Effects of paralytic shellfish poison on acetylcholine release from the myenteric plexus of guinea pig
We examined the mechanism of inhibition of acetylcholine (ACh) release by gonyautoxins (GTXs) in the longitudinal muscle strip preparation of guinea pig ileum. The preparation was placed in an organ-bath and immersed in Tyrode solution at a temperature of 37.0°C. ACh release from the myenteric plexu...
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| Veröffentlicht in: | Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 1986/12/05, Vol.27(6), pp.624-629_1 |
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| container_title | Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) |
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| creator | Chimi, K. (Japan Inst. of Oils and Fats, Other Foods Inspection, Tokyo) Taba, N Tachibana, M Katsuda, S Niiya, I |
| description | We examined the mechanism of inhibition of acetylcholine (ACh) release by gonyautoxins (GTXs) in the longitudinal muscle strip preparation of guinea pig ileum. The preparation was placed in an organ-bath and immersed in Tyrode solution at a temperature of 37.0°C. ACh release from the myenteric plexus of the longitudinal muscle was determined by using Palton's method. When the control Tyrode solution was replaced by the test solution containing GTXs (0.5MU/ml), spontaneous release of ACh was suppressed about 60% of the control value. GTXs completely inhibited ACh release induced by the application of nicotine (2.0μg/ml) or electrical stimuli (1 or 10Hz), whereas they did not affect ACh release evoked by the application of potassium-rich Tyrode solution (50mM). These results suggest that GTXs might suppress the impulse conduction of the post-synaptic neuron in the myenteric plexus. |
| doi_str_mv | 10.3358/shokueishi.27.624 |
| format | Article |
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(Japan Inst. of Oils and Fats, Other Foods Inspection, Tokyo) ; Taba, N ; Tachibana, M ; Katsuda, S ; Niiya, I</creator><creatorcontrib>Chimi, K. (Japan Inst. of Oils and Fats, Other Foods Inspection, Tokyo) ; Taba, N ; Tachibana, M ; Katsuda, S ; Niiya, I</creatorcontrib><description>We examined the mechanism of inhibition of acetylcholine (ACh) release by gonyautoxins (GTXs) in the longitudinal muscle strip preparation of guinea pig ileum. The preparation was placed in an organ-bath and immersed in Tyrode solution at a temperature of 37.0°C. ACh release from the myenteric plexus of the longitudinal muscle was determined by using Palton's method. When the control Tyrode solution was replaced by the test solution containing GTXs (0.5MU/ml), spontaneous release of ACh was suppressed about 60% of the control value. GTXs completely inhibited ACh release induced by the application of nicotine (2.0μg/ml) or electrical stimuli (1 or 10Hz), whereas they did not affect ACh release evoked by the application of potassium-rich Tyrode solution (50mM). These results suggest that GTXs might suppress the impulse conduction of the post-synaptic neuron in the myenteric plexus.</description><identifier>ISSN: 0015-6426</identifier><identifier>EISSN: 1882-1006</identifier><identifier>DOI: 10.3358/shokueishi.27.624</identifier><language>eng ; jpn</language><publisher>Japanese Society for Food Hygiene and Safety</publisher><subject>ACETILCOLINA ; ACETYLCHOLINE ; acetylcholine release ; COBAYE ; COBAYO ; COQUILLAGES ET CRUSTACES ; electrical stimuli ; Gonyaulax ; gonyautoxins ; guinea pig ; GUINEA PIGS ; longitudinal muscle strip preparation ; Marine ; MARISCOS ; nicotine ; PARALISIS ; PARALYSIE ; PARALYSIS ; PLEXO ; PLEXUS ; PLEXUS NERVEUX ; pottasium-rich Tyrode solution ; SHELLFISH ; TOXINAS ; TOXINE ; TOXINS</subject><ispartof>Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 1986/12/05, Vol.27(6), pp.624-629_1</ispartof><rights>The Food Hygienic Society of Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Chimi, K. 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When the control Tyrode solution was replaced by the test solution containing GTXs (0.5MU/ml), spontaneous release of ACh was suppressed about 60% of the control value. GTXs completely inhibited ACh release induced by the application of nicotine (2.0μg/ml) or electrical stimuli (1 or 10Hz), whereas they did not affect ACh release evoked by the application of potassium-rich Tyrode solution (50mM). These results suggest that GTXs might suppress the impulse conduction of the post-synaptic neuron in the myenteric plexus.</description><subject>ACETILCOLINA</subject><subject>ACETYLCHOLINE</subject><subject>acetylcholine release</subject><subject>COBAYE</subject><subject>COBAYO</subject><subject>COQUILLAGES ET CRUSTACES</subject><subject>electrical stimuli</subject><subject>Gonyaulax</subject><subject>gonyautoxins</subject><subject>guinea pig</subject><subject>GUINEA PIGS</subject><subject>longitudinal muscle strip preparation</subject><subject>Marine</subject><subject>MARISCOS</subject><subject>nicotine</subject><subject>PARALISIS</subject><subject>PARALYSIE</subject><subject>PARALYSIS</subject><subject>PLEXO</subject><subject>PLEXUS</subject><subject>PLEXUS NERVEUX</subject><subject>pottasium-rich Tyrode solution</subject><subject>SHELLFISH</subject><subject>TOXINAS</subject><subject>TOXINE</subject><subject>TOXINS</subject><issn>0015-6426</issn><issn>1882-1006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNpNkU1v1DAQhi1UJFalPwBOPvWWxV9xkmNVlZZqJTjA2XKcmY3BGwc7kbr_HtOgUskaX97nkeYdQj5wtpeybj_lMf5awefR70Wz10K9ITvetqLijOkLsmOM15VWQr8jVzn7njHWNUIKsSN4hwhuyTQinW2y4bx4R_MIIWAR0jn6HCdannWwnIMbY_AT0AQBbAaKKZ7oMgI9nWFaIBV4DvC0PguPa4laOvvje_IWbchw9e-_JD8-332_fagOX--_3N4cKqe4WipseomdFk5h1ze6G1o26JpJHATTAw664dYKrZTuJCDUUjnO-541KBFa1clLcr155xR_r5AXc_LZlWXsBHHNhqumlkVQgnwLuhRzToBmTv5k09lwZv6Wav6XakRjSqmFedyYn3mxR3ghbCqdBXhF8E6zZ2obBX4JudEmA1ORfdxkaKOxx-SzefzWMSbLbeQfEK2SeQ</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Chimi, K. 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(Japan Inst. of Oils and Fats, Other Foods Inspection, Tokyo)</creatorcontrib><creatorcontrib>Taba, N</creatorcontrib><creatorcontrib>Tachibana, M</creatorcontrib><creatorcontrib>Katsuda, S</creatorcontrib><creatorcontrib>Niiya, I</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chimi, K. 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ACh release from the myenteric plexus of the longitudinal muscle was determined by using Palton's method. When the control Tyrode solution was replaced by the test solution containing GTXs (0.5MU/ml), spontaneous release of ACh was suppressed about 60% of the control value. GTXs completely inhibited ACh release induced by the application of nicotine (2.0μg/ml) or electrical stimuli (1 or 10Hz), whereas they did not affect ACh release evoked by the application of potassium-rich Tyrode solution (50mM). These results suggest that GTXs might suppress the impulse conduction of the post-synaptic neuron in the myenteric plexus.</abstract><pub>Japanese Society for Food Hygiene and Safety</pub><doi>10.3358/shokueishi.27.624</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0015-6426 |
| ispartof | Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi), 1986/12/05, Vol.27(6), pp.624-629_1 |
| issn | 0015-6426 1882-1006 |
| language | eng ; jpn |
| recordid | cdi_proquest_miscellaneous_14753264 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | ACETILCOLINA ACETYLCHOLINE acetylcholine release COBAYE COBAYO COQUILLAGES ET CRUSTACES electrical stimuli Gonyaulax gonyautoxins guinea pig GUINEA PIGS longitudinal muscle strip preparation Marine MARISCOS nicotine PARALISIS PARALYSIE PARALYSIS PLEXO PLEXUS PLEXUS NERVEUX pottasium-rich Tyrode solution SHELLFISH TOXINAS TOXINE TOXINS |
| title | Effects of paralytic shellfish poison on acetylcholine release from the myenteric plexus of guinea pig |
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