A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes
An impact on glycation, and possibly on diabetic complications, is attributed to fructosamine-3-kinase (FN3K) and its related protein (FN3K-RP) because they degrade Amadori compounds in vivo. Little is known about individual differences in FN3K-RP activity, which might contribute to an individual ri...
Gespeichert in:
| Veröffentlicht in: | Clinical chemistry and laboratory medicine 2014-01, Vol.52 (1), p.93-101 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 101 |
|---|---|
| container_issue | 1 |
| container_start_page | 93 |
| container_title | Clinical chemistry and laboratory medicine |
| container_volume | 52 |
| creator | Hellwig, Anne Scherber, Anja Koehler, Carsta Hanefeld, Markolf Henle, Thomas |
| description | An impact on glycation, and possibly on diabetic complications, is attributed to fructosamine-3-kinase (FN3K) and its related protein (FN3K-RP) because they degrade Amadori compounds in vivo. Little is known about individual differences in FN3K-RP activity, which might contribute to an individual risk for diabetic complications.
An HPLC-based activity assay for FN3K-RP in erythrocytes with the substrate
-α-hippuryl-
-ε-psicosyllysine was developed. The activities of FN3K and FN3K-RP were also analysed in erythrocytes of 103 consecutive participants of a health-care survey amongst a high-risk group for diabetes. The potential associations of these activities with the subjects’ health background (anthropometric data, glucose tolerance and HbA
, blood lipids, history of metabolic diseases in the subjects and their families, and medication) were examined.
The interindividual variability of FN3K-RP is less pronounced than that of FN3K [60–135 vs. 2.8–12.5 mU/g haemoglobin (Hb)]. No correlations with age, sex, body weight, blood cholesterol, or plasma glucose in an oral glucose tolerance test were observed. Subjects with kidney disease had higher activity of mainly FN3K-RP [111±15 vs. 98±18 mU/g Hb, mean±standard deviations (SDs), n=16 vs. 87, p=0.009], whereas subjects whose parents or siblings had a stroke showed lower FN3K activity (6.2±1.6 vs. 7.1±1.8 mU/g Hb, mean±SD, n=24 vs. 66, p=0.040).
There is a likely impact of FN3K and FN3K-RP on the glycation cascade in vivo with potential positive and negative effects. The new screening method enables further studies to elucidate the function and importance of FN3K-RP. |
| doi_str_mv | 10.1515/cclm-2012-0853 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1477560461</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1477560461</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-adeeae95ee7d1b6cf8f977834cc2871aae255cb8024bafcca039f48c887e18e73</originalsourceid><addsrcrecordid>eNp1kD1vFDEQhi0EIiHQUiKXoXDw59onquiUEMSJpIDamvWOuQ37EWwv0bb8cna5kC7VvMUzr2YeQt4KfiaMMB9C6HomuZCMO6OekWOhlWVaKfH8X9asqqQ4Iq9yvuVcGKPtS3IkVaVdpdQx-XNOB7ynVze7LashY0MhZ5hpHBMte6Q9Qp4S9jgUOkYa0xTKmKFvB2SK_WyHZYeeXn5VX95TGBq6Jpawg7JU3aWxYDtQCKX93ZaZLnk_9TBQTHPZpzHMBfNr8iJCl_HNwzwh3y8vvm2v2O760-ft-Y4FzWVh0CACbgyibURdhejixlqndAjSWQGA0phQOy51DTEE4GoTtQvOWRQOrTohp4fe5axfE-bi-zYH7DoYcJyyF9paU3FdiQU9O6AhjTknjP4utT2k2QvuV-9-9e5X7371viy8e-ie6h6bR_y_6AX4eADuoSuYGvyRpuX55G_HKQ3L2080Gyk2Sv0FqpOTQQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1477560461</pqid></control><display><type>article</type><title>A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes</title><source>MEDLINE</source><source>De Gruyter journals</source><creator>Hellwig, Anne ; Scherber, Anja ; Koehler, Carsta ; Hanefeld, Markolf ; Henle, Thomas</creator><creatorcontrib>Hellwig, Anne ; Scherber, Anja ; Koehler, Carsta ; Hanefeld, Markolf ; Henle, Thomas</creatorcontrib><description>An impact on glycation, and possibly on diabetic complications, is attributed to fructosamine-3-kinase (FN3K) and its related protein (FN3K-RP) because they degrade Amadori compounds in vivo. Little is known about individual differences in FN3K-RP activity, which might contribute to an individual risk for diabetic complications.
An HPLC-based activity assay for FN3K-RP in erythrocytes with the substrate
-α-hippuryl-
-ε-psicosyllysine was developed. The activities of FN3K and FN3K-RP were also analysed in erythrocytes of 103 consecutive participants of a health-care survey amongst a high-risk group for diabetes. The potential associations of these activities with the subjects’ health background (anthropometric data, glucose tolerance and HbA
, blood lipids, history of metabolic diseases in the subjects and their families, and medication) were examined.
The interindividual variability of FN3K-RP is less pronounced than that of FN3K [60–135 vs. 2.8–12.5 mU/g haemoglobin (Hb)]. No correlations with age, sex, body weight, blood cholesterol, or plasma glucose in an oral glucose tolerance test were observed. Subjects with kidney disease had higher activity of mainly FN3K-RP [111±15 vs. 98±18 mU/g Hb, mean±standard deviations (SDs), n=16 vs. 87, p=0.009], whereas subjects whose parents or siblings had a stroke showed lower FN3K activity (6.2±1.6 vs. 7.1±1.8 mU/g Hb, mean±SD, n=24 vs. 66, p=0.040).
There is a likely impact of FN3K and FN3K-RP on the glycation cascade in vivo with potential positive and negative effects. The new screening method enables further studies to elucidate the function and importance of FN3K-RP.</description><identifier>ISSN: 1434-6621</identifier><identifier>EISSN: 1437-4331</identifier><identifier>DOI: 10.1515/cclm-2012-0853</identifier><identifier>PMID: 23648633</identifier><language>eng</language><publisher>Germany: De Gruyter</publisher><subject>Aged ; Chromatography, High Pressure Liquid ; diabetic complications ; Erythrocytes - enzymology ; Erythrocytes - metabolism ; Female ; fructosamine-3-kinase (FN3K) ; fructosamine-3-kinase-related protein (FN3K-RP) ; glycation ; Hexoses - analysis ; Hexoses - chemical synthesis ; Hexoses - metabolism ; Humans ; Lysine - analogs & derivatives ; Lysine - analysis ; Lysine - chemical synthesis ; Lysine - metabolism ; Male ; Middle Aged ; Phosphotransferases (Alcohol Group Acceptor) - metabolism ; Substrate Specificity</subject><ispartof>Clinical chemistry and laboratory medicine, 2014-01, Vol.52 (1), p.93-101</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-adeeae95ee7d1b6cf8f977834cc2871aae255cb8024bafcca039f48c887e18e73</citedby><cites>FETCH-LOGICAL-c402t-adeeae95ee7d1b6cf8f977834cc2871aae255cb8024bafcca039f48c887e18e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.degruyter.com/document/doi/10.1515/cclm-2012-0853/pdf$$EPDF$$P50$$Gwalterdegruyter$$H</linktopdf><linktohtml>$$Uhttps://www.degruyter.com/document/doi/10.1515/cclm-2012-0853/html$$EHTML$$P50$$Gwalterdegruyter$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,66503,68287</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23648633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hellwig, Anne</creatorcontrib><creatorcontrib>Scherber, Anja</creatorcontrib><creatorcontrib>Koehler, Carsta</creatorcontrib><creatorcontrib>Hanefeld, Markolf</creatorcontrib><creatorcontrib>Henle, Thomas</creatorcontrib><title>A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes</title><title>Clinical chemistry and laboratory medicine</title><addtitle>Clin Chem Lab Med</addtitle><description>An impact on glycation, and possibly on diabetic complications, is attributed to fructosamine-3-kinase (FN3K) and its related protein (FN3K-RP) because they degrade Amadori compounds in vivo. Little is known about individual differences in FN3K-RP activity, which might contribute to an individual risk for diabetic complications.
An HPLC-based activity assay for FN3K-RP in erythrocytes with the substrate
-α-hippuryl-
-ε-psicosyllysine was developed. The activities of FN3K and FN3K-RP were also analysed in erythrocytes of 103 consecutive participants of a health-care survey amongst a high-risk group for diabetes. The potential associations of these activities with the subjects’ health background (anthropometric data, glucose tolerance and HbA
, blood lipids, history of metabolic diseases in the subjects and their families, and medication) were examined.
The interindividual variability of FN3K-RP is less pronounced than that of FN3K [60–135 vs. 2.8–12.5 mU/g haemoglobin (Hb)]. No correlations with age, sex, body weight, blood cholesterol, or plasma glucose in an oral glucose tolerance test were observed. Subjects with kidney disease had higher activity of mainly FN3K-RP [111±15 vs. 98±18 mU/g Hb, mean±standard deviations (SDs), n=16 vs. 87, p=0.009], whereas subjects whose parents or siblings had a stroke showed lower FN3K activity (6.2±1.6 vs. 7.1±1.8 mU/g Hb, mean±SD, n=24 vs. 66, p=0.040).
There is a likely impact of FN3K and FN3K-RP on the glycation cascade in vivo with potential positive and negative effects. The new screening method enables further studies to elucidate the function and importance of FN3K-RP.</description><subject>Aged</subject><subject>Chromatography, High Pressure Liquid</subject><subject>diabetic complications</subject><subject>Erythrocytes - enzymology</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>fructosamine-3-kinase (FN3K)</subject><subject>fructosamine-3-kinase-related protein (FN3K-RP)</subject><subject>glycation</subject><subject>Hexoses - analysis</subject><subject>Hexoses - chemical synthesis</subject><subject>Hexoses - metabolism</subject><subject>Humans</subject><subject>Lysine - analogs & derivatives</subject><subject>Lysine - analysis</subject><subject>Lysine - chemical synthesis</subject><subject>Lysine - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Phosphotransferases (Alcohol Group Acceptor) - metabolism</subject><subject>Substrate Specificity</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1vFDEQhi0EIiHQUiKXoXDw59onquiUEMSJpIDamvWOuQ37EWwv0bb8cna5kC7VvMUzr2YeQt4KfiaMMB9C6HomuZCMO6OekWOhlWVaKfH8X9asqqQ4Iq9yvuVcGKPtS3IkVaVdpdQx-XNOB7ynVze7LashY0MhZ5hpHBMte6Q9Qp4S9jgUOkYa0xTKmKFvB2SK_WyHZYeeXn5VX95TGBq6Jpawg7JU3aWxYDtQCKX93ZaZLnk_9TBQTHPZpzHMBfNr8iJCl_HNwzwh3y8vvm2v2O760-ft-Y4FzWVh0CACbgyibURdhejixlqndAjSWQGA0phQOy51DTEE4GoTtQvOWRQOrTohp4fe5axfE-bi-zYH7DoYcJyyF9paU3FdiQU9O6AhjTknjP4utT2k2QvuV-9-9e5X7371viy8e-ie6h6bR_y_6AX4eADuoSuYGvyRpuX55G_HKQ3L2080Gyk2Sv0FqpOTQQ</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Hellwig, Anne</creator><creator>Scherber, Anja</creator><creator>Koehler, Carsta</creator><creator>Hanefeld, Markolf</creator><creator>Henle, Thomas</creator><general>De Gruyter</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140101</creationdate><title>A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes</title><author>Hellwig, Anne ; Scherber, Anja ; Koehler, Carsta ; Hanefeld, Markolf ; Henle, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-adeeae95ee7d1b6cf8f977834cc2871aae255cb8024bafcca039f48c887e18e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Chromatography, High Pressure Liquid</topic><topic>diabetic complications</topic><topic>Erythrocytes - enzymology</topic><topic>Erythrocytes - metabolism</topic><topic>Female</topic><topic>fructosamine-3-kinase (FN3K)</topic><topic>fructosamine-3-kinase-related protein (FN3K-RP)</topic><topic>glycation</topic><topic>Hexoses - analysis</topic><topic>Hexoses - chemical synthesis</topic><topic>Hexoses - metabolism</topic><topic>Humans</topic><topic>Lysine - analogs & derivatives</topic><topic>Lysine - analysis</topic><topic>Lysine - chemical synthesis</topic><topic>Lysine - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Phosphotransferases (Alcohol Group Acceptor) - metabolism</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hellwig, Anne</creatorcontrib><creatorcontrib>Scherber, Anja</creatorcontrib><creatorcontrib>Koehler, Carsta</creatorcontrib><creatorcontrib>Hanefeld, Markolf</creatorcontrib><creatorcontrib>Henle, Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry and laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hellwig, Anne</au><au>Scherber, Anja</au><au>Koehler, Carsta</au><au>Hanefeld, Markolf</au><au>Henle, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes</atitle><jtitle>Clinical chemistry and laboratory medicine</jtitle><addtitle>Clin Chem Lab Med</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>52</volume><issue>1</issue><spage>93</spage><epage>101</epage><pages>93-101</pages><issn>1434-6621</issn><eissn>1437-4331</eissn><abstract>An impact on glycation, and possibly on diabetic complications, is attributed to fructosamine-3-kinase (FN3K) and its related protein (FN3K-RP) because they degrade Amadori compounds in vivo. Little is known about individual differences in FN3K-RP activity, which might contribute to an individual risk for diabetic complications.
An HPLC-based activity assay for FN3K-RP in erythrocytes with the substrate
-α-hippuryl-
-ε-psicosyllysine was developed. The activities of FN3K and FN3K-RP were also analysed in erythrocytes of 103 consecutive participants of a health-care survey amongst a high-risk group for diabetes. The potential associations of these activities with the subjects’ health background (anthropometric data, glucose tolerance and HbA
, blood lipids, history of metabolic diseases in the subjects and their families, and medication) were examined.
The interindividual variability of FN3K-RP is less pronounced than that of FN3K [60–135 vs. 2.8–12.5 mU/g haemoglobin (Hb)]. No correlations with age, sex, body weight, blood cholesterol, or plasma glucose in an oral glucose tolerance test were observed. Subjects with kidney disease had higher activity of mainly FN3K-RP [111±15 vs. 98±18 mU/g Hb, mean±standard deviations (SDs), n=16 vs. 87, p=0.009], whereas subjects whose parents or siblings had a stroke showed lower FN3K activity (6.2±1.6 vs. 7.1±1.8 mU/g Hb, mean±SD, n=24 vs. 66, p=0.040).
There is a likely impact of FN3K and FN3K-RP on the glycation cascade in vivo with potential positive and negative effects. The new screening method enables further studies to elucidate the function and importance of FN3K-RP.</abstract><cop>Germany</cop><pub>De Gruyter</pub><pmid>23648633</pmid><doi>10.1515/cclm-2012-0853</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1434-6621 |
| ispartof | Clinical chemistry and laboratory medicine, 2014-01, Vol.52 (1), p.93-101 |
| issn | 1434-6621 1437-4331 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1477560461 |
| source | MEDLINE; De Gruyter journals |
| subjects | Aged Chromatography, High Pressure Liquid diabetic complications Erythrocytes - enzymology Erythrocytes - metabolism Female fructosamine-3-kinase (FN3K) fructosamine-3-kinase-related protein (FN3K-RP) glycation Hexoses - analysis Hexoses - chemical synthesis Hexoses - metabolism Humans Lysine - analogs & derivatives Lysine - analysis Lysine - chemical synthesis Lysine - metabolism Male Middle Aged Phosphotransferases (Alcohol Group Acceptor) - metabolism Substrate Specificity |
| title | A new HPLC-based assay for the measurement of fructosamine-3-kinase (FN3K) and FN3K-related protein activity in human erythrocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T17%3A05%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20new%20HPLC-based%20assay%20for%20the%20measurement%20of%20fructosamine-3-kinase%20(FN3K)%20and%20FN3K-related%20protein%20activity%20in%20human%20erythrocytes&rft.jtitle=Clinical%20chemistry%20and%20laboratory%20medicine&rft.au=Hellwig,%20Anne&rft.date=2014-01-01&rft.volume=52&rft.issue=1&rft.spage=93&rft.epage=101&rft.pages=93-101&rft.issn=1434-6621&rft.eissn=1437-4331&rft_id=info:doi/10.1515/cclm-2012-0853&rft_dat=%3Cproquest_cross%3E1477560461%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1477560461&rft_id=info:pmid/23648633&rfr_iscdi=true |