Production of fructosyl transferase from Aureobasidium pullulans
Conditions for the production of intracellular fructosyl transferase from A. pullulans were investigated. Sucrose was an excellent carbon source, and there was a tendency for the enzyme production to be increased as sucrose concentration was increased. Both 0.5% phosphate and 2% sodium nitrate had p...
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Veröffentlicht in: | Biotechnology letters 1987-01, Vol.9 (10), p.703-708 |
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container_title | Biotechnology letters |
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creator | Jung, K.H Lim, J.Y Yoo, S.J Lee, J.H Yoo, M.Y |
description | Conditions for the production of intracellular fructosyl transferase from A. pullulans were investigated. Sucrose was an excellent carbon source, and there was a tendency for the enzyme production to be increased as sucrose concentration was increased. Both 0.5% phosphate and 2% sodium nitrate had positive effects on enzyme production. It was possible to increase the intracellular enzyme production up to 140% by increasing the concentration of magnesium sulfate from 0.05% to 0.2%. |
doi_str_mv | 10.1007/BF01024601 |
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Sucrose was an excellent carbon source, and there was a tendency for the enzyme production to be increased as sucrose concentration was increased. Both 0.5% phosphate and 2% sodium nitrate had positive effects on enzyme production. It was possible to increase the intracellular enzyme production up to 140% by increasing the concentration of magnesium sulfate from 0.05% to 0.2%.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/BF01024601</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Aureobasidium pullulans ; Biological and medical sciences ; Biotechnology ; carbon sources ; enzyme activity ; Enzyme engineering ; Fermentation ; fructose ; fructosyl transferase ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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Sucrose was an excellent carbon source, and there was a tendency for the enzyme production to be increased as sucrose concentration was increased. Both 0.5% phosphate and 2% sodium nitrate had positive effects on enzyme production. It was possible to increase the intracellular enzyme production up to 140% by increasing the concentration of magnesium sulfate from 0.05% to 0.2%.</description><subject>Aureobasidium pullulans</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>carbon sources</subject><subject>enzyme activity</subject><subject>Enzyme engineering</subject><subject>Fermentation</subject><subject>fructose</subject><subject>fructosyl transferase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>nutrient requirements</subject><subject>phosphate</subject><subject>production</subject><subject>Production of selected enzymes</subject><subject>sodium nitrate</subject><subject>sucrose</subject><subject>transferases</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNotz01LAzEQBuAgCtbqxT_gHsTb6uRzdm_WYlUoKGjPS7KbyMpuU5PNof--gfY0w8vD8A4htxQeKQA-vayAAhMK6BmZUYm8VIjqnMyAClpKUbNLchXjHwDUCDgjz1_Bd6mder8tvCtcyLuP-6GYgt5GZ4OONqd-LBYpWG907Ls-jcUuDUMaMrkmF04P0d6c5pxsVq8_y_dy_fn2sVysS8dATqU2VWulMFJxXjmKhiFl1iHvdIfaOMkVOsU62zLmdC2qCo1xlXUcjeW05XPycLy7C_4_2Tg1Yx9bO-QO1qfYUIFScFpneH-COrZ6cPmPto_NLvSjDvsGhWKIVWZ3R-a0b_RvyGTzzYByYKBkzWt-AOpDYzU</recordid><startdate>19870101</startdate><enddate>19870101</enddate><creator>Jung, K.H</creator><creator>Lim, J.Y</creator><creator>Yoo, S.J</creator><creator>Lee, J.H</creator><creator>Yoo, M.Y</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19870101</creationdate><title>Production of fructosyl transferase from Aureobasidium pullulans</title><author>Jung, K.H ; Lim, J.Y ; Yoo, S.J ; Lee, J.H ; Yoo, M.Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f205t-ab8ce54b56338f17b2712ef73dad7abf5367f62dec22fa94887bbf8ef37be31c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Aureobasidium pullulans</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>carbon sources</topic><topic>enzyme activity</topic><topic>Enzyme engineering</topic><topic>Fermentation</topic><topic>fructose</topic><topic>fructosyl transferase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>nutrient requirements</topic><topic>phosphate</topic><topic>production</topic><topic>Production of selected enzymes</topic><topic>sodium nitrate</topic><topic>sucrose</topic><topic>transferases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jung, K.H</creatorcontrib><creatorcontrib>Lim, J.Y</creatorcontrib><creatorcontrib>Yoo, S.J</creatorcontrib><creatorcontrib>Lee, J.H</creatorcontrib><creatorcontrib>Yoo, M.Y</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jung, K.H</au><au>Lim, J.Y</au><au>Yoo, S.J</au><au>Lee, J.H</au><au>Yoo, M.Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of fructosyl transferase from Aureobasidium pullulans</atitle><jtitle>Biotechnology letters</jtitle><date>1987-01-01</date><risdate>1987</risdate><volume>9</volume><issue>10</issue><spage>703</spage><epage>708</epage><pages>703-708</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Conditions for the production of intracellular fructosyl transferase from A. pullulans were investigated. Sucrose was an excellent carbon source, and there was a tendency for the enzyme production to be increased as sucrose concentration was increased. Both 0.5% phosphate and 2% sodium nitrate had positive effects on enzyme production. It was possible to increase the intracellular enzyme production up to 140% by increasing the concentration of magnesium sulfate from 0.05% to 0.2%.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/BF01024601</doi><tpages>6</tpages></addata></record> |
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language | eng |
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source | Springer Nature - Complete Springer Journals |
subjects | Aureobasidium pullulans Biological and medical sciences Biotechnology carbon sources enzyme activity Enzyme engineering Fermentation fructose fructosyl transferase Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies nutrient requirements phosphate production Production of selected enzymes sodium nitrate sucrose transferases |
title | Production of fructosyl transferase from Aureobasidium pullulans |
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