Characterization of DNA polymerase activity in Trichoplusia ni cells infected with Autographa californica nuclear polyhedrosis virus
Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York 14853, U.S.A. Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral...
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| Veröffentlicht in: | Journal of general virology 1987-07, Vol.68 (7), p.2025-2031 |
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| container_issue | 7 |
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| container_title | Journal of general virology |
| container_volume | 68 |
| creator | Flore, P.H Burand, J.P Gettig, R.R Wood, H.A |
| description | Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York 14853, U.S.A.
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA:DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N -ethylmaleimide. The data indicate that the AcMNPV-specific DNA polymerase is a -like DNA polymerase which was first detected at 5 h post-inoculation.
Keywords: AcMNPV, DNA polymerase, hybridization, DNA:DNA
Received 16 December 1986;
accepted 30 March 1987. |
| doi_str_mv | 10.1099/0022-1317-68-7-2025 |
| format | Article |
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Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA:DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N -ethylmaleimide. The data indicate that the AcMNPV-specific DNA polymerase is a -like DNA polymerase which was first detected at 5 h post-inoculation.
Keywords: AcMNPV, DNA polymerase, hybridization, DNA:DNA
Received 16 December 1986;
accepted 30 March 1987.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-68-7-2025</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Autographa californica ; Biological and medical sciences ; biological control ; characterization ; DNA-directed DNA polymerase ; Fundamental and applied biological sciences. Psychology ; Microbiology ; Noctuidae ; nuclear polyhedrosis virus ; polyhedrosis viruses ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Trichoplusia ni ; Virology</subject><ispartof>Journal of general virology, 1987-07, Vol.68 (7), p.2025-2031</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-df4b61105d456bd2a1e2cb5852b27fef03eb62872a02652d2c059b7177e0476e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8356888$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Flore, P.H</creatorcontrib><creatorcontrib>Burand, J.P</creatorcontrib><creatorcontrib>Gettig, R.R</creatorcontrib><creatorcontrib>Wood, H.A</creatorcontrib><title>Characterization of DNA polymerase activity in Trichoplusia ni cells infected with Autographa californica nuclear polyhedrosis virus</title><title>Journal of general virology</title><description>Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York 14853, U.S.A.
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA:DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N -ethylmaleimide. The data indicate that the AcMNPV-specific DNA polymerase is a -like DNA polymerase which was first detected at 5 h post-inoculation.
Keywords: AcMNPV, DNA polymerase, hybridization, DNA:DNA
Received 16 December 1986;
accepted 30 March 1987.</description><subject>Autographa californica</subject><subject>Biological and medical sciences</subject><subject>biological control</subject><subject>characterization</subject><subject>DNA-directed DNA polymerase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Microbiology</subject><subject>Noctuidae</subject><subject>nuclear polyhedrosis virus</subject><subject>polyhedrosis viruses</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Trichoplusia ni</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpNkU1v1DAQhiNEJZaWX8ABHxDiErAdfyTH1QIFqSoH2rPlOOPNoGwc7KTV9swPr8NWFSdbmmcez7wuireMfmK0aT5TynnJKqZLVZe65JTLF8WGCSXzvWleFptn4lXxOqXflDIhpN4Uf3e9jdbNEPHBzhhGEjz5cr0lUxiOB4g2AcllvMP5SHAkNxFdH6ZhSWjJiMTBMKRc8JAdHbnHuSfbZQ77aKfeEmcH9CGO6DK9uAFs_GfuoYshYSJ3GJd0UZx5OyR483SeF7ffvt7svpdXPy9_7LZXpRO0mcvOi1YxRmUnpGo7bhlw18pa8pZrD55W0Cpea24pV5J33FHZtJppDVRoBdV58eHknWL4s0CazQHTuoEdISzJMKGFlLzOYHUCXZ4yRfBminiw8WgYNWviZs3TrHkaVRtt1sRz1_snvU15cR_t6DA9t9aVVHW9yj-esB73_T1GMHsYD5ifajGYHMj_xncn1Ntg7D5m2-0vTlmVv48rqnj1CDromoA</recordid><startdate>19870701</startdate><enddate>19870701</enddate><creator>Flore, P.H</creator><creator>Burand, J.P</creator><creator>Gettig, R.R</creator><creator>Wood, H.A</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>19870701</creationdate><title>Characterization of DNA polymerase activity in Trichoplusia ni cells infected with Autographa californica nuclear polyhedrosis virus</title><author>Flore, P.H ; Burand, J.P ; Gettig, R.R ; Wood, H.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-df4b61105d456bd2a1e2cb5852b27fef03eb62872a02652d2c059b7177e0476e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Autographa californica</topic><topic>Biological and medical sciences</topic><topic>biological control</topic><topic>characterization</topic><topic>DNA-directed DNA polymerase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Microbiology</topic><topic>Noctuidae</topic><topic>nuclear polyhedrosis virus</topic><topic>polyhedrosis viruses</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Trichoplusia ni</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flore, P.H</creatorcontrib><creatorcontrib>Burand, J.P</creatorcontrib><creatorcontrib>Gettig, R.R</creatorcontrib><creatorcontrib>Wood, H.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flore, P.H</au><au>Burand, J.P</au><au>Gettig, R.R</au><au>Wood, H.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of DNA polymerase activity in Trichoplusia ni cells infected with Autographa californica nuclear polyhedrosis virus</atitle><jtitle>Journal of general virology</jtitle><date>1987-07-01</date><risdate>1987</risdate><volume>68</volume><issue>7</issue><spage>2025</spage><epage>2031</epage><pages>2025-2031</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York 14853, U.S.A.
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA:DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N -ethylmaleimide. The data indicate that the AcMNPV-specific DNA polymerase is a -like DNA polymerase which was first detected at 5 h post-inoculation.
Keywords: AcMNPV, DNA polymerase, hybridization, DNA:DNA
Received 16 December 1986;
accepted 30 March 1987.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><doi>10.1099/0022-1317-68-7-2025</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of general virology, 1987-07, Vol.68 (7), p.2025-2031 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14745528 |
| source | Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Autographa californica Biological and medical sciences biological control characterization DNA-directed DNA polymerase Fundamental and applied biological sciences. Psychology Microbiology Noctuidae nuclear polyhedrosis virus polyhedrosis viruses Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Trichoplusia ni Virology |
| title | Characterization of DNA polymerase activity in Trichoplusia ni cells infected with Autographa californica nuclear polyhedrosis virus |
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