Pulse-Chase Analysis to Measure Protein Degradation
In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [35S]-methionine and [35S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). The decay of the amount o...
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| creator | Simon, Einav Kornitzer, Daniel |
| description | In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [35S]-methionine and [35S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). The decay of the amount of protein labeled during the pulse period is measured during the chase with cold precursor. |
| doi_str_mv | 10.1016/B978-0-12-420070-8.00006-4 |
| format | Book Chapter |
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The decay of the amount of protein labeled during the pulse period is measured during the chase with cold precursor.</description><identifier>ISSN: 0076-6879</identifier><identifier>ISBN: 0124200702</identifier><identifier>ISBN: 9780124200708</identifier><identifier>EISSN: 1557-7988</identifier><identifier>EISBN: 0124200974</identifier><identifier>EISBN: 9780124200975</identifier><identifier>DOI: 10.1016/B978-0-12-420070-8.00006-4</identifier><identifier>PMID: 24423267</identifier><language>eng</language><publisher>United States: Elsevier Science & Technology</publisher><subject>Amino acids ; Candida albicans - metabolism ; Cellular proteins ; Cytoplasmic pools ; Derivation of protein half-life ; Half-Life ; Immunoprecipitation ; Isotope Labeling ; Methionine - metabolism ; Methionine pulse-chase ; Protein degradation ; Proteolysis ; Publishing industry ; Pulse-chase analysis ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - metabolism</subject><ispartof>Methods in Enzymology, 2014, Vol.536, p.65-75</ispartof><rights>2014 Elsevier Inc.</rights><rights>2014 Elsevier Inc. 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The decay of the amount of protein labeled during the pulse period is measured during the chase with cold precursor.</description><subject>Amino acids</subject><subject>Candida albicans - metabolism</subject><subject>Cellular proteins</subject><subject>Cytoplasmic pools</subject><subject>Derivation of protein half-life</subject><subject>Half-Life</subject><subject>Immunoprecipitation</subject><subject>Isotope Labeling</subject><subject>Methionine - metabolism</subject><subject>Methionine pulse-chase</subject><subject>Protein degradation</subject><subject>Proteolysis</subject><subject>Publishing industry</subject><subject>Pulse-chase analysis</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>0124200702</isbn><isbn>9780124200708</isbn><isbn>0124200974</isbn><isbn>9780124200975</isbn><fulltext>true</fulltext><rsrctype>book_chapter</rsrctype><creationdate>2014</creationdate><recordtype>book_chapter</recordtype><sourceid>EIF</sourceid><recordid>eNo9UctO5TAMzTzQcAf4ARajitVswthJmscSLvOSQLCAdZTbuhDU20LSIvH3pLrMeOPHObZsH8ZOEE4RUP84d8Zy4Ci4EgAGuD2FYpqrD-wroFiqzqiPbIV1bbhx1n76DxgQn9mqeM21NW6fHeX8WLoRjdXSfmH7QikhhTYrJm_mPhNfP4RM1dkQ-tccczWN1RWFPCeqbtI4URyqC7pPoQ1THIdDtteF0nX07g_Y3a-ft-s__PL699_12SVvpLIT7zbYCSc12LqTAsm5sqpuNo0wUIsumE1na4u1blpqVdkMhZadsegQJZGSB-z7bu5TGp9nypPfxtxQ34eBxjl7VA4MgnBQqN_eqfNmS61_SnEb0qv_d2ghHO8I96En_5J6bwBqXL5iC3ixA6lc8xIp-dxEGhpqY6Jm8u0YPYJflPGLMr7Ewu9e7Uu2KOOVfAN3znYg</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Simon, Einav</creator><creator>Kornitzer, Daniel</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2014</creationdate><title>Pulse-Chase Analysis to Measure Protein Degradation</title><author>Simon, Einav ; Kornitzer, Daniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-fb1f2936085f321e995576cbc27052fa7bf858156cded48631263f7819113ee43</frbrgroupid><rsrctype>book_chapters</rsrctype><prefilter>book_chapters</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino acids</topic><topic>Candida albicans - metabolism</topic><topic>Cellular proteins</topic><topic>Cytoplasmic pools</topic><topic>Derivation of protein half-life</topic><topic>Half-Life</topic><topic>Immunoprecipitation</topic><topic>Isotope Labeling</topic><topic>Methionine - metabolism</topic><topic>Methionine pulse-chase</topic><topic>Protein degradation</topic><topic>Proteolysis</topic><topic>Publishing industry</topic><topic>Pulse-chase analysis</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon, Einav</creatorcontrib><creatorcontrib>Kornitzer, Daniel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon, Einav</au><au>Kornitzer, Daniel</au><format>book</format><genre>bookitem</genre><ristype>CHAP</ristype><atitle>Pulse-Chase Analysis to Measure Protein Degradation</atitle><btitle>Methods in Enzymology</btitle><addtitle>Methods Enzymol</addtitle><date>2014</date><risdate>2014</risdate><volume>536</volume><spage>65</spage><epage>75</epage><pages>65-75</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>0124200702</isbn><isbn>9780124200708</isbn><eisbn>0124200974</eisbn><eisbn>9780124200975</eisbn><abstract>In order to measure protein half-life by pulse-chase analysis, cells are incubated with labeled precursors, [35S]-methionine and [35S]-cysteine, for a short time (pulse), after which they are washed from the labeled compound and incubated with excess cold precursor (chase). 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| fulltext | fulltext |
| identifier | ISSN: 0076-6879 |
| ispartof | Methods in Enzymology, 2014, Vol.536, p.65-75 |
| issn | 0076-6879 1557-7988 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1490710290 |
| source | ScienceDirect eBooks; MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino acids Candida albicans - metabolism Cellular proteins Cytoplasmic pools Derivation of protein half-life Half-Life Immunoprecipitation Isotope Labeling Methionine - metabolism Methionine pulse-chase Protein degradation Proteolysis Publishing industry Pulse-chase analysis Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - metabolism |
| title | Pulse-Chase Analysis to Measure Protein Degradation |
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