Respiratory electron transport in plasma and thylakoid membrane preparations from the cyanobacterium Anacystis nidulans
Plasma and thylakoid membranes were separated and purified from cell-free extracts of Anacystis nidulans by discontinuous sucrose density gradient centrifugation. Plasma membranes contained less than 0.05% (w/w) chlorophyll per protein. Both plasma and thylakoid membranes oxidized horse heart ferroc...
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| Veröffentlicht in: | FEBS letters 1986-01, Vol.195 (1), p.145-150 |
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| creator | Molitor, Veronique Peschek, Günter A. |
| description | Plasma and thylakoid membranes were separated and purified from cell-free extracts of
Anacystis nidulans by discontinuous sucrose density gradient centrifugation. Plasma membranes contained less than 0.05% (w/w) chlorophyll per protein. Both plasma and thylakoid membranes oxidized horse heart ferrocytochrome
c (10 ± 4 and 3 ± 1
nmol
mm
per mg protein, respectively), with strong inhibition by low concentrations of cyanide, sulfide, azide, carbon monoxide, and salicyl aldoxime. The activity was stimulated 125% by 3.5% Tween 80 and totally suppressed by 100 mM NaCl. Oxidized cytochrome
c was reduced by both types of membranes in the presence of NAD(P)H. The results indicate the occurrence of a respiratory chain including cytochrome-
c oxidase in both plasma and thylakoid membranes of
A. nidulans. |
| doi_str_mv | 10.1016/0014-5793(86)80149-1 |
| format | Article |
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Anacystis nidulans by discontinuous sucrose density gradient centrifugation. Plasma membranes contained less than 0.05% (w/w) chlorophyll per protein. Both plasma and thylakoid membranes oxidized horse heart ferrocytochrome
c (10 ± 4 and 3 ± 1
nmol
mm
per mg protein, respectively), with strong inhibition by low concentrations of cyanide, sulfide, azide, carbon monoxide, and salicyl aldoxime. The activity was stimulated 125% by 3.5% Tween 80 and totally suppressed by 100 mM NaCl. Oxidized cytochrome
c was reduced by both types of membranes in the presence of NAD(P)H. The results indicate the occurrence of a respiratory chain including cytochrome-
c oxidase in both plasma and thylakoid membranes of
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Anacystis nidulans by discontinuous sucrose density gradient centrifugation. Plasma membranes contained less than 0.05% (w/w) chlorophyll per protein. Both plasma and thylakoid membranes oxidized horse heart ferrocytochrome
c (10 ± 4 and 3 ± 1
nmol
mm
per mg protein, respectively), with strong inhibition by low concentrations of cyanide, sulfide, azide, carbon monoxide, and salicyl aldoxime. The activity was stimulated 125% by 3.5% Tween 80 and totally suppressed by 100 mM NaCl. Oxidized cytochrome
c was reduced by both types of membranes in the presence of NAD(P)H. The results indicate the occurrence of a respiratory chain including cytochrome-
c oxidase in both plasma and thylakoid membranes of
A. nidulans.</description><subject>Anacystis nidulans</subject><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Chl, chlorophyll</subject><subject>CM, plasma membrane</subject><subject>cyt, cytochrome</subject><subject>electron transport</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ICM, thylakoid membrane</subject><subject>Mitochondria and cell respiration</subject><subject>Molecular and cellular biology</subject><subject>plasma membranes</subject><subject>PMSF, phenylmethylsulfonyl fluoride</subject><subject>Respiratory chain Cytochrome-c oxidase Cytochrome c reductase Plasma membrane Thylakoid membrane (Anacystis nidulans) Cyanobacteria</subject><subject>thylakoid membranes</subject><subject>TLCK, N-α-p-tosyl-L-lysine chloromethyl ketone</subject><subject>TPCK, N-tosyl-Lphenylalanine chloromethyl ketone</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqNkU1rFTEUhoNU8Lb6D1xkIaKL0WQ-MslGqKVXhYIg7TpkkhOaOpOMOXMt8-_NeEuXpatwTp7z5vCEkLecfeKMi8-M8bbqetV8kOKjLIWq-Auy47JvqqYV8oTsHpFX5BTxjpVacrUj978A55DNkvJKYQS75BTpkk3EOeWFhkjn0eBkqImOLrfraH6n4OgE01AgoHOG2ZT5kCJSn9NUIKB2NTENxi6Qw2Gi59HYFZeANAZ3GEv4a_LSmxHhzcN5Rm72l9cX36urn99-XJxfVbZVDa86KQfR1WJwvTfKqZ51snGNV4pZ6ZxVXImuq2vXMSa8a2s1gFdWtp0tWO2bM_L-mDvn9OcAuOgpoIWx7ADpgJq3faOY4gVsj6DNCTGD13MOk8mr5kxvlvWmUG8KtRT6v2W9jb17yDdozeiLExvwcbaXQvaCFWx_xO7DCOuzovX-8mu9XWz9ErN1t_e-HIOgSPsbIGu0AaIFF3L5PO1SeHrhf3c3pqQ</recordid><startdate>19860120</startdate><enddate>19860120</enddate><creator>Molitor, Veronique</creator><creator>Peschek, Günter A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19860120</creationdate><title>Respiratory electron transport in plasma and thylakoid membrane preparations from the cyanobacterium Anacystis nidulans</title><author>Molitor, Veronique ; Peschek, Günter A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4931-588b6526bd7fa9d970583d3f990c8ddc91965522d5006fd429bef9c845c3d32f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Anacystis nidulans</topic><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>Chl, chlorophyll</topic><topic>CM, plasma membrane</topic><topic>cyt, cytochrome</topic><topic>electron transport</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ICM, thylakoid membrane</topic><topic>Mitochondria and cell respiration</topic><topic>Molecular and cellular biology</topic><topic>plasma membranes</topic><topic>PMSF, phenylmethylsulfonyl fluoride</topic><topic>Respiratory chain Cytochrome-c oxidase Cytochrome c reductase Plasma membrane Thylakoid membrane (Anacystis nidulans) Cyanobacteria</topic><topic>thylakoid membranes</topic><topic>TLCK, N-α-p-tosyl-L-lysine chloromethyl ketone</topic><topic>TPCK, N-tosyl-Lphenylalanine chloromethyl ketone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Molitor, Veronique</creatorcontrib><creatorcontrib>Peschek, Günter A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Molitor, Veronique</au><au>Peschek, Günter A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Respiratory electron transport in plasma and thylakoid membrane preparations from the cyanobacterium Anacystis nidulans</atitle><jtitle>FEBS letters</jtitle><date>1986-01-20</date><risdate>1986</risdate><volume>195</volume><issue>1</issue><spage>145</spage><epage>150</epage><pages>145-150</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Plasma and thylakoid membranes were separated and purified from cell-free extracts of
Anacystis nidulans by discontinuous sucrose density gradient centrifugation. Plasma membranes contained less than 0.05% (w/w) chlorophyll per protein. Both plasma and thylakoid membranes oxidized horse heart ferrocytochrome
c (10 ± 4 and 3 ± 1
nmol
mm
per mg protein, respectively), with strong inhibition by low concentrations of cyanide, sulfide, azide, carbon monoxide, and salicyl aldoxime. The activity was stimulated 125% by 3.5% Tween 80 and totally suppressed by 100 mM NaCl. Oxidized cytochrome
c was reduced by both types of membranes in the presence of NAD(P)H. The results indicate the occurrence of a respiratory chain including cytochrome-
c oxidase in both plasma and thylakoid membranes of
A. nidulans.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/0014-5793(86)80149-1</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1986-01, Vol.195 (1), p.145-150 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Anacystis nidulans Biological and medical sciences Cell structures and functions Chl, chlorophyll CM, plasma membrane cyt, cytochrome electron transport Fundamental and applied biological sciences. Psychology ICM, thylakoid membrane Mitochondria and cell respiration Molecular and cellular biology plasma membranes PMSF, phenylmethylsulfonyl fluoride Respiratory chain Cytochrome-c oxidase Cytochrome c reductase Plasma membrane Thylakoid membrane (Anacystis nidulans) Cyanobacteria thylakoid membranes TLCK, N-α-p-tosyl-L-lysine chloromethyl ketone TPCK, N-tosyl-Lphenylalanine chloromethyl ketone |
| title | Respiratory electron transport in plasma and thylakoid membrane preparations from the cyanobacterium Anacystis nidulans |
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