Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum)
The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer regi...
Gespeichert in:
| Veröffentlicht in: | Plant molecular biology 1987-03, Vol.9 (2), p.87-95 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 95 |
|---|---|
| container_issue | 2 |
| container_start_page | 87 |
| container_title | Plant molecular biology |
| container_volume | 9 |
| creator | Van't Hof, J Hernandez, P Bjerknes, C.A Lamm, S.S |
| description | The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells. |
| doi_str_mv | 10.1007/BF00015641 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_14831939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>14831939</sourcerecordid><originalsourceid>FETCH-LOGICAL-c369t-8a927f9b1d614ce3f61757185703edf7862ce156be992f40433d2355b673cd1c3</originalsourceid><addsrcrecordid>eNqF0F1rFDEUBuAgFrutvfEH6FyU0gpjz0ky-bjsd4VFBe31kMkkNTqzs01mhPrrm2G39VIIBPI-OSQvIe8QPiGAPD2_BgCsBMdXZIGVZGUFVL0mC0AhS86R7pK9lH5lBMDEG7JLOZVCabUg9XKwZgzDqhh8Mf50RXTrLjwfxXAfVkVec6LL380cOzMWKfx1he1MSvO9ePnlbFY5Ko6_hTT1RcoT_kz9yVuy402X3MF23yd311c_Lm7L5debzxdny9IyocdSGU2l1w22Arl1zAuUlURVSWCu9VIJal3-YuO0pp4DZ6ylrKoaIZlt0bJ9crSZu47Dw-TSWPchWdd1ZuWGKdXIFUPNdIbH_4GCos41qUw_bqiNQ0rR-XodQ2_iY41Qz83X_5rP-P127tT0rn2hz1VncLgFJlnT-WhWNqQXJ7kC0PP7PmyYN0Nt7mMmd98pIAOUHCqp2ROTPZCS</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1462190368</pqid></control><display><type>article</type><title>Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum)</title><source>Springer Nature - Complete Springer Journals</source><creator>Van't Hof, J ; Hernandez, P ; Bjerknes, C.A ; Lamm, S.S</creator><creatorcontrib>Van't Hof, J ; Hernandez, P ; Bjerknes, C.A ; Lamm, S.S</creatorcontrib><description>The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00015641</identifier><identifier>PMID: 24276898</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; cell synchronization ; cellulose ; chromosome mapping ; derivatives ; diethylaminoethyl cellulose ; DNA replication ; estrus synchronization ; Fundamental and applied biological sciences. Psychology ; Generalities ; Generalities. Genetics. Plant material ; genes ; Genetics and breeding of economic plants ; initiation ; ion exchange chromatography ; Molecular and cellular biology ; Molecular genetics ; non-transcribed spacer region ; Pisum sativum ; repetitive sequences ; Replication ; restriction mapping ; ribosomal DNA ; ribosomal RNA ; root meristems ; root tips</subject><ispartof>Plant molecular biology, 1987-03, Vol.9 (2), p.87-95</ispartof><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-8a927f9b1d614ce3f61757185703edf7862ce156be992f40433d2355b673cd1c3</citedby><cites>FETCH-LOGICAL-c369t-8a927f9b1d614ce3f61757185703edf7862ce156be992f40433d2355b673cd1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7480099$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24276898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Van't Hof, J</creatorcontrib><creatorcontrib>Hernandez, P</creatorcontrib><creatorcontrib>Bjerknes, C.A</creatorcontrib><creatorcontrib>Lamm, S.S</creatorcontrib><title>Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum)</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>cell synchronization</subject><subject>cellulose</subject><subject>chromosome mapping</subject><subject>derivatives</subject><subject>diethylaminoethyl cellulose</subject><subject>DNA replication</subject><subject>estrus synchronization</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities</subject><subject>Generalities. Genetics. Plant material</subject><subject>genes</subject><subject>Genetics and breeding of economic plants</subject><subject>initiation</subject><subject>ion exchange chromatography</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>non-transcribed spacer region</subject><subject>Pisum sativum</subject><subject>repetitive sequences</subject><subject>Replication</subject><subject>restriction mapping</subject><subject>ribosomal DNA</subject><subject>ribosomal RNA</subject><subject>root meristems</subject><subject>root tips</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqF0F1rFDEUBuAgFrutvfEH6FyU0gpjz0ky-bjsd4VFBe31kMkkNTqzs01mhPrrm2G39VIIBPI-OSQvIe8QPiGAPD2_BgCsBMdXZIGVZGUFVL0mC0AhS86R7pK9lH5lBMDEG7JLOZVCabUg9XKwZgzDqhh8Mf50RXTrLjwfxXAfVkVec6LL380cOzMWKfx1he1MSvO9ePnlbFY5Ko6_hTT1RcoT_kz9yVuy402X3MF23yd311c_Lm7L5debzxdny9IyocdSGU2l1w22Arl1zAuUlURVSWCu9VIJal3-YuO0pp4DZ6ylrKoaIZlt0bJ9crSZu47Dw-TSWPchWdd1ZuWGKdXIFUPNdIbH_4GCos41qUw_bqiNQ0rR-XodQ2_iY41Qz83X_5rP-P127tT0rn2hz1VncLgFJlnT-WhWNqQXJ7kC0PP7PmyYN0Nt7mMmd98pIAOUHCqp2ROTPZCS</recordid><startdate>198703</startdate><enddate>198703</enddate><creator>Van't Hof, J</creator><creator>Hernandez, P</creator><creator>Bjerknes, C.A</creator><creator>Lamm, S.S</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>198703</creationdate><title>Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum)</title><author>Van't Hof, J ; Hernandez, P ; Bjerknes, C.A ; Lamm, S.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-8a927f9b1d614ce3f61757185703edf7862ce156be992f40433d2355b673cd1c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>cell synchronization</topic><topic>cellulose</topic><topic>chromosome mapping</topic><topic>derivatives</topic><topic>diethylaminoethyl cellulose</topic><topic>DNA replication</topic><topic>estrus synchronization</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities</topic><topic>Generalities. Genetics. Plant material</topic><topic>genes</topic><topic>Genetics and breeding of economic plants</topic><topic>initiation</topic><topic>ion exchange chromatography</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>non-transcribed spacer region</topic><topic>Pisum sativum</topic><topic>repetitive sequences</topic><topic>Replication</topic><topic>restriction mapping</topic><topic>ribosomal DNA</topic><topic>ribosomal RNA</topic><topic>root meristems</topic><topic>root tips</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van't Hof, J</creatorcontrib><creatorcontrib>Hernandez, P</creatorcontrib><creatorcontrib>Bjerknes, C.A</creatorcontrib><creatorcontrib>Lamm, S.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van't Hof, J</au><au>Hernandez, P</au><au>Bjerknes, C.A</au><au>Lamm, S.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum)</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1987-03</date><risdate>1987</risdate><volume>9</volume><issue>2</issue><spage>87</spage><epage>95</epage><pages>87-95</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>The replication origin of the 9-kb rDNA repeat size class of pea (Pisum sativum cv. Alaska) was identified by benzoylated naphthoylated DEAE-cellulose column chromatography and Southern blotting procedures. The origin is located at or near a 0.19-kb EcoR I fragment in the non-transcribed spacer region between the 25S and 18S rRNA genes. Identification of the origin was based on three criteria: (i) an enrichment of the 0.19-kb fragment in replicating rDNA from asynchronously dividing root meristematic cells, (ii) the scarcity of the 0.19-kb fragment in rDNA from non-dividing carbohydrate starved cells, and (iii) a 60-min periodic enrichment of the 0.19-kb fragment in replicating rDNA that temporally coincides with the sequential initiation of replication of replicon families in synchronized pea root cells.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>24276898</pmid><doi>10.1007/BF00015641</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1987-03, Vol.9 (2), p.87-95 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14831939 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences cell synchronization cellulose chromosome mapping derivatives diethylaminoethyl cellulose DNA replication estrus synchronization Fundamental and applied biological sciences. Psychology Generalities Generalities. Genetics. Plant material genes Genetics and breeding of economic plants initiation ion exchange chromatography Molecular and cellular biology Molecular genetics non-transcribed spacer region Pisum sativum repetitive sequences Replication restriction mapping ribosomal DNA ribosomal RNA root meristems root tips |
| title | Location of the replication origin in the 9-kb repeat size class of rDNA in pea (Pisum sativum) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T10%3A24%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Location%20of%20the%20replication%20origin%20in%20the%209-kb%20repeat%20size%20class%20of%20rDNA%20in%20pea%20(Pisum%20sativum)&rft.jtitle=Plant%20molecular%20biology&rft.au=Van't%20Hof,%20J&rft.date=1987-03&rft.volume=9&rft.issue=2&rft.spage=87&rft.epage=95&rft.pages=87-95&rft.issn=0167-4412&rft.eissn=1573-5028&rft.coden=PMBIDB&rft_id=info:doi/10.1007/BF00015641&rft_dat=%3Cproquest_cross%3E14831939%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1462190368&rft_id=info:pmid/24276898&rfr_iscdi=true |