CTR1 Silencing Inhibits Angiogenesis by Limiting Copper Entry into Endothelial Cells: e71982

CTR1 Silencing Inhibits Angiogenesis by Limiting Copper Entry into Endothelial Cells: e71982

Increased levels of intracellular copper stimulate angiogenesis in human umbilical vein endothelial cells (HUVECs). Copper transporter 1 (CTR1) is a copper importer present in the cell membrane and plays a major role in copper transport. In this study, three siRNAs targeting CTR1 mRNA were designed...

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Veröffentlicht in:PloS one 2013-09, Vol.8 (9)
Hauptverfasser: Narayanan, Gomathy, R, Bharathidevi S, Vuyyuru, Harish, Muthuvel, Bharathselvi, Natrajan, Sulochana Konerirajapuram
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R, Bharathidevi S
Vuyyuru, Harish
Muthuvel, Bharathselvi
Natrajan, Sulochana Konerirajapuram
description Increased levels of intracellular copper stimulate angiogenesis in human umbilical vein endothelial cells (HUVECs). Copper transporter 1 (CTR1) is a copper importer present in the cell membrane and plays a major role in copper transport. In this study, three siRNAs targeting CTR1 mRNA were designed and screened for gene silencing. HUVECs when exposed to 100 mu M copper showed 3 fold increased proliferation, migration by 1.8 - fold and tube formation by 1.8 - fold. One of the designed CTR1 siRNA (si 1) at 10 nM concentration decreased proliferation by 2.5 - fold, migration by 4 - fold and tube formation by 2.8 - fold. Rabbit corneal packet assay also showed considerable decrease in matrigel induced blood vessel formation by si 1 when compared to untreated control. The designed si 1 when topically applied inhibited angiogenesis. This can be further developed for therapeutic application.
doi_str_mv 10.1371/journal.pone.0071982
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title CTR1 Silencing Inhibits Angiogenesis by Limiting Copper Entry into Endothelial Cells: e71982
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