Production of amylase and cellulase: Degradation of starch and carboxymethylcellulose by extracellular enzymes from four fungal species

Four fungal species, Aspergillus terreus (SA 562), Fusarium moniliforme (SA 1190), Aspergillus carneus (SA 1326) and Phoma sorghina (SA 1397) have been screened for the production of cellulase (EC 3.2.1.4) and amylase (EC 3.2.1.1). All the species grew profusely on culture plates containing 1% groun...

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Veröffentlicht in:Enzyme and microbial technology 1987, Vol.9 (5), p.308-312
Hauptverfasser: Shambe, Tseaa, Ejembi, Onah
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description Four fungal species, Aspergillus terreus (SA 562), Fusarium moniliforme (SA 1190), Aspergillus carneus (SA 1326) and Phoma sorghina (SA 1397) have been screened for the production of cellulase (EC 3.2.1.4) and amylase (EC 3.2.1.1). All the species grew profusely on culture plates containing 1% ground millet, starch or carboxymethylcellulose (CMC) as the carbon source. Maximum growth was observed within about 96 h. When grown in submerged liquid culture, stationary or shaken at 20 strokes min −1, the species produced a good yield of extracellular cellulase and amylase. Differences in yield of enzymes for stationary and shaking growth were negligible. A preliminary study of the action of the crude enzymes on CMC, starch, ground millet (Pannisetum typhoidees) and acha (Digitaria exilis) is reported.
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All the species grew profusely on culture plates containing 1% ground millet, starch or carboxymethylcellulose (CMC) as the carbon source. Maximum growth was observed within about 96 h. When grown in submerged liquid culture, stationary or shaken at 20 strokes min −1, the species produced a good yield of extracellular cellulase and amylase. Differences in yield of enzymes for stationary and shaking growth were negligible. 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All the species grew profusely on culture plates containing 1% ground millet, starch or carboxymethylcellulose (CMC) as the carbon source. Maximum growth was observed within about 96 h. When grown in submerged liquid culture, stationary or shaken at 20 strokes min −1, the species produced a good yield of extracellular cellulase and amylase. Differences in yield of enzymes for stationary and shaking growth were negligible. A preliminary study of the action of the crude enzymes on CMC, starch, ground millet (Pannisetum typhoidees) and acha (Digitaria exilis) is reported.</description><subject>alpha -amylase</subject><subject>Aspergillus carneus</subject><subject>Aspergillus terreus</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>carboxymethylcellulose</subject><subject>cellulase</subject><subject>Cellulase production</subject><subject>Enzyme engineering</subject><subject>extracellular fungal enzymes</subject><subject>Fermentation</subject><subject>Food additives</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusarium moniliforme</subject><subject>General aspects</subject><subject>Methods. Procedures. 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source Elsevier ScienceDirect Journals Complete
subjects alpha -amylase
Aspergillus carneus
Aspergillus terreus
Biological and medical sciences
Biotechnology
carboxymethylcellulose
cellulase
Cellulase production
Enzyme engineering
extracellular fungal enzymes
Fermentation
Food additives
Food industries
Fundamental and applied biological sciences. Psychology
Fusarium moniliforme
General aspects
Methods. Procedures. Technologies
Phoma sorghina
Production of selected enzymes
starch
α-amylase
title Production of amylase and cellulase: Degradation of starch and carboxymethylcellulose by extracellular enzymes from four fungal species
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