Effects of Tacrolimus and Nifedipine, Alone or in Combination, on Gingival Tissues
Background: The aim of this study is to compare gingival changes induced by short‐ and long‐term tacrolimus and nifedipine administration, alone or in combination, and evaluate the expression levels of tumor suppressor phosphatase and tensin homolog (PTEN) in drug‐induced gingival overgrowth. Method...
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| Veröffentlicht in: | Journal of periodontology (1970) 2013-11, Vol.84 (11), p.1673-1682 |
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| container_title | Journal of periodontology (1970) |
| container_volume | 84 |
| creator | Pamuk, Ferda Cetinkaya, Burcu Ozkan Gulbahar, Mustafa Yavuz Gacar, Ayhan Cayır Keles, Gonca Erisgin, Zuleyha Arik, Nurol |
| description | Background: The aim of this study is to compare gingival changes induced by short‐ and long‐term tacrolimus and nifedipine administration, alone or in combination, and evaluate the expression levels of tumor suppressor phosphatase and tensin homolog (PTEN) in drug‐induced gingival overgrowth.
Methods: Eighty rats were equally divided into eight groups: 1) tacrolimus for 8 weeks; 2) nifedipine for 8 weeks; 3) tacrolimus and nifedipine for 8 weeks; 4) 8‐week control; 5) tacrolimus for 24 weeks; 6) nifedipine for 24 weeks; 7) tacrolimus and nifedipine for 24 weeks; and 8) 24‐week control. Histomorphometric analyses included measurements of epithelial thickness, connective tissue thickness, and height. Stereologic analyses included measurements of volumetric densities of fibroblasts (Vf), collagen fibers (Vcf), and blood vessels (Vbv). In addition, PTEN expression was analyzed using immunohistochemistry.
Results: Epithelial thickness and connective tissue thickness were significantly increased in groups 5, 6, and 7 compared to group 8 (P |
| doi_str_mv | 10.1902/jop.2013.120545 |
| format | Article |
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Methods: Eighty rats were equally divided into eight groups: 1) tacrolimus for 8 weeks; 2) nifedipine for 8 weeks; 3) tacrolimus and nifedipine for 8 weeks; 4) 8‐week control; 5) tacrolimus for 24 weeks; 6) nifedipine for 24 weeks; 7) tacrolimus and nifedipine for 24 weeks; and 8) 24‐week control. Histomorphometric analyses included measurements of epithelial thickness, connective tissue thickness, and height. Stereologic analyses included measurements of volumetric densities of fibroblasts (Vf), collagen fibers (Vcf), and blood vessels (Vbv). In addition, PTEN expression was analyzed using immunohistochemistry.
Results: Epithelial thickness and connective tissue thickness were significantly increased in groups 5, 6, and 7 compared to group 8 (P <0.05), whereas connective tissue height was significantly increased in groups 5 and 7 (P <0.001). Vf and Vcf were significantly increased in group 7 compared to group 8 (P <0.001). PTEN immunoreactivity was significantly decreased in all experimental groups compared to the control groups (P <0.05).
Conclusions: Results suggest that duration of drug administration is a more important risk factor than drug combination. The results include a potentially new insight about PTEN's role in the etiology of drug‐induced gingival overgrowth.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1902/jop.2013.120545</identifier><identifier>PMID: 23289868</identifier><language>eng</language><publisher>United States: American Academy of Periodontology</publisher><subject>Animals ; Blood Vessels - drug effects ; Calcium Channel Blockers - administration & dosage ; Calcium Channel Blockers - adverse effects ; Calcium Channel Blockers - pharmacology ; Cell Count ; Collagen - drug effects ; Connective Tissue - drug effects ; Connective Tissue - pathology ; Dentistry ; Drug Combinations ; Epithelium - drug effects ; Epithelium - pathology ; Fibroblasts - drug effects ; Fibroblasts - pathology ; Gene Expression Regulation, Enzymologic - drug effects ; Gingiva - drug effects ; Gingiva - pathology ; Gingival overgrowth ; Gingival Overgrowth - chemically induced ; immunohistochemistry ; Immunosuppressive Agents - administration & dosage ; Immunosuppressive Agents - adverse effects ; Immunosuppressive Agents - pharmacology ; Male ; nifedipine ; Nifedipine - administration & dosage ; Nifedipine - adverse effects ; Nifedipine - pharmacology ; PTEN Phosphohydrolase - drug effects ; PTEN Phosphohydrolase - genetics ; PTEN protein ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; tacrolimus ; Tacrolimus - administration & dosage ; Tacrolimus - adverse effects ; Tacrolimus - pharmacology ; Time Factors</subject><ispartof>Journal of periodontology (1970), 2013-11, Vol.84 (11), p.1673-1682</ispartof><rights>2013 American Academy of Periodontology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3433-f9ff7bc4b377993dbc98c8720fd3e63bc89e0f2c74fa0bed6cf04add966bb6a83</citedby><cites>FETCH-LOGICAL-c3433-f9ff7bc4b377993dbc98c8720fd3e63bc89e0f2c74fa0bed6cf04add966bb6a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1902%2Fjop.2013.120545$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1902%2Fjop.2013.120545$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23289868$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pamuk, Ferda</creatorcontrib><creatorcontrib>Cetinkaya, Burcu Ozkan</creatorcontrib><creatorcontrib>Gulbahar, Mustafa Yavuz</creatorcontrib><creatorcontrib>Gacar, Ayhan</creatorcontrib><creatorcontrib>Cayır Keles, Gonca</creatorcontrib><creatorcontrib>Erisgin, Zuleyha</creatorcontrib><creatorcontrib>Arik, Nurol</creatorcontrib><title>Effects of Tacrolimus and Nifedipine, Alone or in Combination, on Gingival Tissues</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background: The aim of this study is to compare gingival changes induced by short‐ and long‐term tacrolimus and nifedipine administration, alone or in combination, and evaluate the expression levels of tumor suppressor phosphatase and tensin homolog (PTEN) in drug‐induced gingival overgrowth.
Methods: Eighty rats were equally divided into eight groups: 1) tacrolimus for 8 weeks; 2) nifedipine for 8 weeks; 3) tacrolimus and nifedipine for 8 weeks; 4) 8‐week control; 5) tacrolimus for 24 weeks; 6) nifedipine for 24 weeks; 7) tacrolimus and nifedipine for 24 weeks; and 8) 24‐week control. Histomorphometric analyses included measurements of epithelial thickness, connective tissue thickness, and height. Stereologic analyses included measurements of volumetric densities of fibroblasts (Vf), collagen fibers (Vcf), and blood vessels (Vbv). In addition, PTEN expression was analyzed using immunohistochemistry.
Results: Epithelial thickness and connective tissue thickness were significantly increased in groups 5, 6, and 7 compared to group 8 (P <0.05), whereas connective tissue height was significantly increased in groups 5 and 7 (P <0.001). Vf and Vcf were significantly increased in group 7 compared to group 8 (P <0.001). PTEN immunoreactivity was significantly decreased in all experimental groups compared to the control groups (P <0.05).
Conclusions: Results suggest that duration of drug administration is a more important risk factor than drug combination. The results include a potentially new insight about PTEN's role in the etiology of drug‐induced gingival overgrowth.</description><subject>Animals</subject><subject>Blood Vessels - drug effects</subject><subject>Calcium Channel Blockers - administration & dosage</subject><subject>Calcium Channel Blockers - adverse effects</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Cell Count</subject><subject>Collagen - drug effects</subject><subject>Connective Tissue - drug effects</subject><subject>Connective Tissue - pathology</subject><subject>Dentistry</subject><subject>Drug Combinations</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - pathology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - pathology</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gingiva - drug effects</subject><subject>Gingiva - pathology</subject><subject>Gingival overgrowth</subject><subject>Gingival Overgrowth - chemically induced</subject><subject>immunohistochemistry</subject><subject>Immunosuppressive Agents - administration & dosage</subject><subject>Immunosuppressive Agents - adverse effects</subject><subject>Immunosuppressive Agents - pharmacology</subject><subject>Male</subject><subject>nifedipine</subject><subject>Nifedipine - administration & dosage</subject><subject>Nifedipine - adverse effects</subject><subject>Nifedipine - pharmacology</subject><subject>PTEN Phosphohydrolase - drug effects</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN protein</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>tacrolimus</subject><subject>Tacrolimus - administration & dosage</subject><subject>Tacrolimus - adverse effects</subject><subject>Tacrolimus - pharmacology</subject><subject>Time Factors</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEURYMoWqtrd5KlC6fN1ySTpZRalaIidR2STCKRmaROOor_3pFRt64eD869j3cAOMNohiUi89e0nRGE6QwTVLJyD0ywZLSgXKB9MEGIkIIySY7Acc6vw4oZRYfgiFBSyYpXE_C09N7ZXYbJw422XWpC22eoYw3vg3d12IboLuFVk6KDqYMhwkVqTYh6F1K8hCnCVYgv4V03cBNy7l0-AQdeN9md_swpeL5ebhY3xfphdbu4WheWMkoLL70XxjJDhZCS1sbKylaCIF9Tx6mxlXTIEyuY18i4mluPmK5rybkxXFd0Ci7G3m2X3oa7O9WGbF3T6OhSnxVmQpQci5IM6HxEhwdz7pxX2y60uvtUGKlvkWoQqb5FqlHkkDj_Ke9N6-o__tfcAJQj8BEa9_lfn7p7XD5hLij9AjLTf0E</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Pamuk, Ferda</creator><creator>Cetinkaya, Burcu Ozkan</creator><creator>Gulbahar, Mustafa Yavuz</creator><creator>Gacar, Ayhan</creator><creator>Cayır Keles, Gonca</creator><creator>Erisgin, Zuleyha</creator><creator>Arik, Nurol</creator><general>American Academy of Periodontology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201311</creationdate><title>Effects of Tacrolimus and Nifedipine, Alone or in Combination, on Gingival Tissues</title><author>Pamuk, Ferda ; Cetinkaya, Burcu Ozkan ; Gulbahar, Mustafa Yavuz ; Gacar, Ayhan ; Cayır Keles, Gonca ; Erisgin, Zuleyha ; Arik, Nurol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3433-f9ff7bc4b377993dbc98c8720fd3e63bc89e0f2c74fa0bed6cf04add966bb6a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Blood Vessels - drug effects</topic><topic>Calcium Channel Blockers - administration & dosage</topic><topic>Calcium Channel Blockers - adverse effects</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Cell Count</topic><topic>Collagen - drug effects</topic><topic>Connective Tissue - drug effects</topic><topic>Connective Tissue - pathology</topic><topic>Dentistry</topic><topic>Drug Combinations</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - pathology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - pathology</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gingiva - drug effects</topic><topic>Gingiva - pathology</topic><topic>Gingival overgrowth</topic><topic>Gingival Overgrowth - chemically induced</topic><topic>immunohistochemistry</topic><topic>Immunosuppressive Agents - administration & dosage</topic><topic>Immunosuppressive Agents - adverse effects</topic><topic>Immunosuppressive Agents - pharmacology</topic><topic>Male</topic><topic>nifedipine</topic><topic>Nifedipine - administration & dosage</topic><topic>Nifedipine - adverse effects</topic><topic>Nifedipine - pharmacology</topic><topic>PTEN Phosphohydrolase - drug effects</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>PTEN protein</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>tacrolimus</topic><topic>Tacrolimus - administration & dosage</topic><topic>Tacrolimus - adverse effects</topic><topic>Tacrolimus - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pamuk, Ferda</creatorcontrib><creatorcontrib>Cetinkaya, Burcu Ozkan</creatorcontrib><creatorcontrib>Gulbahar, Mustafa Yavuz</creatorcontrib><creatorcontrib>Gacar, Ayhan</creatorcontrib><creatorcontrib>Cayır Keles, Gonca</creatorcontrib><creatorcontrib>Erisgin, Zuleyha</creatorcontrib><creatorcontrib>Arik, Nurol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pamuk, Ferda</au><au>Cetinkaya, Burcu Ozkan</au><au>Gulbahar, Mustafa Yavuz</au><au>Gacar, Ayhan</au><au>Cayır Keles, Gonca</au><au>Erisgin, Zuleyha</au><au>Arik, Nurol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Tacrolimus and Nifedipine, Alone or in Combination, on Gingival Tissues</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2013-11</date><risdate>2013</risdate><volume>84</volume><issue>11</issue><spage>1673</spage><epage>1682</epage><pages>1673-1682</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background: The aim of this study is to compare gingival changes induced by short‐ and long‐term tacrolimus and nifedipine administration, alone or in combination, and evaluate the expression levels of tumor suppressor phosphatase and tensin homolog (PTEN) in drug‐induced gingival overgrowth.
Methods: Eighty rats were equally divided into eight groups: 1) tacrolimus for 8 weeks; 2) nifedipine for 8 weeks; 3) tacrolimus and nifedipine for 8 weeks; 4) 8‐week control; 5) tacrolimus for 24 weeks; 6) nifedipine for 24 weeks; 7) tacrolimus and nifedipine for 24 weeks; and 8) 24‐week control. Histomorphometric analyses included measurements of epithelial thickness, connective tissue thickness, and height. Stereologic analyses included measurements of volumetric densities of fibroblasts (Vf), collagen fibers (Vcf), and blood vessels (Vbv). In addition, PTEN expression was analyzed using immunohistochemistry.
Results: Epithelial thickness and connective tissue thickness were significantly increased in groups 5, 6, and 7 compared to group 8 (P <0.05), whereas connective tissue height was significantly increased in groups 5 and 7 (P <0.001). Vf and Vcf were significantly increased in group 7 compared to group 8 (P <0.001). PTEN immunoreactivity was significantly decreased in all experimental groups compared to the control groups (P <0.05).
Conclusions: Results suggest that duration of drug administration is a more important risk factor than drug combination. The results include a potentially new insight about PTEN's role in the etiology of drug‐induced gingival overgrowth.</abstract><cop>United States</cop><pub>American Academy of Periodontology</pub><pmid>23289868</pmid><doi>10.1902/jop.2013.120545</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0022-3492 |
| ispartof | Journal of periodontology (1970), 2013-11, Vol.84 (11), p.1673-1682 |
| issn | 0022-3492 1943-3670 |
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| subjects | Animals Blood Vessels - drug effects Calcium Channel Blockers - administration & dosage Calcium Channel Blockers - adverse effects Calcium Channel Blockers - pharmacology Cell Count Collagen - drug effects Connective Tissue - drug effects Connective Tissue - pathology Dentistry Drug Combinations Epithelium - drug effects Epithelium - pathology Fibroblasts - drug effects Fibroblasts - pathology Gene Expression Regulation, Enzymologic - drug effects Gingiva - drug effects Gingiva - pathology Gingival overgrowth Gingival Overgrowth - chemically induced immunohistochemistry Immunosuppressive Agents - administration & dosage Immunosuppressive Agents - adverse effects Immunosuppressive Agents - pharmacology Male nifedipine Nifedipine - administration & dosage Nifedipine - adverse effects Nifedipine - pharmacology PTEN Phosphohydrolase - drug effects PTEN Phosphohydrolase - genetics PTEN protein Random Allocation Rats Rats, Sprague-Dawley tacrolimus Tacrolimus - administration & dosage Tacrolimus - adverse effects Tacrolimus - pharmacology Time Factors |
| title | Effects of Tacrolimus and Nifedipine, Alone or in Combination, on Gingival Tissues |
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