Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection
Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable o...
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| Veröffentlicht in: | Analytical biochemistry 2014-02, Vol.446, p.9-18 |
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| creator | Canbaz, Mehmet Çetin Sezgintürk, Mustafa Kemal |
| description | Anti-HER-3 antibody was used for the first time in a disposable immunosensor based on indium tin oxide (ITO) substrate for HER-3 quantification. Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (Rct) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the “single frequency impedance” technique was first used for characterization of interaction between HER-3 and anti-HER-3. Eventually, the proposed ITO-based immunosensor was applied to artificial serum samples spiked with HER-3. |
| doi_str_mv | 10.1016/j.ab.2013.10.014 |
| format | Article |
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Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (Rct) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the “single frequency impedance” technique was first used for characterization of interaction between HER-3 and anti-HER-3. 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Anti-HER-3 was immobilized onto ITO substrate by 3-aminopropyl)triethoxysilane (APTES) and glutaraldehyde. This highly sensitive immunosensor was capable of detecting concentrations of HER-3 down to the femtogram/ml level by investigating changes in the charge transfer resistance (Rct) using electrochemical impedance spectroscopy (EIS). Construction of ITO layers was carefully investigated using a broad range of techniques such as voltammetry, EIS, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Meanwhile, in an immunosensor system, the “single frequency impedance” technique was first used for characterization of interaction between HER-3 and anti-HER-3. 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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Antibodies, Immobilized - immunology Biomarkers, Tumor - analysis Biomarkers, Tumor - chemistry Biomarkers, Tumor - immunology Biosensing Techniques - instrumentation Biosensor Cancer biomarkers Disposable Equipment Electrodes HER-3 Immunoassay - instrumentation ITO Receptor, ErbB-3 - analysis Receptor, ErbB-3 - chemistry Receptor, ErbB-3 - immunology Single frequency impedance Surface Properties Tin Compounds - chemistry |
| title | Fabrication of a highly sensitive disposable immunosensor based on indium tin oxide substrates for cancer biomarker detection |
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