The Isolation and some Properties of a Lectin (Haemagglutinin) from Cucurbita Phloem Exudate
The occurrence of high haemagglutinating (lectin) activity in phloem exudate from three cucurbit species is reported. The protein responsible for this lectin activity in Cucurbita maxima Duch. has been isolated by cation exchange chromatography on Sepharose and identified by gel electrophoresis. The...
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| Veröffentlicht in: | Planta 1978-01, Vol.142 (1), p.97-101 |
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| container_title | Planta |
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| creator | Sabnis, D.D. Hart, J.W. |
| description | The occurrence of high haemagglutinating (lectin) activity in phloem exudate from three cucurbit species is reported. The protein responsible for this lectin activity in Cucurbita maxima Duch. has been isolated by cation exchange chromatography on Sepharose and identified by gel electrophoresis. The lectin showed agglutinating activity at concentrations as low as 0.1 μg/ml. No sugar, including those transported in the phloem of these species, interacted with agglutination. The lectin could not be extracted from cucurbit seed, but appeared in 5-day old seedlings. The possible role of a lectin in the sieve element is discussed. |
| doi_str_mv | 10.1007/bf00385126 |
| format | Article |
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The protein responsible for this lectin activity in Cucurbita maxima Duch. has been isolated by cation exchange chromatography on Sepharose and identified by gel electrophoresis. The lectin showed agglutinating activity at concentrations as low as 0.1 μg/ml. No sugar, including those transported in the phloem of these species, interacted with agglutination. The lectin could not be extracted from cucurbit seed, but appeared in 5-day old seedlings. The possible role of a lectin in the sieve element is discussed.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/bf00385126</identifier><identifier>PMID: 24408004</identifier><language>eng</language><publisher>Germany: Springer-Verlag</publisher><subject>Agglutination ; Electrophoresis ; Erythrocytes ; Gels ; Lectins ; Phloem ; Reagents ; Sieve elements ; Sieve tubes ; Sugars</subject><ispartof>Planta, 1978-01, Vol.142 (1), p.97-101</ispartof><rights>Springer-Verlag Berlin Heidelberg 1978</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-f7ae005449e491c2bbe31b17e6d98f6eac671ac8c289dda51bfdb43b0cb975e33</citedby><cites>FETCH-LOGICAL-c373t-f7ae005449e491c2bbe31b17e6d98f6eac671ac8c289dda51bfdb43b0cb975e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23373498$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23373498$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,4009,27902,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24408004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sabnis, D.D.</creatorcontrib><creatorcontrib>Hart, J.W.</creatorcontrib><title>The Isolation and some Properties of a Lectin (Haemagglutinin) from Cucurbita Phloem Exudate</title><title>Planta</title><addtitle>Planta</addtitle><description>The occurrence of high haemagglutinating (lectin) activity in phloem exudate from three cucurbit species is reported. The protein responsible for this lectin activity in Cucurbita maxima Duch. has been isolated by cation exchange chromatography on Sepharose and identified by gel electrophoresis. The lectin showed agglutinating activity at concentrations as low as 0.1 μg/ml. No sugar, including those transported in the phloem of these species, interacted with agglutination. The lectin could not be extracted from cucurbit seed, but appeared in 5-day old seedlings. The possible role of a lectin in the sieve element is discussed.</description><subject>Agglutination</subject><subject>Electrophoresis</subject><subject>Erythrocytes</subject><subject>Gels</subject><subject>Lectins</subject><subject>Phloem</subject><subject>Reagents</subject><subject>Sieve elements</subject><subject>Sieve tubes</subject><subject>Sugars</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><recordid>eNpFkMFLwzAUh4Mobk4v3pUcp1B9adKmPerY3GDgDvMmlCR93TraZiYt6H9vZVNPj_d-Hz94HyHXDB4YgHzUBQBPIhbGJ2TIBA-DEERySob9OQwg5dGAXHi_A-hDKc_JIBQCEgAxJO_rLdKFt5VqS9tQ1eTU2xrpytk9urZET21BFV2iacuGjucKa7XZVF2_lc0dLZyt6aQzndNlq-hqW1ms6fSzy1WLl-SsUJXHq-MckbfZdD2ZB8vXl8XkaRkYLnkbFFIhQCREiiJlJtQaOdNMYpynSRGjMrFkyiQmTNI8VxHTRa4F12B0KiPkfETGh969sx8d-jarS2-wqlSDtvMZEylICCOe9Oj9ATXOeu-wyPaurJX7yhhkPzaz59mvzR6-PfZ2usb8D_3V1wM3B2DnW-v-c97_JdKEfwNTg3jQ</recordid><startdate>19780101</startdate><enddate>19780101</enddate><creator>Sabnis, D.D.</creator><creator>Hart, J.W.</creator><general>Springer-Verlag</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19780101</creationdate><title>The Isolation and some Properties of a Lectin (Haemagglutinin) from Cucurbita Phloem Exudate</title><author>Sabnis, D.D. ; Hart, J.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-f7ae005449e491c2bbe31b17e6d98f6eac671ac8c289dda51bfdb43b0cb975e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Agglutination</topic><topic>Electrophoresis</topic><topic>Erythrocytes</topic><topic>Gels</topic><topic>Lectins</topic><topic>Phloem</topic><topic>Reagents</topic><topic>Sieve elements</topic><topic>Sieve tubes</topic><topic>Sugars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabnis, D.D.</creatorcontrib><creatorcontrib>Hart, J.W.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabnis, D.D.</au><au>Hart, J.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Isolation and some Properties of a Lectin (Haemagglutinin) from Cucurbita Phloem Exudate</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>1978-01-01</date><risdate>1978</risdate><volume>142</volume><issue>1</issue><spage>97</spage><epage>101</epage><pages>97-101</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><abstract>The occurrence of high haemagglutinating (lectin) activity in phloem exudate from three cucurbit species is reported. The protein responsible for this lectin activity in Cucurbita maxima Duch. has been isolated by cation exchange chromatography on Sepharose and identified by gel electrophoresis. The lectin showed agglutinating activity at concentrations as low as 0.1 μg/ml. No sugar, including those transported in the phloem of these species, interacted with agglutination. The lectin could not be extracted from cucurbit seed, but appeared in 5-day old seedlings. The possible role of a lectin in the sieve element is discussed.</abstract><cop>Germany</cop><pub>Springer-Verlag</pub><pmid>24408004</pmid><doi>10.1007/bf00385126</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1978-01, Vol.142 (1), p.97-101 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1490702538 |
| source | Jstor Complete Legacy; Springer Nature - Complete Springer Journals |
| subjects | Agglutination Electrophoresis Erythrocytes Gels Lectins Phloem Reagents Sieve elements Sieve tubes Sugars |
| title | The Isolation and some Properties of a Lectin (Haemagglutinin) from Cucurbita Phloem Exudate |
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