Location of a second partitioning region (ParL) on the F plasmid

Location of a second partitioning region (ParL) on the F plasmid

The leading region of the F plasmid has been found to extend the maintenance of the normally unstable plasmid vector pACYC184. This ability is due to effective partitioning of plasmid molecules at cell division. Cloning, deletion analysis and transposon mutagenesis have located the partitioning regi...

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Veröffentlicht in:FEMS microbiology letters 1986-11, Vol.37 (2), p.179-182
Hauptverfasser: Loh, S.M., Ray, A., Cram, D.S., O'Gorman, L.E., Skurray, R.A.
Format: Artikel
Sprache:eng
Schlagworte:
Bacteriology
Biological and medical sciences
Escherichia coli
F plasmid
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
partitioning region
plasmid stability
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Beschreibung
Zusammenfassung:The leading region of the F plasmid has been found to extend the maintenance of the normally unstable plasmid vector pACYC184. This ability is due to effective partitioning of plasmid molecules at cell division. Cloning, deletion analysis and transposon mutagenesis have located the partitioning region (ParL) to be encoded within 63.65–64.11F. Complementation studies indicated that parL is a cis‐acting locus.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1986.tb01789.x