Effect of tobacco mosaic virus infection of levels of soluble superoxide dismutase (SOD) in nicotiana tabacum and nicotiana glutinosa leaves
The activity of superoxide dismutase (SOD: E.C. 1.15.1.1) was evaluated on Nicotiana tabacum and Nicotiana glutinosa leaf tissue after Tobacco Mosaic Virus (TMV) infection. Significant increase in extracted SOD appeared to be directly related to the appearance of necrotic and systemic symptoms in hy...
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| Veröffentlicht in: | Plant science (Limerick) 1986, Vol.47 (2), p.135-143 |
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| creator | Montalbini, Paolo Buonaurio, Roberto |
| description | The activity of superoxide dismutase (SOD: E.C. 1.15.1.1) was evaluated on
Nicotiana tabacum and
Nicotiana glutinosa leaf tissue after Tobacco Mosaic Virus (TMV) infection. Significant increase in extracted SOD appeared to be directly related to the appearance of necrotic and systemic symptoms in hypersensitive (
N. glutinosa and
N. tabacum cv. Havana 425) and susceptible (
N. tabacum cv. Bright BC 60) leaves, respectively. SOD activity did not change significantly during the replication of TMV in the inoculated susceptible leaves up to 4 days after inoculation. Both cyanide-insensitive (2 days after inoculation) and sensitive (3–4 days after inoculation) enzymes increased during the expression of the hypersensitivity. Only cyanide-sensitive enzyme increased in systematically infected leaves. SOD and peroxidase increased simultaneously and the enhancement of peroxidase was higher than that of SOD. The values of peroxidase greatly exceeded that of SOD only in the hypersensitive leaves during local lesion differentiation. In
N. tabacum leaves 4 clear SOD bands were separated by polyacrylamide gel electrophoresis: 3 cyanide-sensitive (Cu,Zn enzyme) and 1 cyanide-insensitive, while
N. glutinosa had 3 bands: 2 cyanide-sensitive and 1 cyanide-insensitive. The cyanide-insensitive band, both in
N. tabacum and
N. glutinosa, was sensitive to H
2O
2 and insensitive to chloroform-ethanol treatment and thus supposed to be Fe enzyme. The infection did not induce change in the electrophoretic pattern of SOD enzymes.
In summary, our results indicate that the pathogenic alteration caused by TMV infection both in the compatible and in the incompatible combinations are characterized by an induction of SOD activity, particularly cyanide-sensitive Cu,Zn-SOD. The connection between the induction of SOD and a possible activation of O
2
− production in the hypersensitive tissue following TMV infection is discussed. |
| doi_str_mv | 10.1016/0168-9452(86)90060-9 |
| format | Article |
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Nicotiana tabacum and
Nicotiana glutinosa leaf tissue after Tobacco Mosaic Virus (TMV) infection. Significant increase in extracted SOD appeared to be directly related to the appearance of necrotic and systemic symptoms in hypersensitive (
N. glutinosa and
N. tabacum cv. Havana 425) and susceptible (
N. tabacum cv. Bright BC 60) leaves, respectively. SOD activity did not change significantly during the replication of TMV in the inoculated susceptible leaves up to 4 days after inoculation. Both cyanide-insensitive (2 days after inoculation) and sensitive (3–4 days after inoculation) enzymes increased during the expression of the hypersensitivity. Only cyanide-sensitive enzyme increased in systematically infected leaves. SOD and peroxidase increased simultaneously and the enhancement of peroxidase was higher than that of SOD. The values of peroxidase greatly exceeded that of SOD only in the hypersensitive leaves during local lesion differentiation. In
N. tabacum leaves 4 clear SOD bands were separated by polyacrylamide gel electrophoresis: 3 cyanide-sensitive (Cu,Zn enzyme) and 1 cyanide-insensitive, while
N. glutinosa had 3 bands: 2 cyanide-sensitive and 1 cyanide-insensitive. The cyanide-insensitive band, both in
N. tabacum and
N. glutinosa, was sensitive to H
2O
2 and insensitive to chloroform-ethanol treatment and thus supposed to be Fe enzyme. The infection did not induce change in the electrophoretic pattern of SOD enzymes.
In summary, our results indicate that the pathogenic alteration caused by TMV infection both in the compatible and in the incompatible combinations are characterized by an induction of SOD activity, particularly cyanide-sensitive Cu,Zn-SOD. The connection between the induction of SOD and a possible activation of O
2
− production in the hypersensitive tissue following TMV infection is discussed.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/0168-9452(86)90060-9</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; hypersensitivity ; leaves ; Nicotiana glutinosa ; Nicotiana tabacum ; pathogenesis ; Pathology. Damages, economic importance ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; superoxide dismutase ; susceptibility ; Tobacco Mosaic Virus</subject><ispartof>Plant science (Limerick), 1986, Vol.47 (2), p.135-143</ispartof><rights>1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-4bcd8774c07d34f9433e745a9696e763201c6367ecb09352a944e0b6c764596b3</citedby><cites>FETCH-LOGICAL-c388t-4bcd8774c07d34f9433e745a9696e763201c6367ecb09352a944e0b6c764596b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-9452(86)90060-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8247950$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Montalbini, Paolo</creatorcontrib><creatorcontrib>Buonaurio, Roberto</creatorcontrib><title>Effect of tobacco mosaic virus infection of levels of soluble superoxide dismutase (SOD) in nicotiana tabacum and nicotiana glutinosa leaves</title><title>Plant science (Limerick)</title><description>The activity of superoxide dismutase (SOD: E.C. 1.15.1.1) was evaluated on
Nicotiana tabacum and
Nicotiana glutinosa leaf tissue after Tobacco Mosaic Virus (TMV) infection. Significant increase in extracted SOD appeared to be directly related to the appearance of necrotic and systemic symptoms in hypersensitive (
N. glutinosa and
N. tabacum cv. Havana 425) and susceptible (
N. tabacum cv. Bright BC 60) leaves, respectively. SOD activity did not change significantly during the replication of TMV in the inoculated susceptible leaves up to 4 days after inoculation. Both cyanide-insensitive (2 days after inoculation) and sensitive (3–4 days after inoculation) enzymes increased during the expression of the hypersensitivity. Only cyanide-sensitive enzyme increased in systematically infected leaves. SOD and peroxidase increased simultaneously and the enhancement of peroxidase was higher than that of SOD. The values of peroxidase greatly exceeded that of SOD only in the hypersensitive leaves during local lesion differentiation. In
N. tabacum leaves 4 clear SOD bands were separated by polyacrylamide gel electrophoresis: 3 cyanide-sensitive (Cu,Zn enzyme) and 1 cyanide-insensitive, while
N. glutinosa had 3 bands: 2 cyanide-sensitive and 1 cyanide-insensitive. The cyanide-insensitive band, both in
N. tabacum and
N. glutinosa, was sensitive to H
2O
2 and insensitive to chloroform-ethanol treatment and thus supposed to be Fe enzyme. The infection did not induce change in the electrophoretic pattern of SOD enzymes.
In summary, our results indicate that the pathogenic alteration caused by TMV infection both in the compatible and in the incompatible combinations are characterized by an induction of SOD activity, particularly cyanide-sensitive Cu,Zn-SOD. The connection between the induction of SOD and a possible activation of O
2
− production in the hypersensitive tissue following TMV infection is discussed.</description><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hypersensitivity</subject><subject>leaves</subject><subject>Nicotiana glutinosa</subject><subject>Nicotiana tabacum</subject><subject>pathogenesis</subject><subject>Pathology. Damages, economic importance</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>superoxide dismutase</subject><subject>susceptibility</subject><subject>Tobacco Mosaic Virus</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNp9kc9qFTEUxoMoeK2-gWAWIu1iNJn8m2wEqW0VCl3UrkMmc6ZEZpJrzsxF38GHNuMtxZWLwwk5v-_L4Qshrzl7zxnXH2p1jZWqPe30mWVMs8Y-ITveGdG0rbJPye4ReU5eIH5njLVKmR35fTGOEBaaR7rk3oeQ6ZzRx0APsaxIY9rGMaeNmOAAE24nzNPaT0Bx3UPJP-MAdIg4r4tHoKe3N5_PqpKmGPISffJ08dV7nalPwz-399O6xFSfq87-APiSPBv9hPDqoZ-Qu8uLb-dfmuubq6_nn66bILpuaWQfhs4YGZgZhBytFAKMVN5qq8Fo0TIetNAGQs-sUK23UgLrdTBaKqt7cULeHX33Jf9YARc3RwwwTT5BXtFxaRirVUF5BEPJiAVGty9x9uWX48xt0bstV7fl6jrt_kbvbJW9ffD3GPw0Fp9CxEdt10pj1eb-5oiNPjt_Xypyd1uXF4xLroQSlfh4JGrscIhQHIYIKcAQS_0WN-T4_03-APtcocs</recordid><startdate>1986</startdate><enddate>1986</enddate><creator>Montalbini, Paolo</creator><creator>Buonaurio, Roberto</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>1986</creationdate><title>Effect of tobacco mosaic virus infection of levels of soluble superoxide dismutase (SOD) in nicotiana tabacum and nicotiana glutinosa leaves</title><author>Montalbini, Paolo ; Buonaurio, Roberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-4bcd8774c07d34f9433e745a9696e763201c6367ecb09352a944e0b6c764596b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hypersensitivity</topic><topic>leaves</topic><topic>Nicotiana glutinosa</topic><topic>Nicotiana tabacum</topic><topic>pathogenesis</topic><topic>Pathology. Damages, economic importance</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant viruses and viroids</topic><topic>superoxide dismutase</topic><topic>susceptibility</topic><topic>Tobacco Mosaic Virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Montalbini, Paolo</creatorcontrib><creatorcontrib>Buonaurio, Roberto</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Montalbini, Paolo</au><au>Buonaurio, Roberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of tobacco mosaic virus infection of levels of soluble superoxide dismutase (SOD) in nicotiana tabacum and nicotiana glutinosa leaves</atitle><jtitle>Plant science (Limerick)</jtitle><date>1986</date><risdate>1986</risdate><volume>47</volume><issue>2</issue><spage>135</spage><epage>143</epage><pages>135-143</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>The activity of superoxide dismutase (SOD: E.C. 1.15.1.1) was evaluated on
Nicotiana tabacum and
Nicotiana glutinosa leaf tissue after Tobacco Mosaic Virus (TMV) infection. Significant increase in extracted SOD appeared to be directly related to the appearance of necrotic and systemic symptoms in hypersensitive (
N. glutinosa and
N. tabacum cv. Havana 425) and susceptible (
N. tabacum cv. Bright BC 60) leaves, respectively. SOD activity did not change significantly during the replication of TMV in the inoculated susceptible leaves up to 4 days after inoculation. Both cyanide-insensitive (2 days after inoculation) and sensitive (3–4 days after inoculation) enzymes increased during the expression of the hypersensitivity. Only cyanide-sensitive enzyme increased in systematically infected leaves. SOD and peroxidase increased simultaneously and the enhancement of peroxidase was higher than that of SOD. The values of peroxidase greatly exceeded that of SOD only in the hypersensitive leaves during local lesion differentiation. In
N. tabacum leaves 4 clear SOD bands were separated by polyacrylamide gel electrophoresis: 3 cyanide-sensitive (Cu,Zn enzyme) and 1 cyanide-insensitive, while
N. glutinosa had 3 bands: 2 cyanide-sensitive and 1 cyanide-insensitive. The cyanide-insensitive band, both in
N. tabacum and
N. glutinosa, was sensitive to H
2O
2 and insensitive to chloroform-ethanol treatment and thus supposed to be Fe enzyme. The infection did not induce change in the electrophoretic pattern of SOD enzymes.
In summary, our results indicate that the pathogenic alteration caused by TMV infection both in the compatible and in the incompatible combinations are characterized by an induction of SOD activity, particularly cyanide-sensitive Cu,Zn-SOD. The connection between the induction of SOD and a possible activation of O
2
− production in the hypersensitive tissue following TMV infection is discussed.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/0168-9452(86)90060-9</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0168-9452 |
| ispartof | Plant science (Limerick), 1986, Vol.47 (2), p.135-143 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Fundamental and applied biological sciences. Psychology hypersensitivity leaves Nicotiana glutinosa Nicotiana tabacum pathogenesis Pathology. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids superoxide dismutase susceptibility Tobacco Mosaic Virus |
| title | Effect of tobacco mosaic virus infection of levels of soluble superoxide dismutase (SOD) in nicotiana tabacum and nicotiana glutinosa leaves |
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