Hyperproduction of aspartase of Escherichia coli K-12 by the use of a runaway plasmid vector

Plasmid pYT125 bearing the aspartase gene ( aspA) of Escherichia coli K-12 was constructed by the use of pSY343, runaway-replication plasmid vector. One of the transformants, strain MM294 (pYT125)-S maintained pYT125 stably at 37°C in a non-selective medium. This strain produced approx. 60-fold more...

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Veröffentlicht in:	Journal of biotechnology 1987, Vol.6 (1), p.31-40
Hauptverfasser:	Nishimura, Noriyuki, Komatsubara, Saburo, Taniguchi, Tomoyasu, Kisumi, Masahiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspartase aspartate ammonia-lyase Escherichia coli genes Plasmid stability plasmids Runaway-replication-plasmid
Online-Zugang:	Volltext
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