Omega Conus geographus toxin: A peptide that blocks calcium channels
We previously reported that omega Conus geographus toxin (ωCgTX), blocks evoked-release of transmitter at synapses in frog and attenuates the Ca 2+ component of the action potential of chick dorsal root ganglion neurons. We report here voltage-clamp experiments on cultured chick dorsal root ganglion...
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| Veröffentlicht in: | FEBS letters 1987-04, Vol.214 (2), p.295-300 |
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| creator | Feldman, Daniel H. Olivera, Baldomero M. Yoshikami, Doju |
| description | We previously reported that omega
Conus geographus toxin (ωCgTX), blocks evoked-release of transmitter at synapses in frog and attenuates the Ca
2+ component of the action potential of chick dorsal root ganglion neurons. We report here voltage-clamp experiments on cultured chick dorsal root ganglion neurons which demonstrate that ωCgTX produces a persistent block of voltage-gated Ca
2+ currents. Thus, we conclude that ωCgTX inhibits synaptic transmission by blocking Ca
2+ channels in the presynaptic nerve terminal. The toxin had no effect on K
+ currents; however, in some but not all neurons, ωCgTX reduced Na
+ currents by 10–25%. These findings suggest that ωCgTX should be useful as a probe to examine synaptic Ca
2+ channels. |
| doi_str_mv | 10.1016/0014-5793(87)80073-X |
| format | Article |
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Conus geographus toxin (ωCgTX), blocks evoked-release of transmitter at synapses in frog and attenuates the Ca
2+ component of the action potential of chick dorsal root ganglion neurons. We report here voltage-clamp experiments on cultured chick dorsal root ganglion neurons which demonstrate that ωCgTX produces a persistent block of voltage-gated Ca
2+ currents. Thus, we conclude that ωCgTX inhibits synaptic transmission by blocking Ca
2+ channels in the presynaptic nerve terminal. The toxin had no effect on K
+ currents; however, in some but not all neurons, ωCgTX reduced Na
+ currents by 10–25%. These findings suggest that ωCgTX should be useful as a probe to examine synaptic Ca
2+ channels.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(87)80073-X</identifier><identifier>PMID: 2436945</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; biological poisons ; Ca 2+ channel ; Ca2+ channel ; calcium ; Calcium - metabolism ; Cells, Cultured ; chickens ; Conus geographus ; dorsal root ganglia ; DRG, dorsal root ganglion ; Fundamental and applied biological sciences. Psychology ; Ganglia, Spinal - drug effects ; Ganglia, Spinal - metabolism ; Ion Channels - drug effects ; Ion Channels - metabolism ; Isolated neuron and nerve. Neuroglia ; Marine ; Mollusk Venoms - pharmacology ; Neurons - drug effects ; Neurons - metabolism ; Neurotoxin ; neurotoxins ; omega-Conotoxin GVIA ; Peptides - pharmacology ; Potassium - metabolism ; Sodium - metabolism ; Synapses - drug effects ; Synapses - metabolism ; TEA, tetraethylammonium ; toxins ; TTX, tetrodotoxin ; Vertebrates: nervous system and sense organs ; Voltage-clamp ; ωCgTX, omega Conus geographus toxin</subject><ispartof>FEBS letters, 1987-04, Vol.214 (2), p.295-300</ispartof><rights>1987</rights><rights>FEBS Letters 214 (1987) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526X-553ed460f99acb9db40f9e4b07d048699702b54858ce6e5a6b77828e50f50b783</citedby><cites>FETCH-LOGICAL-c526X-553ed460f99acb9db40f9e4b07d048699702b54858ce6e5a6b77828e50f50b783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(87)80073-X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27904,27905,45975</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7582689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2436945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feldman, Daniel H.</creatorcontrib><creatorcontrib>Olivera, Baldomero M.</creatorcontrib><creatorcontrib>Yoshikami, Doju</creatorcontrib><title>Omega Conus geographus toxin: A peptide that blocks calcium channels</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>We previously reported that omega
Conus geographus toxin (ωCgTX), blocks evoked-release of transmitter at synapses in frog and attenuates the Ca
2+ component of the action potential of chick dorsal root ganglion neurons. We report here voltage-clamp experiments on cultured chick dorsal root ganglion neurons which demonstrate that ωCgTX produces a persistent block of voltage-gated Ca
2+ currents. Thus, we conclude that ωCgTX inhibits synaptic transmission by blocking Ca
2+ channels in the presynaptic nerve terminal. The toxin had no effect on K
+ currents; however, in some but not all neurons, ωCgTX reduced Na
+ currents by 10–25%. These findings suggest that ωCgTX should be useful as a probe to examine synaptic Ca
2+ channels.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>biological poisons</subject><subject>Ca 2+ channel</subject><subject>Ca2+ channel</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>chickens</subject><subject>Conus geographus</subject><subject>dorsal root ganglia</subject><subject>DRG, dorsal root ganglion</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganglia, Spinal - drug effects</subject><subject>Ganglia, Spinal - metabolism</subject><subject>Ion Channels - drug effects</subject><subject>Ion Channels - metabolism</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Marine</subject><subject>Mollusk Venoms - pharmacology</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Neurotoxin</subject><subject>neurotoxins</subject><subject>omega-Conotoxin GVIA</subject><subject>Peptides - pharmacology</subject><subject>Potassium - metabolism</subject><subject>Sodium - metabolism</subject><subject>Synapses - drug effects</subject><subject>Synapses - metabolism</subject><subject>TEA, tetraethylammonium</subject><subject>toxins</subject><subject>TTX, tetrodotoxin</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Voltage-clamp</subject><subject>ωCgTX, omega Conus geographus toxin</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtP3DAUha0KBFPgHxQpC1S1i1A78bOLSsOUgUpIbECaneU4NzNu88JOSvn3dZjRLBErX99z7rn2h9Angi8JJvwbxoSmTKj8ixRfJcYiT1cf0IzIWOSUywM021uO0ccQfuN4l0QdoaOM5lxRNkM_7xtYm2TRtWNI1tCtvek3sRy6f679nsyTHvrBlZAMGzMkRd3ZPyGxprZubBK7MW0LdThFh5WpA5ztzhP0uLx-WNymd_c3vxbzu9SyjK9SxnIoKceVUsYWqixoLIEWWJSYSq6UwFnBqGTSAgdmeCGEzCQwXDFcCJmfoM_b3N53TyOEQTcuWKhr00I3Bk2oyDHJWDTSrdH6LgQPle69a4x_0QTrCZ6eyOiJjJZCv8LTqzh2vssfiwbK_dCOVtQvdroJkUHlTWtd2NsEkxmXKtqWW9uzq-HlXav18voqm4SpL8Vrd3rPj21QZAx_HXgdrIPWQuk82EGXnXv7Q_8BNBydUA</recordid><startdate>19870420</startdate><enddate>19870420</enddate><creator>Feldman, Daniel H.</creator><creator>Olivera, Baldomero M.</creator><creator>Yoshikami, Doju</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19870420</creationdate><title>Omega Conus geographus toxin: A peptide that blocks calcium channels</title><author>Feldman, Daniel H. ; Olivera, Baldomero M. ; Yoshikami, Doju</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526X-553ed460f99acb9db40f9e4b07d048699702b54858ce6e5a6b77828e50f50b783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>biological poisons</topic><topic>Ca 2+ channel</topic><topic>Ca2+ channel</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>chickens</topic><topic>Conus geographus</topic><topic>dorsal root ganglia</topic><topic>DRG, dorsal root ganglion</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ganglia, Spinal - drug effects</topic><topic>Ganglia, Spinal - metabolism</topic><topic>Ion Channels - drug effects</topic><topic>Ion Channels - metabolism</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Marine</topic><topic>Mollusk Venoms - pharmacology</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Neurotoxin</topic><topic>neurotoxins</topic><topic>omega-Conotoxin GVIA</topic><topic>Peptides - pharmacology</topic><topic>Potassium - metabolism</topic><topic>Sodium - metabolism</topic><topic>Synapses - drug effects</topic><topic>Synapses - metabolism</topic><topic>TEA, tetraethylammonium</topic><topic>toxins</topic><topic>TTX, tetrodotoxin</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Voltage-clamp</topic><topic>ωCgTX, omega Conus geographus toxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feldman, Daniel H.</creatorcontrib><creatorcontrib>Olivera, Baldomero M.</creatorcontrib><creatorcontrib>Yoshikami, Doju</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feldman, Daniel H.</au><au>Olivera, Baldomero M.</au><au>Yoshikami, Doju</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Omega Conus geographus toxin: A peptide that blocks calcium channels</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1987-04-20</date><risdate>1987</risdate><volume>214</volume><issue>2</issue><spage>295</spage><epage>300</epage><pages>295-300</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>We previously reported that omega
Conus geographus toxin (ωCgTX), blocks evoked-release of transmitter at synapses in frog and attenuates the Ca
2+ component of the action potential of chick dorsal root ganglion neurons. We report here voltage-clamp experiments on cultured chick dorsal root ganglion neurons which demonstrate that ωCgTX produces a persistent block of voltage-gated Ca
2+ currents. Thus, we conclude that ωCgTX inhibits synaptic transmission by blocking Ca
2+ channels in the presynaptic nerve terminal. The toxin had no effect on K
+ currents; however, in some but not all neurons, ωCgTX reduced Na
+ currents by 10–25%. These findings suggest that ωCgTX should be useful as a probe to examine synaptic Ca
2+ channels.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2436945</pmid><doi>10.1016/0014-5793(87)80073-X</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1987-04, Vol.214 (2), p.295-300 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences biological poisons Ca 2+ channel Ca2+ channel calcium Calcium - metabolism Cells, Cultured chickens Conus geographus dorsal root ganglia DRG, dorsal root ganglion Fundamental and applied biological sciences. Psychology Ganglia, Spinal - drug effects Ganglia, Spinal - metabolism Ion Channels - drug effects Ion Channels - metabolism Isolated neuron and nerve. Neuroglia Marine Mollusk Venoms - pharmacology Neurons - drug effects Neurons - metabolism Neurotoxin neurotoxins omega-Conotoxin GVIA Peptides - pharmacology Potassium - metabolism Sodium - metabolism Synapses - drug effects Synapses - metabolism TEA, tetraethylammonium toxins TTX, tetrodotoxin Vertebrates: nervous system and sense organs Voltage-clamp ωCgTX, omega Conus geographus toxin |
| title | Omega Conus geographus toxin: A peptide that blocks calcium channels |
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