Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages
DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation....
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| Veröffentlicht in: | Gene 2014-01, Vol.534 (2), p.356-361 |
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| creator | Huang, Yong-Zhen Zhan, Zhao-Yang Sun, Yu-Jia Cao, Xiu-Kai Li, Ming-Xun Wang, Jing Lan, Xian-Yong Lei, Chu-Zhao Zhang, Chun-Lei Chen, Hong |
| description | DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation. The aim of this study was to evaluate the expression of IGF2 and the methylation pattern on the differentially methylated region (DMR) of the last exon of IGF2 in six tissues with two different developmental stages. The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The quantitative real-time PCR (qPCR) analysis indicated that IGF2 has a broad tissue distribution and the adult bovine group showed significant lower mRNA expression levels than that in the fetal bovine group (P |
| doi_str_mv | 10.1016/j.gene.2013.09.111 |
| format | Article |
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•The IGF2 plays an important role in muscle growth.•The appropriate DNA methylation is essential for normal gene function.•The adult bovine exhibited a lower mRNA level and higher DNA methylation level than the fetal bovine during in six organs and tissues.•A useful molecular marker for muscle developmental and as a model for studies in other species.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2013.09.111</identifier><identifier>PMID: 24140490</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Breeding ; Cattle ; DMR ; DNA Methylation ; Down-Regulation ; Exons ; Gene Expression ; IGF2 ; Insulin-Like Growth Factor II - genetics ; Male ; Molecular Sequence Data ; Muscle Development - genetics ; qPCR ; RNA, Messenger - genetics ; Tissue Distribution</subject><ispartof>Gene, 2014-01, Vol.534 (2), p.356-361</ispartof><rights>2013</rights><rights>2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-d8b398eb5b46d84847cc4de10e78bb46a2917d720c4784e09cad09eb3909c713</citedby><cites>FETCH-LOGICAL-c356t-d8b398eb5b46d84847cc4de10e78bb46a2917d720c4784e09cad09eb3909c713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378111913013450$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24140490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yong-Zhen</creatorcontrib><creatorcontrib>Zhan, Zhao-Yang</creatorcontrib><creatorcontrib>Sun, Yu-Jia</creatorcontrib><creatorcontrib>Cao, Xiu-Kai</creatorcontrib><creatorcontrib>Li, Ming-Xun</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Lan, Xian-Yong</creatorcontrib><creatorcontrib>Lei, Chu-Zhao</creatorcontrib><creatorcontrib>Zhang, Chun-Lei</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><title>Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages</title><title>Gene</title><addtitle>Gene</addtitle><description>DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation. The aim of this study was to evaluate the expression of IGF2 and the methylation pattern on the differentially methylated region (DMR) of the last exon of IGF2 in six tissues with two different developmental stages. The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The quantitative real-time PCR (qPCR) analysis indicated that IGF2 has a broad tissue distribution and the adult bovine group showed significant lower mRNA expression levels than that in the fetal bovine group (P<0.05 or P<0.01). Moreover, the DNA methylation level analysis showed that the adult bovine group exhibited a significantly higher DNA methylation levels than that in the fetal bovine group (P<0.05 or P<0.01). These results indicate that IGF2 expression levels were negatively associated with the methylation status of the IGF2 DMR during the two developmental stages. Our results suggest that the methylation pattern in this DMR may be a useful parameter to investigate as a marker-assisted selection for muscle developmental in beef cattle breeding program and as a model for studies in other species.
•The IGF2 plays an important role in muscle growth.•The appropriate DNA methylation is essential for normal gene function.•The adult bovine exhibited a lower mRNA level and higher DNA methylation level than the fetal bovine during in six organs and tissues.•A useful molecular marker for muscle developmental and as a model for studies in other species.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Breeding</subject><subject>Cattle</subject><subject>DMR</subject><subject>DNA Methylation</subject><subject>Down-Regulation</subject><subject>Exons</subject><subject>Gene Expression</subject><subject>IGF2</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Muscle Development - genetics</subject><subject>qPCR</subject><subject>RNA, Messenger - genetics</subject><subject>Tissue Distribution</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UMtu2zAQJIoWjZP2B3ooeOxFylKiTRLIJcjTQNBecicocu3SkCiXpJzk70PBaY7Zyy4GM7OYIeQHg5oBW53v6i0GrBtgbQ2qZox9IgsmhaoAWvmZLKAVsiqwOiGnKe2gzHLZfCUnDWccuIIFCeuQoyk-3tLr35d0wPz3pTfZj4GmbPKUqBufQhVxOxUYE-3Ggw9I13e3DZ3_U3zeR0xpVvhAnd9sMGLI1OEB-3E_lNv0s9kW0zfyZWP6hN_f9hl5vL15vLqvHv7cra8uHyrbLle5crJrlcRu2fGVk1xyYS13yACF7ApmGsWEEw1YLiRHUNY4UFhE5RSsPSO_jrb7OP6bMGU9-GSx703AcUqalexCKOBQqM2RauOYUsSN3kc_mPiiGei5Zr3Tc0w916xB6dJnEf1885-6Ad275H-vhXBxJGAJefAYdbIeg0XnI9qs3eg_8n8F-y2PnA</recordid><startdate>20140125</startdate><enddate>20140125</enddate><creator>Huang, Yong-Zhen</creator><creator>Zhan, Zhao-Yang</creator><creator>Sun, Yu-Jia</creator><creator>Cao, Xiu-Kai</creator><creator>Li, Ming-Xun</creator><creator>Wang, Jing</creator><creator>Lan, Xian-Yong</creator><creator>Lei, Chu-Zhao</creator><creator>Zhang, Chun-Lei</creator><creator>Chen, Hong</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140125</creationdate><title>Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages</title><author>Huang, Yong-Zhen ; Zhan, Zhao-Yang ; Sun, Yu-Jia ; Cao, Xiu-Kai ; Li, Ming-Xun ; Wang, Jing ; Lan, Xian-Yong ; Lei, Chu-Zhao ; Zhang, Chun-Lei ; Chen, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-d8b398eb5b46d84847cc4de10e78bb46a2917d720c4784e09cad09eb3909c713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Breeding</topic><topic>Cattle</topic><topic>DMR</topic><topic>DNA Methylation</topic><topic>Down-Regulation</topic><topic>Exons</topic><topic>Gene Expression</topic><topic>IGF2</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Muscle Development - genetics</topic><topic>qPCR</topic><topic>RNA, Messenger - genetics</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yong-Zhen</creatorcontrib><creatorcontrib>Zhan, Zhao-Yang</creatorcontrib><creatorcontrib>Sun, Yu-Jia</creatorcontrib><creatorcontrib>Cao, Xiu-Kai</creatorcontrib><creatorcontrib>Li, Ming-Xun</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Lan, Xian-Yong</creatorcontrib><creatorcontrib>Lei, Chu-Zhao</creatorcontrib><creatorcontrib>Zhang, Chun-Lei</creatorcontrib><creatorcontrib>Chen, Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yong-Zhen</au><au>Zhan, Zhao-Yang</au><au>Sun, Yu-Jia</au><au>Cao, Xiu-Kai</au><au>Li, Ming-Xun</au><au>Wang, Jing</au><au>Lan, Xian-Yong</au><au>Lei, Chu-Zhao</au><au>Zhang, Chun-Lei</au><au>Chen, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2014-01-25</date><risdate>2014</risdate><volume>534</volume><issue>2</issue><spage>356</spage><epage>361</epage><pages>356-361</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation. The aim of this study was to evaluate the expression of IGF2 and the methylation pattern on the differentially methylated region (DMR) of the last exon of IGF2 in six tissues with two different developmental stages. The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The quantitative real-time PCR (qPCR) analysis indicated that IGF2 has a broad tissue distribution and the adult bovine group showed significant lower mRNA expression levels than that in the fetal bovine group (P<0.05 or P<0.01). Moreover, the DNA methylation level analysis showed that the adult bovine group exhibited a significantly higher DNA methylation levels than that in the fetal bovine group (P<0.05 or P<0.01). These results indicate that IGF2 expression levels were negatively associated with the methylation status of the IGF2 DMR during the two developmental stages. Our results suggest that the methylation pattern in this DMR may be a useful parameter to investigate as a marker-assisted selection for muscle developmental in beef cattle breeding program and as a model for studies in other species.
•The IGF2 plays an important role in muscle growth.•The appropriate DNA methylation is essential for normal gene function.•The adult bovine exhibited a lower mRNA level and higher DNA methylation level than the fetal bovine during in six organs and tissues.•A useful molecular marker for muscle developmental and as a model for studies in other species.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24140490</pmid><doi>10.1016/j.gene.2013.09.111</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Base Sequence Breeding Cattle DMR DNA Methylation Down-Regulation Exons Gene Expression IGF2 Insulin-Like Growth Factor II - genetics Male Molecular Sequence Data Muscle Development - genetics qPCR RNA, Messenger - genetics Tissue Distribution |
| title | Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages |
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