Molecular characterization of the virulence gene virA of the Agrobacterium tumefaciens octopine Ti plasmid
The virulence loci play an essential role in tumor formation by Agrobacterium tumefaciens. Induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci virA and virG. This study focused on the virA locus of the octopine type Ti plasmid pTi15955. The nucleic ac...
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| Veröffentlicht in: | Plant molecular biology 1987-11, Vol.9 (6), p.635-645 |
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| container_title | Plant molecular biology |
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| creator | Melchers, L.S Thompson, D.V Idler, K.B Neuteboom, S.T.C Maagd, R.A. de Schilperoort, R.A Hooykaas, P.J.J |
| description | The virulence loci play an essential role in tumor formation by Agrobacterium tumefaciens. Induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci virA and virG. This study focused on the virA locus of the octopine type Ti plasmid pTi15955. The nucleic acid sequence of a 5.7-kilobase fragment encompassing virA was determined. Genetic analysis of this region revealed that virA contains one open reading frame coding for a protein of 91 639 daltons. Immunodetection with antibodies raised against a 35-kDa VirA fusion protein produced in E. coli identified by the VirA product in wild-type Agrobacterium cells. Moreover, it is shown that the VirA protein is located in the cytoplasmic membrane fraction of Agrobacterium. These data confirm the proposed regulatory function of VirA whereby VirA acts as a membrane sensor protein to identify plant signal molecules in the environment. The proposed sensory function of VirA strikingly resembles the function of the chemotaxis receptor proteins of E. coli. |
| doi_str_mv | 10.1007/BF00020539 |
| format | Article |
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Induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci virA and virG. This study focused on the virA locus of the octopine type Ti plasmid pTi15955. The nucleic acid sequence of a 5.7-kilobase fragment encompassing virA was determined. Genetic analysis of this region revealed that virA contains one open reading frame coding for a protein of 91 639 daltons. Immunodetection with antibodies raised against a 35-kDa VirA fusion protein produced in E. coli identified by the VirA product in wild-type Agrobacterium cells. Moreover, it is shown that the VirA protein is located in the cytoplasmic membrane fraction of Agrobacterium. These data confirm the proposed regulatory function of VirA whereby VirA acts as a membrane sensor protein to identify plant signal molecules in the environment. The proposed sensory function of VirA strikingly resembles the function of the chemotaxis receptor proteins of E. coli.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00020539</identifier><identifier>PMID: 24277199</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Agrobacterium tumefaciens ; amino acid sequence ; amino acid sequences ; Bryophyllum tubiflorum ; cell membranes ; crown galls ; DNA ; gene products ; genes ; genetic complementation ; immunochemistry ; molecular sequence data ; neoplasms ; Nicotiana ; Nicotiana glauca ; nucleotide sequence ; nucleotide sequences ; plasmids ; single cell protein ; Solanum lycopersicum var. lycopersicum ; virg protein ; virulence</subject><ispartof>Plant molecular biology, 1987-11, Vol.9 (6), p.635-645</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-544d752accc36907dd532734a65e3e690f617eeb15288c968451368f9b88eb413</citedby><cites>FETCH-LOGICAL-c340t-544d752accc36907dd532734a65e3e690f617eeb15288c968451368f9b88eb413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24277199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Melchers, L.S</creatorcontrib><creatorcontrib>Thompson, D.V</creatorcontrib><creatorcontrib>Idler, K.B</creatorcontrib><creatorcontrib>Neuteboom, S.T.C</creatorcontrib><creatorcontrib>Maagd, R.A. de</creatorcontrib><creatorcontrib>Schilperoort, R.A</creatorcontrib><creatorcontrib>Hooykaas, P.J.J</creatorcontrib><title>Molecular characterization of the virulence gene virA of the Agrobacterium tumefaciens octopine Ti plasmid</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The virulence loci play an essential role in tumor formation by Agrobacterium tumefaciens. Induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci virA and virG. This study focused on the virA locus of the octopine type Ti plasmid pTi15955. The nucleic acid sequence of a 5.7-kilobase fragment encompassing virA was determined. Genetic analysis of this region revealed that virA contains one open reading frame coding for a protein of 91 639 daltons. Immunodetection with antibodies raised against a 35-kDa VirA fusion protein produced in E. coli identified by the VirA product in wild-type Agrobacterium cells. Moreover, it is shown that the VirA protein is located in the cytoplasmic membrane fraction of Agrobacterium. These data confirm the proposed regulatory function of VirA whereby VirA acts as a membrane sensor protein to identify plant signal molecules in the environment. The proposed sensory function of VirA strikingly resembles the function of the chemotaxis receptor proteins of E. coli.</description><subject>Agrobacterium tumefaciens</subject><subject>amino acid sequence</subject><subject>amino acid sequences</subject><subject>Bryophyllum tubiflorum</subject><subject>cell membranes</subject><subject>crown galls</subject><subject>DNA</subject><subject>gene products</subject><subject>genes</subject><subject>genetic complementation</subject><subject>immunochemistry</subject><subject>molecular sequence data</subject><subject>neoplasms</subject><subject>Nicotiana</subject><subject>Nicotiana glauca</subject><subject>nucleotide sequence</subject><subject>nucleotide sequences</subject><subject>plasmids</subject><subject>single cell protein</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>virg protein</subject><subject>virulence</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqF0c1O3DAQAGALFbHLwqUP0OZUVZUC43_nuKwKRdqKA3COHGeyGCXx1k6Q2qcnZXfbIydrPN-MNDOEfKRwQQH05dU1ADCQvDgicyo1zyUw84HMgSqdC0HZjJym9Awwca5OyIwJpjUtijl5_hladGNrY-aebLRuwOj_2MGHPgtNNjxh9uLj2GLvMNtg_xYuD6nlJoZqVzN22TB22FjnsU9ZcEPY-ok_-Gzb2tT5-owcN7ZNeL5_F-Tx-vvD6ke-vru5XS3XueMChlwKUWvJrHOOqwJ0XUvONBdWSeQ4_TSKasSKSmaMK5QRknJlmqIyBitB-YJ82fXdxvBrxDSUnU8O29b2GMZUUmF4IbSZ4Nd3oGLUFNSoiX7bURdDShGbcht9Z-PvkkL59wjl_yNM-NO-71h1WP-jh61P4PMONDaUdhN9Kh_vGVAOVAuAaZ5XFqSKOA</recordid><startdate>198711</startdate><enddate>198711</enddate><creator>Melchers, L.S</creator><creator>Thompson, D.V</creator><creator>Idler, K.B</creator><creator>Neuteboom, S.T.C</creator><creator>Maagd, R.A. de</creator><creator>Schilperoort, R.A</creator><creator>Hooykaas, P.J.J</creator><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>198711</creationdate><title>Molecular characterization of the virulence gene virA of the Agrobacterium tumefaciens octopine Ti plasmid</title><author>Melchers, L.S ; Thompson, D.V ; Idler, K.B ; Neuteboom, S.T.C ; Maagd, R.A. de ; Schilperoort, R.A ; Hooykaas, P.J.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-544d752accc36907dd532734a65e3e690f617eeb15288c968451368f9b88eb413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Agrobacterium tumefaciens</topic><topic>amino acid sequence</topic><topic>amino acid sequences</topic><topic>Bryophyllum tubiflorum</topic><topic>cell membranes</topic><topic>crown galls</topic><topic>DNA</topic><topic>gene products</topic><topic>genes</topic><topic>genetic complementation</topic><topic>immunochemistry</topic><topic>molecular sequence data</topic><topic>neoplasms</topic><topic>Nicotiana</topic><topic>Nicotiana glauca</topic><topic>nucleotide sequence</topic><topic>nucleotide sequences</topic><topic>plasmids</topic><topic>single cell protein</topic><topic>Solanum lycopersicum var. lycopersicum</topic><topic>virg protein</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Melchers, L.S</creatorcontrib><creatorcontrib>Thompson, D.V</creatorcontrib><creatorcontrib>Idler, K.B</creatorcontrib><creatorcontrib>Neuteboom, S.T.C</creatorcontrib><creatorcontrib>Maagd, R.A. de</creatorcontrib><creatorcontrib>Schilperoort, R.A</creatorcontrib><creatorcontrib>Hooykaas, P.J.J</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Melchers, L.S</au><au>Thompson, D.V</au><au>Idler, K.B</au><au>Neuteboom, S.T.C</au><au>Maagd, R.A. de</au><au>Schilperoort, R.A</au><au>Hooykaas, P.J.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of the virulence gene virA of the Agrobacterium tumefaciens octopine Ti plasmid</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1987-11</date><risdate>1987</risdate><volume>9</volume><issue>6</issue><spage>635</spage><epage>645</epage><pages>635-645</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>The virulence loci play an essential role in tumor formation by Agrobacterium tumefaciens. Induction of vir gene expression by plant signal molecules is solely dependent on the virulence loci virA and virG. This study focused on the virA locus of the octopine type Ti plasmid pTi15955. The nucleic acid sequence of a 5.7-kilobase fragment encompassing virA was determined. Genetic analysis of this region revealed that virA contains one open reading frame coding for a protein of 91 639 daltons. Immunodetection with antibodies raised against a 35-kDa VirA fusion protein produced in E. coli identified by the VirA product in wild-type Agrobacterium cells. Moreover, it is shown that the VirA protein is located in the cytoplasmic membrane fraction of Agrobacterium. These data confirm the proposed regulatory function of VirA whereby VirA acts as a membrane sensor protein to identify plant signal molecules in the environment. The proposed sensory function of VirA strikingly resembles the function of the chemotaxis receptor proteins of E. coli.</abstract><cop>Netherlands</cop><pmid>24277199</pmid><doi>10.1007/BF00020539</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1987-11, Vol.9 (6), p.635-645 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | SpringerLink Journals |
| subjects | Agrobacterium tumefaciens amino acid sequence amino acid sequences Bryophyllum tubiflorum cell membranes crown galls DNA gene products genes genetic complementation immunochemistry molecular sequence data neoplasms Nicotiana Nicotiana glauca nucleotide sequence nucleotide sequences plasmids single cell protein Solanum lycopersicum var. lycopersicum virg protein virulence |
| title | Molecular characterization of the virulence gene virA of the Agrobacterium tumefaciens octopine Ti plasmid |
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