Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid
Abstract 2,4-Dichlorophenoxyacetic acid is a selective systemic herbicide for the control of broad-leaved weeds, which is widely used throughout the world. The persistence of its residues and its potential to migrate in the soil make it necessary to reduce its concentrations in contaminated soil and...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology ecology 2006-08, Vol.57 (2), p.302-310 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 310 |
|---|---|
| container_issue | 2 |
| container_start_page | 302 |
| container_title | FEMS microbiology ecology |
| container_volume | 57 |
| creator | Germaine, Kieran J. Liu, Xuemei Cabellos, Guiomar Garcia Hogan, Jill P. Ryan, David Dowling, David N. |
| description | Abstract
2,4-Dichlorophenoxyacetic acid is a selective systemic herbicide for the control of broad-leaved weeds, which is widely used throughout the world. The persistence of its residues and its potential to migrate in the soil make it necessary to reduce its concentrations in contaminated soil and groundwater. The nature of this compound makes it particularly toxic to the broad-leaved plants, such as the poplar (Populus) and willow (Salix), which are often used in phytoremediation projects. We describe the inoculation of a model plant, the pea (Pisum sativum), with a genetically tagged bacterial endophyte that naturally possesses the ability to degrade 2,4-dichlorophenoxyacetic acid. The results showed that this strain actively colonized inoculated plants internally (and in the rhizosphere). Inoculated plants showed a higher capacity for 2,4-dichlorophenoxyacetic acid removal from soil and showed no 2,4-dichlorophenoxyacetic acid accumulation in their aerial tissues. This demonstrates the usefulness of bacterial endophytes to enhance the phytoremediation of herbicide-contaminated substrates and reduce levels of toxic herbicide residues in crop plants. |
| doi_str_mv | 10.1111/j.1574-6941.2006.00121.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_14780313</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6941.2006.00121.x</oup_id><sourcerecordid>2307154739</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5361-7d813315c57cb55b79cec01797428f4d420a6cdf7771a2788c33b973d73c5c033</originalsourceid><addsrcrecordid>eNp1kVFv2yAUhdHUaU2z_YUKqVKfZg8MGFvqSxul7aRMe9meEYabmsiBFNtq8u-Hm66VOo0XuJzvXME9CGFKcprWt01OheRZWXOaF4SUOSG0oPn-A5q9CidoRmhZZSWvy1N01vebBAnGySd0mu5LSbmcoe5GmwGi0x0Gb8OuPQyQgW-1N2DxVIYIW7BODy54HNZ4aAGH-KB9MG0XovOAW4iNM84CLr7yzLpnIfUCH_YHbWBwBuukf0Yf17rr4cvLPke_b5e_FvfZ6ufd98X1KjOClTSTtqKMUWGENI0QjawNGEJlLXlRrbnlBdGlsWspJdWFrCrDWFNLZiUzwhDG5ujy2HcXw-MI_aC2rjfQddpDGHuVfl4RRifw4h24CWP06W2qYERSwSWrE3X-Qo1NGoXaRbfV8aD-TjEBV0fgyXVweNOJmtJSGzWFoqZQ1JSWek5L7dXt8kc6JDs72sO4-485-8fM_gBswpbj</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2307154739</pqid></control><display><type>article</type><title>Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Oxford Journals Open Access Collection</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Germaine, Kieran J. ; Liu, Xuemei ; Cabellos, Guiomar Garcia ; Hogan, Jill P. ; Ryan, David ; Dowling, David N.</creator><creatorcontrib>Germaine, Kieran J. ; Liu, Xuemei ; Cabellos, Guiomar Garcia ; Hogan, Jill P. ; Ryan, David ; Dowling, David N.</creatorcontrib><description>Abstract
2,4-Dichlorophenoxyacetic acid is a selective systemic herbicide for the control of broad-leaved weeds, which is widely used throughout the world. The persistence of its residues and its potential to migrate in the soil make it necessary to reduce its concentrations in contaminated soil and groundwater. The nature of this compound makes it particularly toxic to the broad-leaved plants, such as the poplar (Populus) and willow (Salix), which are often used in phytoremediation projects. We describe the inoculation of a model plant, the pea (Pisum sativum), with a genetically tagged bacterial endophyte that naturally possesses the ability to degrade 2,4-dichlorophenoxyacetic acid. The results showed that this strain actively colonized inoculated plants internally (and in the rhizosphere). Inoculated plants showed a higher capacity for 2,4-dichlorophenoxyacetic acid removal from soil and showed no 2,4-dichlorophenoxyacetic acid accumulation in their aerial tissues. This demonstrates the usefulness of bacterial endophytes to enhance the phytoremediation of herbicide-contaminated substrates and reduce levels of toxic herbicide residues in crop plants.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2006.00121.x</identifier><identifier>PMID: 16867147</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>2,4-Dichlorophenoxyacetic Acid - metabolism ; 2,4-Dichlorophenoxyacetic Acid - toxicity ; Acidic soils ; Acids ; Bacteria ; Biodegradation, Environmental ; Biofilms - growth & development ; Biomass ; Chlorophyll - analysis ; Colony Count, Microbial ; Crop residues ; Dichlorophenoxyacetic acid ; Ecology ; endophyte colonization ; Endophytes ; gfp ; Groundwater ; Herbicide residues ; Herbicides ; Inoculation ; Microbiology ; Organic compounds ; Organochlorine compounds ; Phytoremediation ; Pisum sativum ; Pisum sativum - drug effects ; Pisum sativum - metabolism ; Pisum sativum - microbiology ; Plant Roots - microbiology ; Poplar ; Pseudomonas ; Pseudomonas putida - growth & development ; Pseudomonas putida - metabolism ; Residues ; Rhizosphere ; Soil ; Soil contamination ; Soil pollution ; Soils ; Substrates ; Willow</subject><ispartof>FEMS microbiology ecology, 2006-08, Vol.57 (2), p.302-310</ispartof><rights>2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2006</rights><rights>2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5361-7d813315c57cb55b79cec01797428f4d420a6cdf7771a2788c33b973d73c5c033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2006.00121.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2006.00121.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16867147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Germaine, Kieran J.</creatorcontrib><creatorcontrib>Liu, Xuemei</creatorcontrib><creatorcontrib>Cabellos, Guiomar Garcia</creatorcontrib><creatorcontrib>Hogan, Jill P.</creatorcontrib><creatorcontrib>Ryan, David</creatorcontrib><creatorcontrib>Dowling, David N.</creatorcontrib><title>Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
2,4-Dichlorophenoxyacetic acid is a selective systemic herbicide for the control of broad-leaved weeds, which is widely used throughout the world. The persistence of its residues and its potential to migrate in the soil make it necessary to reduce its concentrations in contaminated soil and groundwater. The nature of this compound makes it particularly toxic to the broad-leaved plants, such as the poplar (Populus) and willow (Salix), which are often used in phytoremediation projects. We describe the inoculation of a model plant, the pea (Pisum sativum), with a genetically tagged bacterial endophyte that naturally possesses the ability to degrade 2,4-dichlorophenoxyacetic acid. The results showed that this strain actively colonized inoculated plants internally (and in the rhizosphere). Inoculated plants showed a higher capacity for 2,4-dichlorophenoxyacetic acid removal from soil and showed no 2,4-dichlorophenoxyacetic acid accumulation in their aerial tissues. This demonstrates the usefulness of bacterial endophytes to enhance the phytoremediation of herbicide-contaminated substrates and reduce levels of toxic herbicide residues in crop plants.</description><subject>2,4-Dichlorophenoxyacetic Acid - metabolism</subject><subject>2,4-Dichlorophenoxyacetic Acid - toxicity</subject><subject>Acidic soils</subject><subject>Acids</subject><subject>Bacteria</subject><subject>Biodegradation, Environmental</subject><subject>Biofilms - growth & development</subject><subject>Biomass</subject><subject>Chlorophyll - analysis</subject><subject>Colony Count, Microbial</subject><subject>Crop residues</subject><subject>Dichlorophenoxyacetic acid</subject><subject>Ecology</subject><subject>endophyte colonization</subject><subject>Endophytes</subject><subject>gfp</subject><subject>Groundwater</subject><subject>Herbicide residues</subject><subject>Herbicides</subject><subject>Inoculation</subject><subject>Microbiology</subject><subject>Organic compounds</subject><subject>Organochlorine compounds</subject><subject>Phytoremediation</subject><subject>Pisum sativum</subject><subject>Pisum sativum - drug effects</subject><subject>Pisum sativum - metabolism</subject><subject>Pisum sativum - microbiology</subject><subject>Plant Roots - microbiology</subject><subject>Poplar</subject><subject>Pseudomonas</subject><subject>Pseudomonas putida - growth & development</subject><subject>Pseudomonas putida - metabolism</subject><subject>Residues</subject><subject>Rhizosphere</subject><subject>Soil</subject><subject>Soil contamination</subject><subject>Soil pollution</subject><subject>Soils</subject><subject>Substrates</subject><subject>Willow</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kVFv2yAUhdHUaU2z_YUKqVKfZg8MGFvqSxul7aRMe9meEYabmsiBFNtq8u-Hm66VOo0XuJzvXME9CGFKcprWt01OheRZWXOaF4SUOSG0oPn-A5q9CidoRmhZZSWvy1N01vebBAnGySd0mu5LSbmcoe5GmwGi0x0Gb8OuPQyQgW-1N2DxVIYIW7BODy54HNZ4aAGH-KB9MG0XovOAW4iNM84CLr7yzLpnIfUCH_YHbWBwBuukf0Yf17rr4cvLPke_b5e_FvfZ6ufd98X1KjOClTSTtqKMUWGENI0QjawNGEJlLXlRrbnlBdGlsWspJdWFrCrDWFNLZiUzwhDG5ujy2HcXw-MI_aC2rjfQddpDGHuVfl4RRifw4h24CWP06W2qYERSwSWrE3X-Qo1NGoXaRbfV8aD-TjEBV0fgyXVweNOJmtJSGzWFoqZQ1JSWek5L7dXt8kc6JDs72sO4-485-8fM_gBswpbj</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Germaine, Kieran J.</creator><creator>Liu, Xuemei</creator><creator>Cabellos, Guiomar Garcia</creator><creator>Hogan, Jill P.</creator><creator>Ryan, David</creator><creator>Dowling, David N.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7ST</scope><scope>SOI</scope></search><sort><creationdate>20060801</creationdate><title>Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid</title><author>Germaine, Kieran J. ; Liu, Xuemei ; Cabellos, Guiomar Garcia ; Hogan, Jill P. ; Ryan, David ; Dowling, David N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5361-7d813315c57cb55b79cec01797428f4d420a6cdf7771a2788c33b973d73c5c033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>2,4-Dichlorophenoxyacetic Acid - metabolism</topic><topic>2,4-Dichlorophenoxyacetic Acid - toxicity</topic><topic>Acidic soils</topic><topic>Acids</topic><topic>Bacteria</topic><topic>Biodegradation, Environmental</topic><topic>Biofilms - growth & development</topic><topic>Biomass</topic><topic>Chlorophyll - analysis</topic><topic>Colony Count, Microbial</topic><topic>Crop residues</topic><topic>Dichlorophenoxyacetic acid</topic><topic>Ecology</topic><topic>endophyte colonization</topic><topic>Endophytes</topic><topic>gfp</topic><topic>Groundwater</topic><topic>Herbicide residues</topic><topic>Herbicides</topic><topic>Inoculation</topic><topic>Microbiology</topic><topic>Organic compounds</topic><topic>Organochlorine compounds</topic><topic>Phytoremediation</topic><topic>Pisum sativum</topic><topic>Pisum sativum - drug effects</topic><topic>Pisum sativum - metabolism</topic><topic>Pisum sativum - microbiology</topic><topic>Plant Roots - microbiology</topic><topic>Poplar</topic><topic>Pseudomonas</topic><topic>Pseudomonas putida - growth & development</topic><topic>Pseudomonas putida - metabolism</topic><topic>Residues</topic><topic>Rhizosphere</topic><topic>Soil</topic><topic>Soil contamination</topic><topic>Soil pollution</topic><topic>Soils</topic><topic>Substrates</topic><topic>Willow</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Germaine, Kieran J.</creatorcontrib><creatorcontrib>Liu, Xuemei</creatorcontrib><creatorcontrib>Cabellos, Guiomar Garcia</creatorcontrib><creatorcontrib>Hogan, Jill P.</creatorcontrib><creatorcontrib>Ryan, David</creatorcontrib><creatorcontrib>Dowling, David N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Germaine, Kieran J.</au><au>Liu, Xuemei</au><au>Cabellos, Guiomar Garcia</au><au>Hogan, Jill P.</au><au>Ryan, David</au><au>Dowling, David N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>57</volume><issue>2</issue><spage>302</spage><epage>310</epage><pages>302-310</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Abstract
2,4-Dichlorophenoxyacetic acid is a selective systemic herbicide for the control of broad-leaved weeds, which is widely used throughout the world. The persistence of its residues and its potential to migrate in the soil make it necessary to reduce its concentrations in contaminated soil and groundwater. The nature of this compound makes it particularly toxic to the broad-leaved plants, such as the poplar (Populus) and willow (Salix), which are often used in phytoremediation projects. We describe the inoculation of a model plant, the pea (Pisum sativum), with a genetically tagged bacterial endophyte that naturally possesses the ability to degrade 2,4-dichlorophenoxyacetic acid. The results showed that this strain actively colonized inoculated plants internally (and in the rhizosphere). Inoculated plants showed a higher capacity for 2,4-dichlorophenoxyacetic acid removal from soil and showed no 2,4-dichlorophenoxyacetic acid accumulation in their aerial tissues. This demonstrates the usefulness of bacterial endophytes to enhance the phytoremediation of herbicide-contaminated substrates and reduce levels of toxic herbicide residues in crop plants.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16867147</pmid><doi>10.1111/j.1574-6941.2006.00121.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-6496 |
| ispartof | FEMS microbiology ecology, 2006-08, Vol.57 (2), p.302-310 |
| issn | 0168-6496 1574-6941 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14780313 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford Journals Open Access Collection; EZB-FREE-00999 freely available EZB journals |
| subjects | 2,4-Dichlorophenoxyacetic Acid - metabolism 2,4-Dichlorophenoxyacetic Acid - toxicity Acidic soils Acids Bacteria Biodegradation, Environmental Biofilms - growth & development Biomass Chlorophyll - analysis Colony Count, Microbial Crop residues Dichlorophenoxyacetic acid Ecology endophyte colonization Endophytes gfp Groundwater Herbicide residues Herbicides Inoculation Microbiology Organic compounds Organochlorine compounds Phytoremediation Pisum sativum Pisum sativum - drug effects Pisum sativum - metabolism Pisum sativum - microbiology Plant Roots - microbiology Poplar Pseudomonas Pseudomonas putida - growth & development Pseudomonas putida - metabolism Residues Rhizosphere Soil Soil contamination Soil pollution Soils Substrates Willow |
| title | Bacterial endophyte-enhanced phytoremediation of the organochlorine herbicide 2,4-dichlorophenoxyacetic acid |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T18%3A36%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20endophyte-enhanced%20phytoremediation%20of%20the%20organochlorine%20herbicide%202,4-dichlorophenoxyacetic%20acid&rft.jtitle=FEMS%20microbiology%20ecology&rft.au=Germaine,%20Kieran%20J.&rft.date=2006-08-01&rft.volume=57&rft.issue=2&rft.spage=302&rft.epage=310&rft.pages=302-310&rft.issn=0168-6496&rft.eissn=1574-6941&rft_id=info:doi/10.1111/j.1574-6941.2006.00121.x&rft_dat=%3Cproquest_pubme%3E2307154739%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2307154739&rft_id=info:pmid/16867147&rft_oup_id=10.1111/j.1574-6941.2006.00121.x&rfr_iscdi=true |