METHANOL ACCUMULATION BY RESTING CELLS OF METHYLOSINUS TRICHOSPORIUM (I)
Techniques for measuring methanol accumulated by resting cells of Methylosinus trichosporium in the narrow range of 70-80mM phosphate concentration have been standardized. The maximum amount of methanol, 6μmol•mg-1•hr-1, was accumulated at a cell density of 2mg•ml-1 (w/v) of cells in the early stati...
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| Veröffentlicht in: | Journal of general and applied microbiology 1987, Vol.33(3), pp.221-229 |
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| container_title | Journal of general and applied microbiology |
| container_volume | 33 |
| creator | MEHTA, PERDEEP K. MISHRA, SAROJ GHOSE, TARUN K. |
| description | Techniques for measuring methanol accumulated by resting cells of Methylosinus trichosporium in the narrow range of 70-80mM phosphate concentration have been standardized. The maximum amount of methanol, 6μmol•mg-1•hr-1, was accumulated at a cell density of 2mg•ml-1 (w/v) of cells in the early stationary phase at pH 6.5, temperature 35°C. The phosphate-dependent inhibition of methanol dehydrogenase was found to be uncompetitive and reversible. Further, 80mM phosphate inhibited methane monooxygenase and formate dehydrogenase by 16% and 20% respectively. A possible biochemical explanation of the role of phosphate inhibition is offered. |
| doi_str_mv | 10.2323/jgam.33.221 |
| format | Article |
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The maximum amount of methanol, 6μmol•mg-1•hr-1, was accumulated at a cell density of 2mg•ml-1 (w/v) of cells in the early stationary phase at pH 6.5, temperature 35°C. The phosphate-dependent inhibition of methanol dehydrogenase was found to be uncompetitive and reversible. Further, 80mM phosphate inhibited methane monooxygenase and formate dehydrogenase by 16% and 20% respectively. A possible biochemical explanation of the role of phosphate inhibition is offered.</description><identifier>ISSN: 0022-1260</identifier><identifier>EISSN: 1349-8037</identifier><identifier>DOI: 10.2323/jgam.33.221</identifier><identifier>CODEN: JGAMA9</identifier><language>eng</language><publisher>Tokyo: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</publisher><subject>Bacteriology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Metabolism. 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Gen. Appl. Microbiol.</addtitle><description>Techniques for measuring methanol accumulated by resting cells of Methylosinus trichosporium in the narrow range of 70-80mM phosphate concentration have been standardized. The maximum amount of methanol, 6μmol•mg-1•hr-1, was accumulated at a cell density of 2mg•ml-1 (w/v) of cells in the early stationary phase at pH 6.5, temperature 35°C. The phosphate-dependent inhibition of methanol dehydrogenase was found to be uncompetitive and reversible. Further, 80mM phosphate inhibited methane monooxygenase and formate dehydrogenase by 16% and 20% respectively. A possible biochemical explanation of the role of phosphate inhibition is offered.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism. 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Psychology</topic><topic>Metabolism. Enzymes</topic><topic>Methylosinus trichosporium</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Physiology and metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MEHTA, PERDEEP K.</creatorcontrib><creatorcontrib>MISHRA, SAROJ</creatorcontrib><creatorcontrib>GHOSE, TARUN K.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of general and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MEHTA, PERDEEP K.</au><au>MISHRA, SAROJ</au><au>GHOSE, TARUN K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>METHANOL ACCUMULATION BY RESTING CELLS OF METHYLOSINUS TRICHOSPORIUM (I)</atitle><jtitle>Journal of general and applied microbiology</jtitle><addtitle>J. Gen. Appl. Microbiol.</addtitle><date>1987</date><risdate>1987</risdate><volume>33</volume><issue>3</issue><spage>221</spage><epage>229</epage><pages>221-229</pages><issn>0022-1260</issn><eissn>1349-8037</eissn><coden>JGAMA9</coden><abstract>Techniques for measuring methanol accumulated by resting cells of Methylosinus trichosporium in the narrow range of 70-80mM phosphate concentration have been standardized. The maximum amount of methanol, 6μmol•mg-1•hr-1, was accumulated at a cell density of 2mg•ml-1 (w/v) of cells in the early stationary phase at pH 6.5, temperature 35°C. The phosphate-dependent inhibition of methanol dehydrogenase was found to be uncompetitive and reversible. Further, 80mM phosphate inhibited methane monooxygenase and formate dehydrogenase by 16% and 20% respectively. 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| ispartof | The Journal of General and Applied Microbiology, 1987, Vol.33(3), pp.221-229 |
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| source | J-STAGE Free; EZB-FREE-00999 freely available EZB journals |
| subjects | Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Methylosinus trichosporium Microbiology Mission oriented research Physiology and metabolism |
| title | METHANOL ACCUMULATION BY RESTING CELLS OF METHYLOSINUS TRICHOSPORIUM (I) |
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