCznKHVdCEqbIcmMGC58dVJmtUdOlq3fe7afEM8R3iDg5VtAhQ20rXk16NcGYOga9UDscNCmAQPmodjdI4_Fk5x_ANSBgp34c5vcmueYFld8XGWcJcVUfI5Blij3iad1a-LKcjzJo3djYEkcQt7Yq1S-pzg6Kpxk9ss-8K9qcHSB1xJO0i9LHH3wv3mS1X3THULMLL_VtYfA-al4NLuQ-dldvRBfP7y_vf7U3Hz5-Pn66qYhBVCa4ZKANVLL3BNhx9hN4zAr3fbKgHMjIvZgWlCMThs3Tr0GGgjboZ2Buu5CvDz77lP8eeBc7OLzdo5bOR6yRWWwR6UrqM4gpZhz4tnuk19cOlkEu6VttyjtFqUdtP2XtlVV9uLO32VyYa7vkc_3Wq2UapWp2LszxvXXo-dkM3leiSefmIqdov__nr9SlpYa</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Krysteva, Milka A.</creator><creator>Grigorova, Penka M.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>1987</creationdate><title>Transformation of cortisol to prednisolone by viable cells of Arthrobacter simplex covalently immobilized on cellulose granules</title><author>Krysteva, Milka A. ; Grigorova, Penka M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-86c0e71c2ee5cc13e13db8f4725490aab111509204e1a79abd570c8c1282f0c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Arthrobacter simplex</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cellulose</topic><topic>cortisone</topic><topic>covalent immobilization of cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>immobilized cells</topic><topic>Methods. Procedures. Technologies</topic><topic>prednisolone</topic><topic>transformation of steroids</topic><topic>viable immobilized cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krysteva, Milka A.</creatorcontrib><creatorcontrib>Grigorova, Penka M.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krysteva, Milka A.</au><au>Grigorova, Penka M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of cortisol to prednisolone by viable cells of Arthrobacter simplex covalently immobilized on cellulose granules</atitle><jtitle>Enzyme and microbial technology</jtitle><date>1987</date><risdate>1987</risdate><volume>9</volume><issue>9</issue><spage>538</spage><epage>541</epage><pages>538-541</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>Arthrobacter simplex cells have been covalently immobilized to granules of microcrystallized regenerated cellulose by means of
N-hydroxymethyl,
N′-glucosylurea groups at pH 8.5, 18°C and cell suspension concentration of 60 mg/ml. The immobilization yield was found to exceed 100%. The maximum initial rate of Cortisol transformation to Prednisolone remained almost constant after 20-fold transformation in a nutrient medium containing 0.5% peptone at pH 8.0, 32°C and aeration with oxygen. The effect of the substrate concentration on the activity of the immobilized cells, as well as of the ratio between substrate and immobilized cells on the degree of transformation, was investigated. The immobilized cells were characterized by means of electronmicroscopic studies. Microbiological observations have shown that immobilized cells can proliferate and the free cells obtained are accumulated in the nutrient medium. The immobilized cells preserve their viability for a long time when they are stored at 4°C.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/0141-0229(87)90083-4</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 1987, Vol.9 (9), p.538-541 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14915147 |
| source | Elsevier ScienceDirect Journals |
| subjects | Arthrobacter simplex Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology cellulose cortisone covalent immobilization of cells Fundamental and applied biological sciences. Psychology immobilized cells Methods. Procedures. Technologies prednisolone transformation of steroids viable immobilized cells |
| title | Transformation of cortisol to prednisolone by viable cells of Arthrobacter simplex covalently immobilized on cellulose granules |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T14%3A01%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transformation%20of%20cortisol%20to%20prednisolone%20by%20viable%20cells%20of%20Arthrobacter%20simplex%20covalently%20immobilized%20on%20cellulose%20granules&rft.jtitle=Enzyme%20and%20microbial%20technology&rft.au=Krysteva,%20Milka%20A.&rft.date=1987&rft.volume=9&rft.issue=9&rft.spage=538&rft.epage=541&rft.pages=538-541&rft.issn=0141-0229&rft.eissn=1879-0909&rft.coden=EMTED2&rft_id=info:doi/10.1016/0141-0229(87)90083-4&rft_dat=%3Cproquest_cross%3E14915147%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14915147&rft_id=info:pmid/&rft_els_id=0141022987900834&rfr_iscdi=true |