Two types of PS II centres as manifested by light saturation of delayed fluorescence from DCMU-treated chloroplasts

Intensity of 2 s delayed fluorescence (DF) as a function of steady-state actinic light intensity was investigated in pea chloroplasts in the presence of 10 μM DCMU. The light saturation curve of DF was approximated by a sum of two hyperbolic components which differ by an order of magnitude in the ha...

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Veröffentlicht in:Photosynthesis research 1990-11, Vol.26 (2), p.101-107
Hauptverfasser: VASIL'EV, I. R, KOMAROV, A. I, MATORIN, D. N, VERKHOTUROV, V. N, VENEDIKTOV, P. S
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container_end_page 107
container_issue 2
container_start_page 101
container_title Photosynthesis research
container_volume 26
creator VASIL'EV, I. R
KOMAROV, A. I
MATORIN, D. N
VERKHOTUROV, V. N
VENEDIKTOV, P. S
description Intensity of 2 s delayed fluorescence (DF) as a function of steady-state actinic light intensity was investigated in pea chloroplasts in the presence of 10 μM DCMU. The light saturation curve of DF was approximated by a sum of two hyperbolic components which differ by an order of magnitude in the half-saturating incident light intensity. The relative contribution of the amplitudes of the components was practically independent of cation (Na(+) and Mg(2+)) concentration and a short-term heating of the chloroplasts at 45°C. The component saturating at low incident light intensity was selectively suppressed by 100 μM DCMU or by 1 μmol μg(-1) Chl oleic acid. DF intensity following excitation by a single saturating 15 μs flash was equal to the intensity of the component saturating at a low incident light intensity. Upon flash excitation, the maximum steady-state DF level was found to be attained only after a series of saturating flashes. It is concluded that the two components of the DF light saturation curves are related to PS II centres heterogeneity in quantum yield of stabilization of the reduced primary quinone acceptor.
doi_str_mv 10.1007/BF00047081
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subjects Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Metabolism
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
title Two types of PS II centres as manifested by light saturation of delayed fluorescence from DCMU-treated chloroplasts
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