Oxo-Type Organophosphate-Resistant Acetylcholinesterase from Organophosphate-Unsusceptible Culex tritaeniorhynchus
A highly resistant strain of Culex tritaeniorhynchus was employed to clarify the mechanisms of the acquired resistance of mosquitoes to organophosphorus and carbamate insecticides by an enzymological approach. Carboxylesterase (CE) activity was higher in the abdomen than in the head and thorax, and...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 1988/01/25, Vol.36(1), pp.312-315 |
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| creator | WATANABE, MAMORU TAKEBE, SACHIKO KIM, DONG-HYUN ARAKAWA, RYO KAMIMURA, KIYOSHI KOBASHI, KYOICHI |
| description | A highly resistant strain of Culex tritaeniorhynchus was employed to clarify the mechanisms of the acquired resistance of mosquitoes to organophosphorus and carbamate insecticides by an enzymological approach. Carboxylesterase (CE) activity was higher in the abdomen than in the head and thorax, and was higher in every part of the body of the resistant strain as compared with the susceptible strain. Acetylcholinesterase (AChE) activity was high in the head, and was higher in every part of the body in the susceptible strain as compared with the resistant strain.Greater inhibition of CE by oxo-type organophosphorus insecticides was observed in the susceptible strain (11-, 8.2- and 22.3-fold greater inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. Much stronger inhibition of AChE was observed in the susceptible strain (1227-, 76.5- and 183-fold inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. These results suggest that both AChE and CE are involved in the development of the acquired resistance to organophosphorus and carbamate insecticides, and that the former enzyme plays the major role. |
| doi_str_mv | 10.1248/cpb.36.312 |
| format | Article |
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Carboxylesterase (CE) activity was higher in the abdomen than in the head and thorax, and was higher in every part of the body of the resistant strain as compared with the susceptible strain. Acetylcholinesterase (AChE) activity was high in the head, and was higher in every part of the body in the susceptible strain as compared with the resistant strain.Greater inhibition of CE by oxo-type organophosphorus insecticides was observed in the susceptible strain (11-, 8.2- and 22.3-fold greater inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. Much stronger inhibition of AChE was observed in the susceptible strain (1227-, 76.5- and 183-fold inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. These results suggest that both AChE and CE are involved in the development of the acquired resistance to organophosphorus and carbamate insecticides, and that the former enzyme plays the major role.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.36.312</identifier><identifier>PMID: 3378294</identifier><identifier>CODEN: CPBTAL</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Acetylcholinesterase - metabolism ; Animals ; Biological and medical sciences ; Cholinesterase Inhibitors - pharmacology ; Culex - metabolism ; Culex tritaeniorhynchus ; Culicidae ; Fundamental and applied biological sciences. Psychology ; Insecta ; Insecticide Resistance ; Insecticides - pharmacology ; Invertebrates ; Organophosphorus Compounds ; oxo-type organophosphate inhibition ; Physiology. Development</subject><ispartof>Chemical and Pharmaceutical Bulletin, 1988/01/25, Vol.36(1), pp.312-315</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1989 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1988</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c662t-84b3ecfb24078b75246f2284ff814a86a848e8428f78706dc22bc07f30af890e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7314901$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3378294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WATANABE, MAMORU</creatorcontrib><creatorcontrib>TAKEBE, SACHIKO</creatorcontrib><creatorcontrib>KIM, DONG-HYUN</creatorcontrib><creatorcontrib>ARAKAWA, RYO</creatorcontrib><creatorcontrib>KAMIMURA, KIYOSHI</creatorcontrib><creatorcontrib>KOBASHI, KYOICHI</creatorcontrib><title>Oxo-Type Organophosphate-Resistant Acetylcholinesterase from Organophosphate-Unsusceptible Culex tritaeniorhynchus</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>A highly resistant strain of Culex tritaeniorhynchus was employed to clarify the mechanisms of the acquired resistance of mosquitoes to organophosphorus and carbamate insecticides by an enzymological approach. Carboxylesterase (CE) activity was higher in the abdomen than in the head and thorax, and was higher in every part of the body of the resistant strain as compared with the susceptible strain. Acetylcholinesterase (AChE) activity was high in the head, and was higher in every part of the body in the susceptible strain as compared with the resistant strain.Greater inhibition of CE by oxo-type organophosphorus insecticides was observed in the susceptible strain (11-, 8.2- and 22.3-fold greater inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. Much stronger inhibition of AChE was observed in the susceptible strain (1227-, 76.5- and 183-fold inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. These results suggest that both AChE and CE are involved in the development of the acquired resistance to organophosphorus and carbamate insecticides, and that the former enzyme plays the major role.</description><subject>Acetylcholinesterase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cholinesterase Inhibitors - pharmacology</subject><subject>Culex - metabolism</subject><subject>Culex tritaeniorhynchus</subject><subject>Culicidae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Insecta</subject><subject>Insecticide Resistance</subject><subject>Insecticides - pharmacology</subject><subject>Invertebrates</subject><subject>Organophosphorus Compounds</subject><subject>oxo-type organophosphate inhibition</subject><subject>Physiology. Development</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkc1r3DAQxUVoSLdpL7kXDC09FLzVlyX5GJZ-BAILJTkLWRnFXrySK8mQ_e-jZc0W2oNGh_ebmcc8hG4IXhPK1Tc7dWsm1ozQC7QijMu6oZS9QSuMcVtTJthb9C6lHca0wZJdoSvGpKItX6G4fQn1w2GCahufjQ9TH9LUmwz1b0hDysbn6tZCPoy2D-PgIWWIJkHlYtj_1_Po05wsTHnoRqg28wgvVY5DNuCHEPuDt_2c3qNLZ8YEH5b_Gj3--P6w-VXfb3_ebW7vaysEzbXiHQPrOsqxVJ1sKBeOUsWdU4QbJYziChSnykklsXiylHYWS8ewcarFwK7Rl9PcKYY_czGu90MxN47GQ5iTJrzFTUNFAT_9A-7CHH3xVhiBCVflFerribIxpBTB6SkOexMPmmB9jEGXGDQTusRQ4I_LyLnbw9MZXe5e9M-LbpI1o4vG2yGdMcmO5kjBNidsV5J4hrNuYh7sCMeNpG3UcStZCqF_1d5EDZ69AjPYqYQ</recordid><startdate>19880101</startdate><enddate>19880101</enddate><creator>WATANABE, MAMORU</creator><creator>TAKEBE, SACHIKO</creator><creator>KIM, DONG-HYUN</creator><creator>ARAKAWA, RYO</creator><creator>KAMIMURA, KIYOSHI</creator><creator>KOBASHI, KYOICHI</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7SS</scope></search><sort><creationdate>19880101</creationdate><title>Oxo-Type Organophosphate-Resistant Acetylcholinesterase from Organophosphate-Unsusceptible Culex tritaeniorhynchus</title><author>WATANABE, MAMORU ; TAKEBE, SACHIKO ; KIM, DONG-HYUN ; ARAKAWA, RYO ; KAMIMURA, KIYOSHI ; KOBASHI, KYOICHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c662t-84b3ecfb24078b75246f2284ff814a86a848e8428f78706dc22bc07f30af890e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acetylcholinesterase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cholinesterase Inhibitors - pharmacology</topic><topic>Culex - metabolism</topic><topic>Culex tritaeniorhynchus</topic><topic>Culicidae</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Insecta</topic><topic>Insecticide Resistance</topic><topic>Insecticides - pharmacology</topic><topic>Invertebrates</topic><topic>Organophosphorus Compounds</topic><topic>oxo-type organophosphate inhibition</topic><topic>Physiology. Development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WATANABE, MAMORU</creatorcontrib><creatorcontrib>TAKEBE, SACHIKO</creatorcontrib><creatorcontrib>KIM, DONG-HYUN</creatorcontrib><creatorcontrib>ARAKAWA, RYO</creatorcontrib><creatorcontrib>KAMIMURA, KIYOSHI</creatorcontrib><creatorcontrib>KOBASHI, KYOICHI</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WATANABE, MAMORU</au><au>TAKEBE, SACHIKO</au><au>KIM, DONG-HYUN</au><au>ARAKAWA, RYO</au><au>KAMIMURA, KIYOSHI</au><au>KOBASHI, KYOICHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oxo-Type Organophosphate-Resistant Acetylcholinesterase from Organophosphate-Unsusceptible Culex tritaeniorhynchus</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1988-01-01</date><risdate>1988</risdate><volume>36</volume><issue>1</issue><spage>312</spage><epage>315</epage><pages>312-315</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><coden>CPBTAL</coden><abstract>A highly resistant strain of Culex tritaeniorhynchus was employed to clarify the mechanisms of the acquired resistance of mosquitoes to organophosphorus and carbamate insecticides by an enzymological approach. Carboxylesterase (CE) activity was higher in the abdomen than in the head and thorax, and was higher in every part of the body of the resistant strain as compared with the susceptible strain. Acetylcholinesterase (AChE) activity was high in the head, and was higher in every part of the body in the susceptible strain as compared with the resistant strain.Greater inhibition of CE by oxo-type organophosphorus insecticides was observed in the susceptible strain (11-, 8.2- and 22.3-fold greater inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. Much stronger inhibition of AChE was observed in the susceptible strain (1227-, 76.5- and 183-fold inhibitions by fenitrooxon, malaoxon and dichlorvos, respectively) than in the resistant strain. These results suggest that both AChE and CE are involved in the development of the acquired resistance to organophosphorus and carbamate insecticides, and that the former enzyme plays the major role.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>3378294</pmid><doi>10.1248/cpb.36.312</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chemical and Pharmaceutical Bulletin, 1988/01/25, Vol.36(1), pp.312-315 |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Acetylcholinesterase - metabolism Animals Biological and medical sciences Cholinesterase Inhibitors - pharmacology Culex - metabolism Culex tritaeniorhynchus Culicidae Fundamental and applied biological sciences. Psychology Insecta Insecticide Resistance Insecticides - pharmacology Invertebrates Organophosphorus Compounds oxo-type organophosphate inhibition Physiology. Development |
| title | Oxo-Type Organophosphate-Resistant Acetylcholinesterase from Organophosphate-Unsusceptible Culex tritaeniorhynchus |
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