Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR
Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002; Doleans et al. in J Clin Microbiol 42:458–460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008). In this study, methodologies based on molecular biology were...
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| Veröffentlicht in: | Archives of microbiology 2014-01, Vol.196 (1), p.63-71 |
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| creator | Grúas, Cristina Llambi, Silvia Arruga, M. Victoria |
| description | Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002; Doleans et al. in J Clin Microbiol 42:458–460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene. |
| doi_str_mv | 10.1007/s00203-013-0934-2 |
| format | Article |
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Victoria</creator><creatorcontrib>Grúas, Cristina ; Llambi, Silvia ; Arruga, M. Victoria</creatorcontrib><description>Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002; Doleans et al. in J Clin Microbiol 42:458–460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-013-0934-2</identifier><identifier>PMID: 24264468</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Bacteria ; Bacterial Proteins - genetics ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Ecology ; Environmental Monitoring - methods ; gene amplification ; Genes ; Humans ; Legionella - genetics ; Legionella - isolation & purification ; Legionella - physiology ; Legionella pneumophila ; Legionella pneumophila - genetics ; Legionella pneumophila - isolation & purification ; Legionella pneumophila - physiology ; Life Sciences ; macrophages ; Methods ; Microbial Ecology ; Microbiology ; Molecular biology ; pathogenicity ; Peptidylprolyl Isomerase - genetics ; Polymerase chain reaction ; quantitative polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; Sensitivity and Specificity ; Short Communication ; Spain ; Water analysis ; Water Microbiology ; Water sampling</subject><ispartof>Archives of microbiology, 2014-01, Vol.196 (1), p.63-71</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-2a1af035755743540dedc5f631ea5cf2e131ac0c1a077c318c9a23d7c3005f203</citedby><cites>FETCH-LOGICAL-c466t-2a1af035755743540dedc5f631ea5cf2e131ac0c1a077c318c9a23d7c3005f203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-013-0934-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-013-0934-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24264468$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grúas, Cristina</creatorcontrib><creatorcontrib>Llambi, Silvia</creatorcontrib><creatorcontrib>Arruga, M. Victoria</creatorcontrib><title>Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Legionella pneumophila is the primary cause of the legionellosis diseases (90 %) (Yu et al. in J Infect Dis 186:127–128, 2002; Doleans et al. in J Clin Microbiol 42:458–460, 2004; Den Boer et al. in Clin Microbiol Infect 14:459–466, 2008). In this study, methodologies based on molecular biology were developed in order to provide a quick diagnosis of the bacterial presence in water samples of Spain. Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.</description><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Ecology</subject><subject>Environmental Monitoring - methods</subject><subject>gene amplification</subject><subject>Genes</subject><subject>Humans</subject><subject>Legionella - genetics</subject><subject>Legionella - isolation & purification</subject><subject>Legionella - physiology</subject><subject>Legionella pneumophila</subject><subject>Legionella pneumophila - genetics</subject><subject>Legionella pneumophila - isolation & purification</subject><subject>Legionella pneumophila - physiology</subject><subject>Life Sciences</subject><subject>macrophages</subject><subject>Methods</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Molecular biology</subject><subject>pathogenicity</subject><subject>Peptidylprolyl Isomerase - genetics</subject><subject>Polymerase chain reaction</subject><subject>quantitative polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Short Communication</subject><subject>Spain</subject><subject>Water analysis</subject><subject>Water Microbiology</subject><subject>Water sampling</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtv1TAQhS1ERS-FH8AGIrFhkzLjR-K7RJendKUiSiV2luuMLy551U6E-u_rkFJVLFhYHo2_c2zPYewFwikC1G8TAAdRAua1FbLkj9gGpeAl1PzHY7YBAbzUWyGO2dOUrgCQa62fsGMueSVlpTfs13uayE1h6IvBF3s65Ira1hZpHE8L2zcPe2NPczeMP0OuQ1_8thPFItlubCkt8vPR5vblTRaTCz64IpJtyyl0VHzdfXvGjrxtEz2_20_YxccP33efy_3Zpy-7d_vSyaqaSm7RehCqVqqWQkloqHHKVwLJKuc5oUDrwKGFunYCtdtaLppcAiifB3LC3qy-YxyuZ0qT6UJyyw96GuZkUG6hrlBLntHX_6BXwxz7_LpM1Qp4Vf2hcKVcHFKK5M0YQ2fjjUEwSxJmTcLkJMyShFk0L--c58uOmnvF39FngK9Aykf9geKDq__j-moVeTsYe4ghmYtzDqgyiUKDFrfO5Jsw</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Grúas, Cristina</creator><creator>Llambi, Silvia</creator><creator>Arruga, M. 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Multiplex real-time polymerase chain reaction assays were realized to target the 16S rRNA and macrophage infectivity potentiator (mip) genes of, respectively, Legionella spp. and L. pneumophila including in the design of an internal control. The results obtained by the culture and the gene amplification methods agreed in 94.44 % for the 16S rRNA gene, and a concordance of 66.67 % of the cases was obtained for the mip gene.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>24264468</pmid><doi>10.1007/s00203-013-0934-2</doi><tpages>9</tpages></addata></record> |
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| subjects | Bacteria Bacterial Proteins - genetics Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Ecology Environmental Monitoring - methods gene amplification Genes Humans Legionella - genetics Legionella - isolation & purification Legionella - physiology Legionella pneumophila Legionella pneumophila - genetics Legionella pneumophila - isolation & purification Legionella pneumophila - physiology Life Sciences macrophages Methods Microbial Ecology Microbiology Molecular biology pathogenicity Peptidylprolyl Isomerase - genetics Polymerase chain reaction quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction ribosomal RNA RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Short Communication Spain Water analysis Water Microbiology Water sampling |
| title | Detection of Legionella spp. and Legionella pneumophila in water samples of Spain by specific real-time PCR |
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